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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1816 [new locus tag: SACOL_RS09310 ]
  • pan locus tag?: SAUPAN004454000
  • symbol: putA
  • pan gene symbol?: putA
  • synonym:
  • product: proline dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1816 [new locus tag: SACOL_RS09310 ]
  • symbol: putA
  • product: proline dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 1871486..1872487
  • length: 1002
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCACTATTAAAGAATTTTTTTATCGGATTATCTAATAATAGTTTTTTAAACAACGCA
    GCAAAAAAAGTGGGCCCACGTTTGGGCGCCAATAAAGTCGTTGCCGGAAATACAATTCCA
    GAGTTAATTAATACAATCGAATACTTAAATGACAAGAATATCGCTGTTACGGTAGACAAT
    TTAGGGGAATTTGTCGGTACAGTTGAAGAAAGTAATCATGCTAAAGAACAAATTTTAACA
    ATTATGGACGCGCTTCATCAACATGGCGTAAAGGCACATATGTCTGTTAAATTGAGTCAG
    TTAGGTGCAGAATTCGACTTAGAATTAGCTTACCAAAATTTAAGAGAGATTTTACTTAAA
    GCAAATACTTACAACAATATGCATATAAATATTGATACTGAAAAATATGCTAGCCTGCAA
    CAAATTGTTCAAGTTTTAGATCGCTTAAAAGGCGAATTTAGAAATGTTGGTACTGTAATT
    CAAGCATATTTATACGATAGCCACGAATTAGTTGATAAGTACCAAGATTTACGATTACGT
    TTGGTTAAAGGTGCATATAAAGAAAACGAATCAATTGCATTTCAATCTAAGGAAGACGTA
    GATGCAAATTACATCAAAATAATTGAACAACGTTTGTTAAACGCACGCAATTTCACTTCA
    ATTGCAACACATGACCATCGCATCATTAATCATGTAAAACAATTTATGAAAGAAAATCAC
    ATTGAAAAAGATCGTATGGAATTCCAAATGCTCTATGGTTTTAGATCAGAGTTAGCAGAA
    GAAATCGCAAATGAAGGCTATAATTTCACTATTTATGTACCTTATGGCGATGATTGGTTT
    GCGTATTTTATGAGAAGATTAGCAGAACGCCCACAAAACCTATCTCTTGCTGTAAAAGAA
    TTTGTGAAACCTGCTGGCTTAAAACGTGTTGGCATAATTGCAGCTTTAGGAGCTACAGTT
    ATGTTAGGTTTAAGTACAATTAAAAAATTATGCCGTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1002

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1816 [new locus tag: SACOL_RS09310 ]
  • symbol: PutA
  • description: proline dehydrogenase
  • length: 333
  • theoretical pI: 8.36497
  • theoretical MW: 38029.5
  • GRAVY: -0.244144

⊟Function[edit | edit source]

  • reaction:
    EC 1.5.99.8?  ExPASy
    Transferred entry: 1.5.5.2
  • TIGRFAM:
    acidobacterial duplicated orphan permease (TIGR03434; HMM-score: 15.5)
  • TheSEED  :
    • Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase)
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase)
    and 1 more
    Respiration Electron donating reactions Respiratory dehydrogenases 1  Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase)
  • PFAM:
    FAD_oxidored (CL0086) Pro_dh; Proline dehydrogenase (PF01619; HMM-score: 109.5)
    and 3 more
    no clan defined PorB; Alpha helical Porin B (PF11565; HMM-score: 13.7)
    DUF3347; Protein of unknown function (DUF3347) (PF11827; HMM-score: 13)
    ExbD; Biopolymer transport protein ExbD/TolR (PF02472; HMM-score: 12.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.015058
    • TAT(Tat/SPI): 0.002094
    • LIPO(Sec/SPII): 0.001817
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELINTIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLGAEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVIQAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNFTSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYGDDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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