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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1637 [new locus tag: SACOL_RS08345 ]
  • pan locus tag?: SAUPAN004163000
  • symbol: dnaK
  • pan gene symbol?: dnaK
  • synonym:
  • product: molecular chaperone DnaK

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1637 [new locus tag: SACOL_RS08345 ]
  • symbol: dnaK
  • product: molecular chaperone DnaK
  • replicon: chromosome
  • strand: -
  • coordinates: 1666574..1668406
  • length: 1833
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAGTAAAATTATTGGTATAGACTTAGGTACAACAAATTCATGTGTAACAGTATTAGAA
    GGCGATGAGCCAAAAGTAATTCAAAACCCTGAAGGTTCACGTACAACACCATCTGTTGTA
    GCTTTCAAAAATGGAGAAACTCAAGTTGGTGAAGTAGCAAAACGTCAAGCTATTACAAAC
    CCAAACACTGTTCAATCTATTAAACGTCATATGGGTACTGATTATAAAGTAGATATTGAA
    GGTAAATCATACACACCACAAGAAATCTCAGCTATGATTTTACAAAACTTAAAAAATACA
    GCTGAAAGCTATTTAGGTGAGAAAGTTGACAAAGCTGTAATTACAGTACCTGCATACTTT
    AACGATGCTGAACGTCAAGCAACTAAAGATGCTGGTAAAATTGCTGGTTTAGAAGTTGAG
    CGTATCATTAATGAACCAACAGCTGCAGCATTAGCATATGGTTTAGACAAAACTGATAAA
    GATGAAAAAGTTCTTGTTTTTGACTTAGGTGGCGGTACATTTGACGTATCTATCCTAGAA
    TTAGGTGACGGTGTATTCGAAGTACTATCAACAGCCGGTGACAACAAACTTGGCGGTGAT
    GATTTTGACCAAGTAATTATTGACTACCTAGTTGCAGAATTCAAAAAAGAAAATGGCGTA
    GACTTATCTCAAGATAAAATGGCATTACAACGTTTGAAAGATGCTGCTGAAAAAGCTAAA
    AAAGACTTATCAGGTGTATCACAAACTCAAATCTCATTACCATTTATCTCAGCTGGTGAA
    AACGGTCCATTACACTTAGAAGTAAACTTAACTCGTTCTAAATTTGAAGAATTATCAGAT
    TCATTAATTAGAAGAACAATGGAACCTACACGCCAAGCAATGAAAGACGCTGGCTTAACA
    AACTCAGATATCGATGAAGTTATCTTAGTTGGTGGATCAACTCGTATTCCAGCAGTACAA
    GAAGCTGTCAAAAAAGAAATCGGTAAAGAGCCTAACAAAGGAGTAAACCCGGACGAAGTA
    GTGGCAATGGGAGCTGCAATCCAAGGTGGCGTTATCACAGGTGACGTTAAAGACGTAGTA
    TTATTAGACGTAACACCACTATCTTTAGGTATTGAAATTTTAGGTGGACGTATGAATACG
    TTAATTGAACGTAACACTACGATTCCTACATCTAAATCTCAAATCTATTCAACAGCAGTA
    GATAATCAACCATCAGTAGATGTACACGTATTACAAGGTGAACGTCCAATGGCTGCGGAT
    AATAAAACACTTGGTAGATTCCAATTGACTGATATTCCACCAGCTGAACGTGGTAAACCT
    CAAATTGAAGTAACGTTTGATATCGATAAAAACGGTATTGTAAATGTAACTGCAAAAGAC
    TTAGGTACAAATAAAGAACAAAGAATTACAATTCAATCAAGTTCTTCATTATCAGACGAA
    GAAATCGACCGTATGGTAAAAGATGCTGAAGTTAACGCTGAAGCAGATAAAAAACGTCGT
    GAAGAAGTAGACTTAAGAAACGAAGCTGACAGTCTAGTATTCCAAGTTGAAAAAACTTTA
    ACTGATTTAGGCGAAAATATCGGTGAAGAAGATAAAAAATCTGCTGAAGAGAAAAAAGAC
    GCTCTTAAAACTGCTTTAGAAGGTCAAGATATAGAAGATATTAAATCTAAAAAAGAAGAA
    CTTGAAAAAGTGATTCAAGAATTATCAGCAAAAGTATATGAGCAAGCGGCTCAACAGCAA
    CAACAAGCACAAGGTGCTAATGCTGGTCAAAACAATGATAGTACTGTAGAAGATGCTGAA
    TTTAAAGAAGTAAAAGACGACGACAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1833

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1637 [new locus tag: SACOL_RS08345 ]
  • symbol: DnaK
  • description: molecular chaperone DnaK
  • length: 610
  • theoretical pI: 4.38499
  • theoretical MW: 66360.9
  • GRAVY: -0.523279

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization chaperone protein DnaK (TIGR02350; HMM-score: 938.7)
    and 4 more
    Genetic information processing Protein fate Protein folding and stabilization Fe-S protein assembly chaperone HscA (TIGR01991; HMM-score: 574.4)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan cell shape determining protein, MreB/Mrl family (TIGR00904; HMM-score: 66.9)
    ethanolamine utilization protein EutJ family protein (TIGR02529; HMM-score: 59.7)
    Metabolism Central intermediary metabolism Phosphorus compounds exopolyphosphatase (TIGR03706; EC 3.6.1.11; HMM-score: 13)
  • TheSEED  :
    • Chaperone protein DnaK
    Protein Metabolism Protein folding Protein chaperones  Chaperone protein DnaK
    and 1 more
    Stress Response Heat shock Heat shock dnaK gene cluster extended  Chaperone protein DnaK
  • PFAM:
    Actin_ATPase (CL0108) HSP70; Hsp70 protein (PF00012; HMM-score: 822.8)
    and 5 more
    MreB_Mbl; MreB/Mbl protein (PF06723; HMM-score: 72.4)
    Hydantoinase_A; Hydantoinase/oxoprolinase (PF01968; HMM-score: 16.2)
    StbA; StbA protein (PF06406; HMM-score: 15.7)
    FtsA; Cell division protein FtsA (PF14450; HMM-score: 10.5)
    no clan defined DUF775; Protein of unknown function (DUF775) (PF05603; HMM-score: 7.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.01916
    • TAT(Tat/SPI): 0.001154
    • LIPO(Sec/SPII): 0.025443
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKNGETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAESYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDKDEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKENGVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFEELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKGVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSKSQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDKNGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNEADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQELSAKVYEQAAQQQQQAQGANAGQNNDSTVEDAEFKEVKDDDKK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 6195 [5]
  • interaction partners:
    SACOL1638(grpE)heat shock protein GrpE  [6] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HrcA (repression) regulon
    HrcA(TF)important in Heat shock response; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 32.19 h [8]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  6. ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  7. ↑ Arnaud Chastanet, Juliette Fert, Tarek Msadek
    Comparative genomics reveal novel heat shock regulatory mechanisms in Staphylococcus aureus and other Gram-positive bacteria.
    Mol Microbiol: 2003, 47(4);1061-73
    [PubMed:12581359] [WorldCat.org] [DOI] (P p)
  8. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Vineet K Singh, Sugunya Utaida, Letitia S Jackson, R K Jayaswal, Brian J Wilkinson, Neal R Chamberlain
Role for dnaK locus in tolerance of multiple stresses in Staphylococcus aureus.
Microbiology (Reading): 2007, 153(Pt 9);3162-3173
[PubMed:17768259] [WorldCat.org] [DOI] (P p)

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