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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01276
  • pan locus tag?: SAUPAN003612000
  • symbol: glpK
  • pan gene symbol?: glpK
  • synonym:
  • product: glycerol kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01276
  • symbol: glpK
  • product: glycerol kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 1233281..1234777
  • length: 1497
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAAAAATATATTTTATCTATAGACCAAGGAACAACAAGCTCAAGAGCGATTTTATTC
    AATCAAAAAGGGGAAATTGCAGGGGTAGCACAACGTGAGTTTAAGCAATATTTTCCACAA
    TCAGGTTGGGTTGAACATGATGCAAATGAAATTTGGACATCTGTGTTAGCTGTAATGACG
    GAAGTAATTAATGAAAATGATGTTAGAGCTGATCAAATTGCAGGTATCGGTATTACAAAC
    CAACGTGAAACAACGGTTGTTTGGGACAAACATACTGGCCGCCCAATTTATCACGCAATT
    GTTTGGCAATCACGTCAAACACAATCAATTTGTTCAGAATTAAAACAACAAGGATATGAA
    CAAACATTTAGAGATAAGACAGGATTACTTTTAGATCCGTATTTTGCAGGTACAAAAGTT
    AAATGGATTCTAGACAATGTTGAAGGTGCACGAGAAAAAGCAGAAAATGGCGATCTATTA
    TTTGGAACGATTGATACTTGGTTAGTATGGAAATTATCAGGAAAAGCTGCGCATATTACT
    GATTATTCAAATGCGAGTCGTACATTAATGTTTAATATCCATGATTTAGAATGGGACGAT
    GAGTTATTAGAACTACTTACAGTACCTAAAAATATGTTGCCAGAAGTTAAAGCTTCGAGT
    GAAGTATATGGTAAGACAATTGATTACCACTTCTATGGTCAAGAAGTACCAATCGCTGGA
    GTAGCTGGTGATCAACAAGCAGCATTATTTGGACAAGCTTGCTTCGAACGTGGTGACGTG
    AAAAACACATATGGAACTGGTGGCTTCATGTTAATGAATACAGGTGACAAAGCGGTTAAA
    TCTGAAAGTGGTTTATTAACAACAATTGCTTATGGTATTGATGGAAAAGTAAATTATGCG
    CTTGAAGGTTCCATCTTTGTTTCGGGTTCAGCAATCCAATGGTTACGTGATGGATTAAGA
    ATGATTAATTCAGCACCACAATCAGAAAGTTATGCGACACGAGTTGACTCTACTGAGGGT
    GTTTATGTTGTTCCAGCTTTTGTAGGTTTAGGAACACCATATTGGGATTCTGAAGCACGT
    GGTGCGATTTTCGGTTTAACACGTGGAACTGAAAAAGAGCACTTTATCCGTGCAACTTTA
    GAATCACTATGTTACCAAACTCGTGACGTTATGGAAGCAATGTCAAAAGACTCTGGTATT
    GATGTCCAAAGTTTACGTGTCGATGGTGGTGCAGTTAAAAATAACTTTATTATGCAGTTC
    CAAGCAGACATTGTTAATACTTCTGTTGAAAGACCTGAAATTCAAGAAACTACAGCTTTA
    GGTGCTGCATTTTTGGCAGGTTTAGCAGTTGGATTCTGGGAGAGTAAAGATGATATCGCT
    AAAAACTGGAAATTAGAAGAAAAATTCGATCCGAAAATGGATGAAGGCGAAAGAGAAAAA
    TTATATAGAGGTTGGAAAAAAGCTGTTGAAGCAACACAAGTTTTTAAAACAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

Protein Data Bank: 3G25
Protein Data Bank: 3GE1

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01276
  • symbol: GlpK
  • description: glycerol kinase
  • length: 498
  • theoretical pI: 4.6683
  • theoretical MW: 55625.2
  • GRAVY: -0.340964

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.30?  ExPASy
    Glycerol kinase ATP + glycerol = ADP + sn-glycerol 3-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Other glycerol kinase (TIGR01311; EC 2.7.1.30; HMM-score: 760.2)
    and 7 more
    Metabolism Energy metabolism Sugars xylulokinase (TIGR01312; EC 2.7.1.17; HMM-score: 240.7)
    Metabolism Energy metabolism Sugars gluconate kinase (TIGR01314; EC 2.7.1.12; HMM-score: 173.5)
    Metabolism Energy metabolism Sugars L-fuculokinase (TIGR02628; EC 2.7.1.51; HMM-score: 120.9)
    FGGY-family pentulose kinase (TIGR01315; EC 2.7.1.-; HMM-score: 109.4)
    Metabolism Energy metabolism Sugars ribulokinase (TIGR01234; EC 2.7.1.16; HMM-score: 68.5)
    rhamnulokinase (TIGR02627; EC 2.7.1.5; HMM-score: 57.7)
    TraF-like protein (TIGR02740; HMM-score: 12.8)
  • TheSEED  :
    • Glycerol kinase (EC 2.7.1.30)
    Carbohydrates Central carbohydrate metabolism Dihydroxyacetone kinases  Glycerol kinase (EC 2.7.1.30)
    and 2 more
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Glycerol kinase (EC 2.7.1.30)
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerol kinase (EC 2.7.1.30)
  • PFAM:
    Actin_ATPase (CL0108) FGGY_N; FGGY family of carbohydrate kinases, N-terminal domain (PF00370; HMM-score: 296.5)
    and 2 more
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 215.8)
    BcrAD_BadFG; BadF/BadG/BcrA/BcrD ATPase family (PF01869; HMM-score: 19.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004055
    • TAT(Tat/SPI): 0.000391
    • LIPO(Sec/SPII): 0.000446
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWVEHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVWQSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLLFGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKASSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGDKAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATRVDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVMEAMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAVGFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.000 3.001 3.002 3.003 3.004 3.005 3.006 3.007 3.008 3.009 3.010 3.011 3.012 3.013 3.014 3.015 3.016 3.017 3.018 3.019 3.020 3.021 3.022 3.023 3.024 3.025 3.026 3.027 3.028 3.029 3.030 3.031 3.032 3.033 3.034 3.035 3.036 3.037 3.038 3.039 3.040 3.041 3.042 3.043 3.044 3.045 3.046 3.047 3.048 3.049 3.050 3.051 3.052 3.053 3.054 3.055 3.056 3.057 3.058 3.059 3.060 3.061 3.062 3.063 3.064 3.065 3.066 3.067 3.068 3.069 3.070 3.071 3.072 3.073 3.074 3.075 3.076 3.077 3.078 3.079 3.080 3.081 3.082 3.083 3.084 3.085 3.086 3.087 3.088 3.089 3.090 3.091 3.092 3.093 3.094 3.095 3.096 3.097 3.098 3.099 3.100 3.101 3.102 3.103 3.104 3.105 3.106 3.107 3.108 3.109 3.110 3.111 3.112 3.113 3.114 3.115 3.116 3.117 3.118 3.119 3.120 3.121 3.122 3.123 3.124 3.125 3.126 3.127 3.128 3.129 3.130 3.131 3.132 3.133 3.134 3.135 3.136 3.137 3.138 3.139 3.140 3.141 3.142 3.143 3.144 3.145 3.146 3.147 3.148 3.149 3.150 3.151 3.152 3.153 3.154 3.155 3.156 3.157 3.158 3.159 3.160 3.161 3.162 3.163 3.164 3.165 3.166 3.167 3.168 3.169 3.170 3.171 3.172 3.173 3.174 3.175 3.176 3.177 3.178 3.179 3.180 3.181 3.182 3.183 3.184 3.185 3.186 3.187 3.188 3.189 3.190 3.191 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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