From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0617 [new locus tag: SACOL_RS03190 ]
  • pan locus tag?: SAUPAN002347000
  • symbol: SACOL0617
  • pan gene symbol?: hxlA
  • synonym:
  • product: hexulose-6-phosphate synthase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0617 [new locus tag: SACOL_RS03190 ]
  • symbol: SACOL0617
  • product: hexulose-6-phosphate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 650358..650990
  • length: 633
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    GTGGAATTACAATTAGCAATTGATTTATTAAACAAAGAAGACGCGGCTGAGTTAGCAAAT
    AAAGTAAAAGATTATGTAGATATCGTAGAAATCGGTACGCCAATCATTTACAACGAAGGT
    TTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATG
    AAAATTATGGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTA
    ATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCAT
    AAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCA
    AAAGAACTAGATGAAATGGGTGCTGATTATATTGCAGTACACACTGGTTATGATTTACAA
    GCAGAAGGGCAATCACCATTAGAAAGTTTAAGAACCGTTAAATCTGTTATTAAAAATTCT
    AAAGTTGCAGTAGCAGGTGGAATTAAACCAGATACAATTAAAGATATTGTCGCTGAAAGT
    CCTGATCTTGTTATTGTTGGTGGCGGAATCGCAAATGCAGATGATCCAGTAGAAGCTGCA
    AAACAATGTCGCGCTGCAATCGAAGGTAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    633

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0617 [new locus tag: SACOL_RS03190 ]
  • symbol: SACOL0617
  • description: hexulose-6-phosphate synthase
  • length: 210
  • theoretical pI: 4.3222
  • theoretical MW: 22435.5
  • GRAVY: -0.0185714

⊟Function[edit | edit source]

  • reaction:
    EC 4.1.2.43?  ExPASy
    3-hexulose-6-phosphate synthase D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde
  • TIGRFAM:
    3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 297.6)
    and 4 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 55.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 26.5)
    Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 21.6)
    phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 11.2)
  • TheSEED  :
    • D-arabino-3-hexulose 6-phosphate formaldehyde-lyase (EC 4.1.2.43)
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  D-arabino-3-hexulose 6-phosphate formaldehyde-lyase (EC 4.1.2.43)
  • PFAM:
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 104.9)
    and 6 more
    Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 20.2)
    TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 15.3)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 15.1)
    PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 14.9)
    PcrB; PcrB family (PF01884; HMM-score: 13.9)
    ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00232
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000488
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAVKHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKNNKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNSKVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 2206 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigB* (activation) regulon
    SigB*(sigma factor)controlling a large regulon involved in stress/starvation response and adaptation;  [6]   other strains

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 30.59 h [7]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Jan Pané-Farré, Beate Jonas, Konrad Förstner, Susanne Engelmann, Michael Hecker
    The sigmaB regulon in Staphylococcus aureus and its regulation.
    Int J Med Microbiol: 2006, 296(4-5);237-58
    [PubMed:16644280] [WorldCat.org] [DOI] (P p)
  7. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SACOL0617&oldid=88897"