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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00530
- pan locus tag?: SAUPAN002320000
- symbol: SAOUHSC_00530
- pan gene symbol?: tuf
- synonym:
- product: elongation factor Tu
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3920383 NCBI
- RefSeq: YP_499102 NCBI
- BioCyc: G1I0R-500 BioCyc
- MicrobesOnline: 1289012 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1141ATGGCAAAAGAAAAATTCGATCGTTCTAAAGAACATGCCAATATCGGTACTATCGGTCAC
GTTGACCATGGTAAAACAACATTAACAGCAGCAATCGCTACTGTATTAGCAAAAAATGGT
GACTCAGTTGCACAATCATATGACATGATTGACAACGCTCCAGAAGAAAAAGAACGTGGT
ATCACAATCAATACTTCTCACATTGAGTACCAAACTGACAAACGTCACTACGCTCACGTT
GACTGCCCAGGACACGCTGACTACGTTAAAAACATGATCACTGGTGCTGCTCAAATGGAC
GGCGGTATCTTAGTAGTATCTGCTGCTGACGGTCCAATGCCACAAACTCGTGAACACATT
CTTTTATCACGTAACGTTGGTGTACCAGCATTAGTAGTATTCTTAAACAAAGTTGACATG
GTTGACGATGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGTGACTTATTAAGCGAA
TATGACTTCCCAGGTGACGATGTACCTGTAATCGCTGGTTCAGCATTAAAAGCTTTAGAA
GGCGATGCTCAATACGAAGAAAAAATCTTAGAATTAATGGAAGCTGTAGATACTTACATT
CCAACTCCAGAACGTGATTCTGACAAACCATTCATGATGCCAGTTGAGGACGTATTCTCA
ATCACTGGTCGTGGTACTGTTGCTACAGGCCGTGTTGAACGTGGTCAAATCAAAGTTGGT
GAAGAAGTTGAAATCATCGGTTTACATGACACATCTAAAACAACTGTTACAGGTGTTGAA
ATGTTCCGTAAATTATTAGACTACGCTGAAGCTGGTGACAACATTGGTGCATTATTACGT
GGTGTTGCTCGTGAAGACGTACAACGTGGTCAAGTATTAGCTGCTCCTGGTTCAATTACA
CCACATACTGAATTCAAAGCAGAAGTATACGTATTATCAAAAGACGAAGGTGGACGTCAC
ACTCCATTCTTCTCAAACTATCGTCCACAATTCTATTTCCGTACTACTGACGTAACTGGT
GTTGTTCACTTACCAGAAGGTACTGAAATGGTAATGCCTGGTGATAACGTTGAAATGACA
GTAGAATTAATCGCTCCAATCGCGATTGAAGACGGTACTCGTTTCTCAATCCGTGAAGGT
GGACGTACTGTAGGATCAGGCGTTGTTACTGAAATCATTAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00530
- symbol: SAOUHSC_00530
- description: elongation factor Tu
- length: 394
- theoretical pI: 4.48462
- theoretical MW: 43103.4
- GRAVY: -0.250508
⊟Function[edit | edit source]
- reaction: EC 3.6.5.3? ExPASyProtein-synthesizing GTPase GTP + H2O = GDP + phosphate
- TIGRFAM: Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 773.7)and 18 moreProtein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 216.2)Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 184.5)translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 129.4)Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 122.8)Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 122.8)Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 122.8)Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 112.8)Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 82.3)Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 78.1)Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 68.9)Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 53.3)Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 52)Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 44.6)Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 34.7)Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 13.4)Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 13.4)arsenical pump-driving ATPase (TIGR04291; EC 3.6.1.-; HMM-score: 12.5)cell division ATPase MinD (TIGR01969; HMM-score: 12.3)
- TheSEED :
- Translation elongation factor Tu
Protein Metabolism Protein biosynthesis Translation elongation factors bacterial Translation elongation factor Tuand 2 more - PFAM: P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 204.3)and 6 moreno clan defined GTP_EFTU_D3; Elongation factor Tu C-terminal domain (PF03143; HMM-score: 118.6)EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 67.6)P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 21.7)CbiA; CobQ/CobB/MinD/ParA nucleotide binding domain (PF01656; HMM-score: 14.7)cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 13.6)RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.053712
- TAT(Tat/SPI): 0.012475
- LIPO(Sec/SPII): 0.004058
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVAQSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSEYDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDVFSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIGALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFRTTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEIIK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAOUHSC_02254 (groEL) chaperonin GroEL [4] (data from MRSA252) SAOUHSC_00487 (hslO) Hsp33-like chaperonin [4] (data from MRSA252) SAOUHSC_00519 (rplA) 50S ribosomal protein L1 [4] (data from MRSA252) SAOUHSC_02512 (rplC) 50S ribosomal protein L3 [4] (data from MRSA252) SAOUHSC_00521 (rplL) 50S ribosomal protein L7/L12 [4] (data from MRSA252) SAOUHSC_01232 (rpsB) 30S ribosomal protein S2 [4] (data from MRSA252) SAOUHSC_02506 (rpsC) 30S ribosomal protein S3 [4] (data from MRSA252) SAOUHSC_02494 (rpsE) 30S ribosomal protein S5 [4] (data from MRSA252) SAOUHSC_01234 (tsf) elongation factor Ts [4] (data from MRSA252) SAOUHSC_00187 formate acetyltransferase [4] (data from MRSA252) SAOUHSC_00529 elongation factor G [4] (data from MRSA252) SAOUHSC_00679 hypothetical protein [4] (data from MRSA252) SAOUHSC_00878 hypothetical protein [4] (data from MRSA252) SAOUHSC_01043 dihydrolipoamide dehydrogenase [4] (data from MRSA252) SAOUHSC_01451 threonine dehydratase [4] (data from MRSA252) SAOUHSC_01806 pyruvate kinase [4] (data from MRSA252) SAOUHSC_01819 hypothetical protein [4] (data from MRSA252) SAOUHSC_02849 pyruvate oxidase [4] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: rpoB > SAOUHSC_00525 > SAOUHSC_00526 > rpsL > SAOUHSC_00528 > SAOUHSC_00529 > SAOUHSC_00530predicted SigA promoter [5] : S183 > rplJ > rplL > S184 > SAOUHSC_00523 > S185 > rpoB > S186 > SAOUHSC_00525 > SAOUHSC_00526 > S187 > rpsL > SAOUHSC_00528 > S188 > SAOUHSC_00529 > S189 > SAOUHSC_00530predicted SigA promoter [5] : SAOUHSC_00523 > S185 > rpoB > S186 > SAOUHSC_00525 > SAOUHSC_00526 > S187 > rpsL > SAOUHSC_00528 > S188 > SAOUHSC_00529 > S189 > SAOUHSC_00530predicted SigA promoter [5] : S185 > rpoB > S186 > SAOUHSC_00525 > SAOUHSC_00526 > S187 > rpsL > SAOUHSC_00528 > S188 > SAOUHSC_00529 > S189 > SAOUHSC_00530predicted SigA promoter [5] : SAOUHSC_00526 > S187 > rpsL > SAOUHSC_00528 > S188 > SAOUHSC_00529 > S189 > SAOUHSC_00530
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [5]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 4.00 4.01 4.02 4.03 4.04 4.05 4.06 4.07 4.08 4.09 4.10 4.11 4.12 4.13 4.14 4.15 4.16 4.17 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ 5.0 5.1 5.2 5.3 5.4 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)