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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_000508
  • pan locus tag?: SAUPAN002322000
  • symbol: JSNZ_000508
  • pan gene symbol?: kbl
  • synonym:
  • product: glycine C-acetyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_000508
  • symbol: JSNZ_000508
  • product: glycine C-acetyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 541642..542829
  • length: 1188
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    GTGGTTCAATCATTACATGAGTTTTTAGAGGAAAATATAAATTATCTAAAAGAAAATGGT
    TTGTATAATGAAATAGATACAATTGAAGGTGCAAACGGACCAGAAATCAAAATCAATGGG
    AAATCATACATTAACTTATCTTCAAATAATTATTTAGGACTAGCAACAAATGAAGATTTG
    AAATCAGCTGCAAAAGCAGCTATTGATACACATGGAGTAGGTGCAGGCGCTGTTCGTACA
    ATCAATGGTACATTAGATTTACACGACGAATTAGAAGAAACACTAGCAAAATTTAAAGGA
    ACAGAAGCTGCAATAGCTTATCAATCAGGATTTAATTGTAATATGGCTGCTATTTCAGCT
    GTCATGAATAAAAATGATGCTATTTTATCAGATGAGCTTAATCATGCATCAATTATTGAT
    GGATGTCGCTTATCTAAAGCTAAAATTATTCGAGTTAACCATTCAGACATGGATGATTTA
    CGTGCGAAAGCAAAAGAAGCAGTTGAATCAGGTCAATACAATAAAGTGATGTATATCACT
    GATGGCGTTTTTAGTATGGATGGTGATGTGGCTAAATTACCTGAAATTGTAGAAATTGCA
    GAAGAATTTGGTTTATTAACTTATGTTGACGACGCTCATGGTTCAGGTGTTATGGGTAAA
    GGCGCTGGTACGGTTAAACATTTTGGTTTACAAGATAAAATCGATTTCCAAATAGGTACG
    CTTTCTAAAGCAATTGGTGTCGTTGGCGGCTATGTAGCAGGTACAAAAGAGTTAATAGAT
    TGGTTAAAAGCACAATCACGACCATTCTTATTCTCTACATCATTAGCACCTGGGGATACC
    AAAGCAATAACTGAAGCAGTTAAAAAGTTAATGGATTCAACTGAATTACATGATAAATTA
    TGGGACAATGCACAATATTTAAAAAATGGATTGTCAAAATTAGGATATGATACAGGTGAG
    TCAGAAACTCCAATTACACCAGTAATTATTGGTGATGAAAAAACAACTCAAGAATTTAGT
    AAGCGTTTAAAAGACGAAGGTGTCTATGTGAAATCTATCGTTTTCCCAACAGTACCAAGA
    GGTACAGGACGTGTAAGAAATATGCCTACAGCTGCACATACAAAAGACATGTTAGATGAA
    GCAATTGCGGCTTATGAAAAAGTAGGAAAAGAAATGAAGTTGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1188

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_000508
  • symbol: JSNZ_000508
  • description: glycine C-acetyltransferase
  • length: 395
  • theoretical pI: 4.93475
  • theoretical MW: 42891.3
  • GRAVY: -0.268354

⊟Function[edit | edit source]

  • reaction:
    EC 2.3.1.29?  ExPASy
    Glycine C-acetyltransferase Acetyl-CoA + glycine = CoA + 2-amino-3-oxobutanoate
  • TIGRFAM:
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 693)
    and 13 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin 8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 403.2)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 386.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin 5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 285.7)
    Metabolism Energy metabolism Amino acids and amines methionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 27.2)
    Metabolism Amino acid biosynthesis Histidine family histidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 21.9)
    Metabolism Amino acid biosynthesis Aspartate family O-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 19.8)
    Unknown function Enzymes of unknown specificity pyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 19.5)
    Metabolism Amino acid biosynthesis Aspartate family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.3)
    Metabolism Amino acid biosynthesis Serine family O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 16.3)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other tyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 16)
    Metabolism Amino acid biosynthesis Aspartate family cystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 13.7)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 12.2)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 11.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 225.7)
    and 5 more
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 27.6)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 20.9)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 20.5)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 14.4)
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 13.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9955
    • Cytoplasmic Membrane Score: 0.0001
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0044
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00477
    • TAT(Tat/SPI): 0.000428
    • LIPO(Sec/SPII): 0.000672
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYINLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTEAAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDLRAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVMGKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLAPGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEKTTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEMKLI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA regulon
    CcpA(TF)important in Carbon catabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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