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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • pan locus tag?: SAUPAN004114000
  • symbol: gcvT
  • pan gene symbol?: gcvT
  • synonym:
  • product: glycine cleavage system aminomethyltransferase T

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • symbol: gcvT
  • product: glycine cleavage system aminomethyltransferase T
  • replicon: chromosome
  • strand: -
  • coordinates: 1628778..1629869
  • length: 1092
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGTCAAGTGATTTAAAACAAACACCTTTATATCAAAATTATGTTGATAGAGGTGCAAAA
    ATTGTGGAATTCGGAGGATGGGCGATGCCTGTTCAATTTTCAAGTATTAAAGAGGAGCAT
    AATGCTGTTCGATACGAAATTGGCCTGTTTGATGTTAGTCATATGGGTGAAATTGAAGTA
    ACAGGTAAAGATGCTAGTCAGTTTGTGCAATATTTATTATCAAATGATACTGATAATTTA
    ACTACTTCAAAAGCATTATATACTGCTTTATGTAATGAAGAAGGCGGTATTATTGATGAT
    TTAGTAATATATAAATTAGCTGACGACAATTATTTATTAGTTGTTAATGCTGCTAATACT
    GAAAAAGATTTTAATTGGATTTTAAAACACAAAGAGAAATTTGATGTTGAAGTACAAAAT
    GTATCAAACCAATATGGTCAATTAGCAATACAAGGACCAAAAGCTAGAGATTTAATTAAT
    CAATTAGTTGATGAAGATGTAACTGAAATGAAAATGTTTGAATTTAAACAGGGTGTCAAA
    TTATTTGGAGCAAACGTCATTTTATCTCAGTCAGGTTACACAGGTGAAGATGGGTTTGAA
    ATTTATTGTAATATCGATGATACTGAAAAAATTTGGGATGGTTTATTAGAGTATAATGTT
    ATGCCATGTGGGTTAGGCGCTCGTGATACATTAAGATTAGAGGCTGGATTGCCATTACAT
    GGACAAGATTTAACTGAATCAATTACACCATATGAAGGTGGTATCGCTTTTGCAAGTAAA
    CCATTAATTGATGCTGATTTTATTGGTAAATCTGTATTAAAAGATCAAAAAGAAAATGGT
    GCACCAAGAAGAACAGTGGGATTAGAATTACTTGAAAAAGGAATTGCAAGAACTGGTTAT
    GAAGTTATGGATTTAGATGGAAATATTATTGGAGAAGTAACTTCAGGAACACAGTCTCCA
    TCATCAGGAAAATCAATTGCACTTGCAATGATAAAAAGAGATGAGTTTGAAATGGGTAGA
    GAGTTGCTTGTTCAAGTTCGTAAGCGTCAATTAAAAGCGAAAATTGTTAAGAAAAATCAA
    ATTGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1092

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1595 [new locus tag: SACOL_RS08125 ]
  • symbol: GcvT
  • description: glycine cleavage system aminomethyltransferase T
  • length: 363
  • theoretical pI: 4.54011
  • theoretical MW: 40457.6
  • GRAVY: -0.331956

⊟Function[edit | edit source]

  • reaction:
    EC 2.1.2.10?  ExPASy
    Aminomethyltransferase [Protein]-S8-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines glycine cleavage system T protein (TIGR00528; EC 2.1.2.10; HMM-score: 442)
    and 2 more
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, alpha subunit family (TIGR01372; HMM-score: 187.7)
    Metabolism Energy metabolism Amino acids and amines sarcosine oxidase, gamma subunit family (TIGR01375; EC 1.5.3.1; HMM-score: 22)
  • TheSEED  :
    • Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
    and 1 more
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine cleavage system  Aminomethyltransferase (glycine cleavage system T protein) (EC 2.1.2.10)
  • PFAM:
    FBD (CL0289) GCV_T; Aminomethyltransferase folate-binding domain (PF01571; HMM-score: 317.8)
    and 4 more
    GCV_T_C (CL0740) GCV_T_C; Glycine cleavage T-protein C-terminal barrel domain (PF08669; HMM-score: 69.8)
    PRC-barrel (CL0350) PRC; PRC-barrel domain (PF05239; HMM-score: 13.7)
    Hybrid (CL0105) GTP_EFTU_D4; Elongation factor Tu domain 4 (PF14578; HMM-score: 12.5)
    Tubby_C (CL0395) DUF3659; Protein of unknown function (DUF3659) (PF12396; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9952
    • Cytoplasmic Membrane Score: 0.0006
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0042
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0045
    • TAT(Tat/SPI): 0.000579
    • LIPO(Sec/SPII): 0.000703
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVRYEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGGIIDDLVIYKLADDNYLLVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKARDLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDGLLEYNVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSVLKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAMIKRDEFEMGRELLVQVRKRQLKAKIVKKNQIDK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 201 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Gly-box (transcription antitermination) regulon
    Gly-box(RNA)important in Glycine metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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