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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00532
  • pan locus tag?: SAUPAN002322000
  • symbol: SAOUHSC_00532
  • pan gene symbol?: kbl
  • synonym:
  • product: 2-amino-3-ketobutyrate coenzyme A ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00532
  • symbol: SAOUHSC_00532
  • product: 2-amino-3-ketobutyrate coenzyme A ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 536142..537329
  • length: 1188
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGGTTCAATCATTACATGAGTTTTTAGAGGAAAATATAAATTATCTAAAAGAAAATGGT
    TTGTATAATGAAATAGATACAATTGAAGGTGCAAACGGACCAGAAATCAAAATCAATGGG
    AAATCATACATTAACTTATCTTCAAATAATTATTTAGGACTAGCAACAAATGAAGATTTG
    AAATCAGCTGCAAAAGCAGCTATTGATACACATGGTGTAGGTGCAGGCGCTGTTCGTACA
    ATCAATGGTACATTAGATTTACACGACGAATTAGAAGAAACACTAGCAAAATTTAAAGGA
    ACAGAAGCTGCAATAGCTTATCAATCAGGATTTAATTGTAATATGGCTGCTATTTCAGCT
    GTCATGAATAAAAATGATGCTATTTTATCAGATGAGCTTAATCATGCATCAATTATTGAT
    GGATGTCGCTTATCTAAAGCTAAAATTATTCGAGTTAACCATTCAGACATGGATGATTTA
    CGTGCGAAAGCAAAAGAAGCAGTTGAATCAGGTCAATACAATAAAGTGATGTATATCACT
    GATGGCGTTTTTAGTATGGATGGTGATGTGGCTAAATTACCTGAAATTGTAGAAATTGCA
    GAAGAATTTGGTTTATTAACTTATGTTGACGACGCTCATGGTTCAGGTGTTATGGGTAAA
    GGCGCTGGTACGGTTAAACATTTTGGTTTACAAGATAAAATCGATTTCCAAATAGGTACG
    CTTTCTAAAGCAATTGGTGTCGTTGGCGGTTATGTAGCAGGTACAAAAGAGTTAATAGAT
    TGGTTAAAAGCACAATCACGACCATTCTTATTCTCTACATCATTAGCACCTGGGGATACC
    AAAGCAATAACTGAAGCAGTTAAAAAGTTAATGGATTCAACTGAATTACATGATAAATTA
    TGGAACAATGCACAATATTTAAAAAATGGATTGTCAAAATTAGGATATGATACAGGTGAG
    TCAGAAACTCCAATTACACCAGTAATTATTGGTGATGAAAAAACAACTCAAGAATTTAGT
    AAGCGTTTAAAAGACGAAGGTGTCTATGTGAAATCTATCGTTTTCCCAACAGTACCAAGA
    GGTACAGGACGTGTAAGAAATATGCCTACAGCTGCACATACAAAAGACATGTTAGATGAA
    GCAATTGCGGCTTATGAAAAAGTAGGAAAAGAAATGAAGTTGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00532
  • symbol: SAOUHSC_00532
  • description: 2-amino-3-ketobutyrate coenzyme A ligase
  • length: 395
  • theoretical pI: 5.00874
  • theoretical MW: 42890.3
  • GRAVY: -0.268354

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.29?  ExPASy
    Glycine C-acetyltransferase Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate
  • TIGRFAM:
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 692.5)
    and 13 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 403.6)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 386.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 285.7)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 27.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 22.9)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 19.8)
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 19.8)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.4)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 15.3)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 13.7)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 12.2)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 11.4)
  • TheSEED  :
    • 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
    and 2 more
    Amino Acids and Derivatives Alanine, serine, and glycine Glycine Biosynthesis  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
    Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine degradation  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 233.6)
    and 4 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 27.7)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 18.5)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 18.4)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00477
    • TAT(Tat/SPI): 0.000428
    • LIPO(Sec/SPII): 0.000672
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYINLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTEAAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDLRAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVMGKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEMKLI

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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