PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0555 [new locus tag: SAUSA300_RS02960 ]
• pan locus tag^?: SAUPAN002347000
• symbol: SAUSA300_0555
• pan gene symbol^?: hxlA
• synonym:
• product: putative hexulose-6-phosphate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0555 [new locus tag: SAUSA300_RS02960 ]
• symbol: SAUSA300_0555
• product: putative hexulose-6-phosphate synthase
• replicon: chromosome
• strand: +
• coordinates: 628890..629522
• length: 633
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915363 NCBI
• RefSeq: YP_493258 NCBI
• BioCyc: see SAUSA300_RS02960
• MicrobesOnline: 1292070 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGGAATTACAATTAGCAATTGATTTATTAAACAAAGAAGACGCGGCTGAGTTAGCAAAT
  AAAGTAAAAGATTATGTAGATATCGTAGAAATCGGTACGCCAATCATTTACAACGAAGGT
  TTACCAGCAGTTAAACATATGGCAGACAACATTAGTAATGTAAAAGTATTAGCAGACATG
  AAAATTATAGATGCAGCTGATTATGAAGTTAGCCAAGCAATTAAATTTGGCGCGGATGTA
  ATTACAATACTAGGTGTTGCAGAAGATGCATCAATTAAAGCAGCTATTGAAGAAGCTCAT
  AAAAATAATAAACAATTACTAGTTGATATGATTGCTGTTCAAGATTTAGAAAAACGTGCA
  AAAGAACTAGATGAAATGGGTGCTGATTATATTGCAGTACACACTGGTTATGATTTACAA
  GCAGAAGGGCAATCACCATTAGAAAGTTTAAGAACCGTTAAATCTGTTATTAAAAATTCT
  AAAGTTGCAGTAGCAGGTGGAATTAAACCAGATACAATTAAAGATATTGTCGCTGAAAGT
  CCTGATCTTGTTATTGTTGGTGGCGGAATCGCAAATGCAGATGATCCAGTAGAAGCTGCA
  AAACAATGTCGCGCTGCAATCGAAGGTAAGTAA
  60
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  633
  

⊟Protein

⊟General

• locus tag: SAUSA300_0555 [new locus tag: SAUSA300_RS02960 ]
• symbol: SAUSA300_0555
• description: putative hexulose-6-phosphate synthase
• length: 210
• theoretical pI: 4.3222
• theoretical MW: 22417.5
• GRAVY: -0.00619048

⊟Function

• reaction:
  EC 4.1.2.43?  ExPASy
  3-hexulose-6-phosphate synthase D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde
• TIGRFAM:
  3-hexulose-6-phosphate synthase (TIGR03128; EC 4.1.2.43; HMM-score: 294.7)
  and 4 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotidine 5'-phosphate decarboxylase (TIGR01740; EC 4.1.1.23; HMM-score: 55.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate diphosphorylase (TIGR00693; EC 2.5.1.3; HMM-score: 26.1)
  Metabolism Energy metabolism Pentose phosphate pathway ribulose-phosphate 3-epimerase (TIGR01163; EC 5.1.3.1; HMM-score: 21.4)
  phosphoglycerol geranylgeranyltransferase (TIGR01769; EC 2.5.1.41; HMM-score: 11.4)
• TheSEED  :
  • D-arabino-3-hexulose 6-phosphate formaldehyde-lyase (EC 4.1.2.43)
  Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  D-arabino-3-hexulose 6-phosphate formaldehyde-lyase (EC 4.1.2.43)
• PFAM:
  TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 104)
  and 6 more
  Ribul_P_3_epim; Ribulose-phosphate 3 epimerase family (PF00834; HMM-score: 20.6)
  PRAI; N-(5'phosphoribosyl)anthranilate (PRA) isomerase (PF00697; HMM-score: 15.1)
  TMP-TENI; Thiamine monophosphate synthase (PF02581; HMM-score: 15.1)
  His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 14.8)
  PcrB; PcrB family (PF01884; HMM-score: 14.3)
  ThiG; Thiazole biosynthesis protein ThiG (PF05690; HMM-score: 10)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002341
  • TAT(Tat/SPI): 0.0001
  • LIPO(Sec/SPII): 0.0005
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160968 NCBI
• RefSeq: YP_493258 NCBI
• UniProt: Q2FJ70 UniProt

⊟Protein sequence

• MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAVKHMADNISNVKVLADMKIIDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKNNKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNSKVAVAGGIKPDTIKDIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0554 > SAUSA300_0555 > SAUSA300_0556

⊟Regulation

• data available for COL, NCTC8325, Newman

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications