PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0535 [new locus tag: SAUSA300_RS02860 ]
• pan locus tag^?: SAUPAN002322000
• symbol: SAUSA300_0535
• pan gene symbol^?: kbl
• synonym:
• product: 2-amino-3-ketobutyrate coenzyme A ligase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0535 [new locus tag: SAUSA300_RS02860 ]
• symbol: SAUSA300_0535
• product: 2-amino-3-ketobutyrate coenzyme A ligase
• replicon: chromosome
• strand: +
• coordinates: 599537..600724
• length: 1188
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914622 NCBI
• RefSeq: YP_493238 NCBI
• BioCyc: see SAUSA300_RS02860
• MicrobesOnline: 1292050 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  GTGGTTCAATCATTACATGAGTTTTTAGAGGAAAATATAAATTATCTAAAAGAAAATGGT
  TTGTATAATGAAATAGATACAATTGAAGGTGCAAACGGACCAGAAATCAAAATCAATGGG
  AAATCATACATTAACTTATCTTCAAATAATTATTTAGGACTAGCAACAAATGAAGATTTG
  AAATCAGCTGCAAAAGCAGCTATTGATACACATGGTGTAGGTGCAGGCGCTGTTCGTACA
  ATCAATGGTACATTAGATTTACACGACGAATTAGAAGAAACACTAGCAAAATTTAAAGGA
  ACAGAAGCTGCAATAGCTTATCAATCAGGATTTAATTGTAATATGGCTGCTATTTCAGCT
  GTCATGAATAAAAATGATGCTATTTTATCAGATGAGCTTAATCATGCATCAATTATTGAT
  GGATGTCGCTTATCTAAAGCTAAAATTATTCGAGTTAACCATTCAGACATGGATGATTTA
  CGTGCGAAAGCAAAAGAAGCAGTTGAATCAGGTCAATACAATAAAGTGATGTATATCACT
  GATGGCGTTTTTAGTATGGATGGTGATGTGGCTAAATTACCTGAAATTGTAGAAATTGCA
  GAAGAATTTGGTTTATTAACTTATGTTGACGACGCTCATGGTTCAGGTGTTATGGGTAAA
  GGCGCTGGTACGGTTAAACATTTTGGTTTACAAGATAAAATCGATTTCCAAATAGGTACG
  CTTTCTAAAGCAATTGGTGTCGTTGGCGGTTATGTAGCAGGTACAAAAGAGTTAATAGAT
  TGGTTAAAAGCACAATCACGACCATTCTTATTCTCTACATCATTAGCACCTGGGGATACC
  AAAGCAATAACTGAAGCAGTTAAAAAGTTAATGGATTCAACTGAATTACATGATAAATTA
  TGGAACAATGCACAATATTTAAAAAATGGATTGTCAAAATTAGGATATGATACAGGTGAG
  TCAGAAACTCCAATTACACCAGTAATTATTGGTGATGAAAAAACAACTCAAGAATTTAGT
  AAGCGTTTAAAAGACGAAGGTGTCTATGTGAAATCTATCGTTTTCCCAACAGTACCAAGA
  GGTACAGGACGTGTAAGAAATATGCCTACAGCTGCACATACAAAAGACATGTTAGATGAA
  GCAATTGCGGCTTATGAAAAAGTAGGAAAAGAAATGAAGTTGATTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0535 [new locus tag: SAUSA300_RS02860 ]
• symbol: SAUSA300_0535
• description: 2-amino-3-ketobutyrate coenzyme A ligase
• length: 395
• theoretical pI: 5.00874
• theoretical MW: 42890.3
• GRAVY: -0.268354

⊟Function

• reaction:
  EC 2.3.1.29?  ExPASy
  Glycine C-acetyltransferase Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate
• TIGRFAM:
  putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 692.5)
  and 13 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 403.6)
  Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 386.6)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 285.7)
  Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 27.2)
  Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 22.9)
  Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 19.8)
  Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 19.8)
  Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.4)
  Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 15.3)
  Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 13.7)
  O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 12.2)
  cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 11.4)
• TheSEED  :
  • 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine and Serine Utilization  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
  and 2 more
  Amino Acids and Derivatives Alanine, serine, and glycine Glycine Biosynthesis  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
  Amino Acids and Derivatives Lysine, threonine, methionine, and cysteine Threonine degradation  2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29)
• PFAM:
  PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 233.6)
  and 4 more
  Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 27.7)
  DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 18.5)
  Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 18.4)
  no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 13.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: pyridoxal 5'-phosphate
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00477
  • TAT(Tat/SPI): 0.000428
  • LIPO(Sec/SPII): 0.000672
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162079 NCBI
• RefSeq: YP_493238 NCBI
• UniProt: A0A0H2XJW8 UniProt

⊟Protein sequence

• MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYINLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTEAAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDLRAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVMGKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEAVKKLMDSTELHDKLWNNAQYLKNGLSKLGYDTGESETPITPVIIGDEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEMKLI

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications