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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0662 [new locus tag: SACOL_RS03425 ]
  • pan locus tag?: SAUPAN002472000
  • symbol: SACOL0662
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0662 [new locus tag: SACOL_RS03425 ]
  • symbol: SACOL0662
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 690216..690644
  • length: 429
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    GTGAGTAGTAAATTGGATAAAAAAGTAAGTATTCAAACAAAGCAAGTGTTGAAACAGCAC
    AACGAAAAAGAAAAATTTGAATTTACTACTGAAGGAACTTGGCAACAAAGGCAATCTAAC
    TTTATTCGGTATGTAGAACAAATTGAGGATGCAACAGTTAATGTTACAATAAAAGTGGAT
    GATGATAGCGTTAAGTTGATTCGTAAAGGCGACATTAATATGAATTTGCATTTTGTTGAA
    GGACAAACGACAACAACTTTTTACGATATATCGGCTGGACGAATTCCACTAGAAGTTAAA
    ACATTACGCATTTTACATTTCGTAAGTGGAGACGGTGGCAAGCTAAAGATTCATTATGAA
    TTATATCAAGATAATGAAAAAATGGGTTCTTATCAATATGAAATTAACTATAAGGAGATA
    GGCGAATGA
    60
    120
    180
    240
    300
    360
    420
    429

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0662 [new locus tag: SACOL_RS03425 ]
  • symbol: SACOL0662
  • description: hypothetical protein
  • length: 142
  • theoretical pI: 6.52603
  • theoretical MW: 16598.6
  • GRAVY: -0.727465

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • hypothetical fig|282458.1.peg.583 homolog
  • PFAM:
    Calycin (CL0116) DUF1934; Domain of unknown function (DUF1934) (PF09148; HMM-score: 106.4)
    and 2 more
    DUF4488; Domain of unknown function (DUF4488) (PF14869; HMM-score: 15.7)
    BPD_transp_1 (CL0404) YjgP_YjgQ; Predicted permease YjgP/YjgQ family (PF03739; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00323
    • TAT(Tat/SPI): 0.000261
    • LIPO(Sec/SPII): 0.000479
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSKLDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQIEDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILHFVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SACOL0452(ahpC)alkyl hydroperoxide reductase subunit C  [3] (data from MRSA252)
    SACOL2074(ddl)D-alanyl-alanine synthetase A  [3] (data from MRSA252)
    SACOL2130(deoD)purine nucleoside phosphorylase  [3] (data from MRSA252)
    SACOL0937(dltC)D-alanine--poly(phosphoribitol) ligase subunit 2  [3] (data from MRSA252)
    SACOL1637(dnaK)molecular chaperone DnaK  [3] (data from MRSA252)
    SACOL0842(eno)phosphopyruvate hydratase  [3] (data from MRSA252)
    SACOL1245(fabG1)3-oxoacyl-ACP reductase  [3] (data from MRSA252)
    SACOL1016(fabI)enoyl-ACP reductase  [3] (data from MRSA252)
    SACOL2117(fbaA)fructose-bisphosphate aldolase  [3] (data from MRSA252)
    SACOL1329(femC)glutamine synthetase  [3] (data from MRSA252)
    SACOL1072(folD)bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase  [3] (data from MRSA252)
    SACOL1199(ftsZ)cell division protein FtsZ  [3] (data from MRSA252)
    SACOL0593(fusA)elongation factor G  [3] (data from MRSA252)
    SACOL1734(gapA2)glyceraldehyde 3-phosphate dehydrogenase 2  [3] (data from MRSA252)
    SACOL1961(gatA)aspartyl/glutamyl-tRNA amidotransferase subunit A  [3] (data from MRSA252)
    SACOL1595(gcvT)glycine cleavage system aminomethyltransferase T  [3] (data from MRSA252)
    SACOL1622(glyS)glycyl-tRNA synthetase  [3] (data from MRSA252)
    SACOL2016(groEL)chaperonin GroEL  [3] (data from MRSA252)
    SACOL2017(groES)co-chaperonin GroES  [3] (data from MRSA252)
    SACOL1513(hup)DNA-binding protein HU  [3] (data from MRSA252)
    SACOL1741(icd)isocitrate dehydrogenase  [3] (data from MRSA252)
    SACOL1206(ileS)isoleucyl-tRNA synthetase  [3] (data from MRSA252)
    SACOL1288(infB)translation initiation factor IF-2  [3] (data from MRSA252)
    SACOL1727(infC)translation initiation factor IF-3  [3] (data from MRSA252)
    SACOL2623(mqo2)malate:quinone oxidoreductase  [3] (data from MRSA252)
    SACOL2116(murAB)UDP-N-acetylglucosamine 1-carboxyvinyltransferase  [3] (data from MRSA252)
    SACOL0582(nusG)transcription antitermination protein  [3] (data from MRSA252)
    SACOL1102(pdhA)pyruvate dehydrogenase complex E1 component subunit alpha  [3] (data from MRSA252)
    SACOL1103(pdhB)pyruvate dehydrogenase complex E1 component subunit beta  [3] (data from MRSA252)
    SACOL1104(pdhC)branched-chain alpha-keto acid dehydrogenase E2  [3] (data from MRSA252)
    SACOL1105(pdhD)dihydrolipoamide dehydrogenase  [3] (data from MRSA252)
    SACOL2128(pdp)pyrimidine-nucleoside phosphorylase  [3] (data from MRSA252)
    SACOL1005(pepF)oligoendopeptidase F  [3] (data from MRSA252)
    SACOL0204(pflB)formate acetyltransferase  [3] (data from MRSA252)
    SACOL0966(pgi)glucose-6-phosphate isomerase  [3] (data from MRSA252)
    SACOL0839(pgk)phosphoglycerate kinase  [3] (data from MRSA252)
    SACOL0841(pgm)phosphoglyceromutase  [3] (data from MRSA252)
    SACOL1091(ptsH)phosphocarrier protein HPr  [3] (data from MRSA252)
    SACOL1092(ptsI)phosphoenolpyruvate-protein phosphotransferase  [3] (data from MRSA252)
    SACOL1816(putA)proline dehydrogenase  [3] (data from MRSA252)
    SACOL1745(pyk)pyruvate kinase  [3] (data from MRSA252)
    SACOL0584(rplA)50S ribosomal protein L1  [3] (data from MRSA252)
    SACOL2236(rplB)50S ribosomal protein L2  [3] (data from MRSA252)
    SACOL2239(rplC)50S ribosomal protein L3  [3] (data from MRSA252)
    SACOL2238(rplD)50S ribosomal protein L4  [3] (data from MRSA252)
    SACOL2227(rplE)50S ribosomal protein L5  [3] (data from MRSA252)
    SACOL2224(rplF)50S ribosomal protein L6  [3] (data from MRSA252)
    SACOL0585(rplJ)50S ribosomal protein L10  [3] (data from MRSA252)
    SACOL0586(rplL)50S ribosomal protein L7/L12  [3] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [3] (data from MRSA252)
    SACOL2232(rplP)50S ribosomal protein L16  [3] (data from MRSA252)
    SACOL1257(rplS)50S ribosomal protein L19  [3] (data from MRSA252)
    SACOL1702(rplU)50S ribosomal protein L21  [3] (data from MRSA252)
    SACOL2234(rplV)50S ribosomal protein L22  [3] (data from MRSA252)
    SACOL2237(rplW)50S ribosomal protein L23  [3] (data from MRSA252)
    SACOL2228(rplX)50S ribosomal protein L24  [3] (data from MRSA252)
    SACOL0545(rplY)50S ribosomal protein L25/general stress protein Ctc  [3] (data from MRSA252)
    SACOL2112(rpmE2)50S ribosomal protein L31  [3] (data from MRSA252)
    SACOL1618(rpoD)RNA polymerase sigma factor RpoD  [3] (data from MRSA252)
    SACOL1274(rpsB)30S ribosomal protein S2  [3] (data from MRSA252)
    SACOL2233(rpsC)30S ribosomal protein S3  [3] (data from MRSA252)
    SACOL1769(rpsD)30S ribosomal protein S4  [3] (data from MRSA252)
    SACOL2222(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)
    SACOL0592(rpsG)30S ribosomal protein S7  [3] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [3] (data from MRSA252)
    SACOL2214(rpsK)30S ribosomal protein S11  [3] (data from MRSA252)
    SACOL2235(rpsS)30S ribosomal protein S19  [3] (data from MRSA252)
    SACOL1642(rpsT)30S ribosomal protein S20  [3] (data from MRSA252)
    SACOL0816(secA)preprotein translocase subunit SecA  [3] (data from MRSA252)
    SACOL1610(sodA2)superoxide dismutase  [3] (data from MRSA252)
    SACOL0095(spa)immunoglobulin G binding protein A precursor  [3] (data from MRSA252)
    SACOL1449(sucA)2-oxoglutarate dehydrogenase E1 component  [3] (data from MRSA252)
    SACOL1448(sucB)dihydrolipoamide succinyltransferase  [3] (data from MRSA252)
    SACOL1262(sucC)succinyl-CoA synthetase subunit beta  [3] (data from MRSA252)
    SACOL1831(tal)translaldolase  [3] (data from MRSA252)
    SACOL1729(thrS)threonyl-tRNA synthetase  [3] (data from MRSA252)
    SACOL1722(tig)trigger factor  [3] (data from MRSA252)
    SACOL0840(tpiA)triosephosphate isomerase  [3] (data from MRSA252)
    SACOL1155(trxA)thioredoxin  [3] (data from MRSA252)
    SACOL0594(tuf)elongation factor Tu  [3] (data from MRSA252)
    SACOL2104(upp)uracil phosphoribosyltransferase  [3] (data from MRSA252)
    SACOL0444hypothetical protein  [3] (data from MRSA252)
    SACOL0617hexulose-6-phosphate synthase  [3] (data from MRSA252)
    SACOL0721hypothetical protein  [3] (data from MRSA252)
    SACOL0731LysR family transcriptional regulator  [3] (data from MRSA252)
    SACOL0742hypothetical protein  [3] (data from MRSA252)
    SACOL0815ribosomal subunit interface protein  [3] (data from MRSA252)
    SACOL0876hypothetical protein  [3] (data from MRSA252)
    SACOL0914FeS assembly ATPase SufC  [3] (data from MRSA252)
    SACOL0944NADH dehydrogenase  [3] (data from MRSA252)
    SACOL0973fumarylacetoacetate hydrolase  [3] (data from MRSA252)
    SACOL1437CSD family cold shock protein  [3] (data from MRSA252)
    SACOL1753universal stress protein  [3] (data from MRSA252)
    SACOL1759universal stress protein  [3] (data from MRSA252)
    SACOL1792hypothetical protein  [3] (data from MRSA252)
    SACOL1801dipeptidase PepV  [3] (data from MRSA252)
    SACOL1952ferritins family protein  [3] (data from MRSA252)
    SACOL2072DEAD/DEAH box helicase  [3] (data from MRSA252)
    SACOL2131Dps family protein  [3] (data from MRSA252)
    SACOL2553pyruvate oxidase  [3] (data from MRSA252)
    SACOL2609hypothetical protein  [3] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  3. ↑ 3.000 3.001 3.002 3.003 3.004 3.005 3.006 3.007 3.008 3.009 3.010 3.011 3.012 3.013 3.014 3.015 3.016 3.017 3.018 3.019 3.020 3.021 3.022 3.023 3.024 3.025 3.026 3.027 3.028 3.029 3.030 3.031 3.032 3.033 3.034 3.035 3.036 3.037 3.038 3.039 3.040 3.041 3.042 3.043 3.044 3.045 3.046 3.047 3.048 3.049 3.050 3.051 3.052 3.053 3.054 3.055 3.056 3.057 3.058 3.059 3.060 3.061 3.062 3.063 3.064 3.065 3.066 3.067 3.068 3.069 3.070 3.071 3.072 3.073 3.074 3.075 3.076 3.077 3.078 3.079 3.080 3.081 3.082 3.083 3.084 3.085 3.086 3.087 3.088 3.089 3.090 3.091 3.092 3.093 3.094 3.095 3.096 3.097 3.098 3.099 3.100 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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