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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00529
  • pan locus tag?: SAUPAN002319000
  • symbol: SAOUHSC_00529
  • pan gene symbol?: fusA
  • synonym:
  • product: elongation factor G

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00529
  • symbol: SAOUHSC_00529
  • product: elongation factor G
  • replicon: chromosome
  • strand: +
  • coordinates: 531032..533113
  • length: 2082
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGCTAGAGAATTTTCATTAGAAAAAACTCGTAATATCGGTATCATGGCTCACATTGAT
    GCTGGTAAAACGACTACGACTGAACGTATTCTTTATTACACTGGCCGTATCCACAAAATT
    GGTGAAACACACGAAGGTGCTTCACAAATGGACTGGATGGAGCAAGAACAAGACCGTGGT
    ATTACTATCACATCTGCTGCAACAACAGCAGCTTGGGAAGGTCACCGTGTAAACATTATC
    GATACACCTGGACACGTAGACTTCACTGTAGAAGTTGAACGTTCATTACGTGTACTTGAC
    GGAGCAGTTACAGTACTTGATGCACAATCAGGTGTTGAACCTCAAACTGAAACAGTTTGG
    CGTCAGGCTACAACTTATGGTGTTCCACGTATCGTATTTGTAAACAAAATGGACAAATTA
    GGTGCTAACTTCGAATACTCTGTAAGTACATTACATGATCGTTTACAAGCTAACGCTGCT
    CCAATCCAATTACCAATTGGTGCGGAAGACGAATTCGAAGCAATCATTGACTTAGTTGAA
    ATGAAATGTTTCAAATATACAAATGATTTAGGTACTGAAATTGAAGAAATTGAAATTCCT
    GAAGACCACTTAGATAGAGCTGAAGAAGCTCGTGCTAGCTTAATCGAAGCAGTTGCAGAA
    ACTAGCGACGAATTAATGGAAAAATATCTTGGTGACGAAGAAATTTCAGTTTCTGAATTA
    AAAGAAGCTATCCGCCAAGCTACTACTAACGTAGAATTCTACCCAGTACTTTGTGGTACA
    GCTTTCAAAAACAAAGGTGTTCAATTAATGCTTGACGCTGTAATTGATTACTTACCTTCA
    CCACTAGACGTTAAACCAATTATTGGTCACCGTGCTAGCAACCCTGAAGAAGAAGTAATC
    GCGAAAGCAGACGATTCAGCTGAATTCGCTGCATTAGCGTTCAAAGTTATGACTGACCCT
    TATGTTGGTAAATTAACATTCTTCCGTGTGTATTCAGGTACAATGACATCTGGTTCATAC
    GTTAAGAACTCTACTAAAGGTAAACGTGAACGTGTAGGTCGTTTATTACAAATGCACGCT
    AACTCACGTCAAGAAATCGATACTGTATACTCTGGAGATATCGCTGCTGCGGTAGGTCTT
    AAAGATACAGGTACTGGTGATACTTTATGTGGTGAGAAAAATGACATTATCTTGGAATCA
    ATGGAATTCCCAGAGCCAGTTATTCACTTATCAGTAGAGCCAAAATCTAAAGCTGACCAA
    GATAAAATGACTCAAGCTTTAGTTAAATTACAAGAAGAAGACCCAACATTCCATGCACAC
    ACTGACGAAGAAACTGGACAAGTTATCATCGGTGGTATGGGTGAGCTTCACTTAGACATC
    TTAGTAGACCGTATGAAGAAAGAATTCAACGTTGAATGTAACGTAGGTGCTCCAATGGTT
    TCATATCGTGAAACATTCAAATCATCTGCACAAGTTCAAGGTAAATTCTCTCGTCAATCT
    GGTGGTCGTGGTCAATACGGTGATGTTCACATTGAATTCACACCAAACGAAACAGGCGCA
    GGTTTCGAATTCGAAAACGCTATCGTTGGTGGTGTAGTTCCTCGTGAATACATTCCATCA
    GTAGAAGCTGGTCTTAAAGATGCTATGGAAAATGGTGTTTTAGCAGGTTATCCTTTAATT
    GATGTTAAAGCTAAATTATATGATGGTTCATACCATGATGTCGATTCATCTGAAATGGCC
    TTCAAAATTGCTGCATCATTAGCACTTAAAGAAGCTGCTAAAAAATGTGATCCTGTAATC
    TTAGAACCAATGATGAAAGTAACTATTGAAATGCCTGAAGAGTACATGGGTGATATCATG
    GGTGACGTAACATCTCGTCGTGGACGTGTTGATGGTATGGAACCTCGTGGTAATGCACAA
    GTTGTTAATGCTTATGTACCACTTTCAGAAATGTTCGGTTATGCAACATCATTACGTTCA
    AACACTCAAGGTCGCGGTACTTACACTATGTACTTCGATCACTATGCTGAAGTTCCAAAA
    TCAATCGCTGAAGATATTATCAAGAAAAATAAAGGTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2082

⊟Protein[edit | edit source]

Protein Data Bank: 2MZW

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00529
  • symbol: SAOUHSC_00529
  • description: elongation factor G
  • length: 693
  • theoretical pI: 4.51854
  • theoretical MW: 76611.9
  • GRAVY: -0.348341

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Translation factors translation elongation factor G (TIGR00484; HMM-score: 1207)
    and 16 more
    Genetic information processing Protein synthesis Translation factors translation elongation factor aEF-2 (TIGR00490; HMM-score: 349.2)
    Cellular processes Cellular processes Adaptations to atypical conditions GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.1)
    Genetic information processing Protein synthesis Translation factors GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.1)
    Signal transduction Regulatory functions Other GTP-binding protein TypA/BipA (TIGR01394; HMM-score: 246.1)
    Genetic information processing Protein synthesis Translation factors peptide chain release factor 3 (TIGR00503; HMM-score: 206.9)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 190.8)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 146.9)
    Genetic information processing Protein synthesis Translation factors translation elongation factor Tu (TIGR00485; HMM-score: 59.4)
    Genetic information processing Protein synthesis Translation factors translation elongation factor EF-1, subunit alpha (TIGR00483; HMM-score: 58.1)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 52.5)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 39.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfate adenylyltransferase, large subunit (TIGR02034; EC 2.7.7.4; HMM-score: 36.6)
    Genetic information processing Protein synthesis Translation factors translation initiation factor aIF-2 (TIGR00491; HMM-score: 27.5)
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 26.6)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 26.2)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 11.3)
  • TheSEED  :
    • Translation elongation factor G
    Protein Metabolism Protein biosynthesis Translation elongation factor G family  Translation elongation factor G
    and 3 more
    Protein Metabolism Protein biosynthesis Translation elongation factors bacterial  Translation elongation factor G
    Protein Metabolism Protein biosynthesis Universal GTPases  Translation elongation factor G
    Virulence Virulence - no subcategory Mycobacterium virulence operon involved in protein synthesis (SSU ribosomal proteins)  Translation elongation factor G
  • PFAM:
    P-loop_NTPase (CL0023) GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 234.5)
    and 7 more
    S5 (CL0329) EFG_IV; Elongation factor G, domain IV (PF03764; HMM-score: 152.7)
    EF-G_C (CL0437) EFG_II; Elongation Factor G, domain II (PF14492; HMM-score: 113.9)
    EFG_C; Elongation factor G C-terminus (PF00679; HMM-score: 110.3)
    EFTPs (CL0575) GTP_EFTU_D2; Elongation factor Tu domain 2 (PF03144; HMM-score: 54.7)
    EF-G_C (CL0437) RF3_C; Class II release factor RF3, C-terminal domain (PF16658; HMM-score: 35.4)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 21)
    AIG1; AIG1 family (PF04548; HMM-score: 17.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.00882
    • TAT(Tat/SPI): 0.001526
    • LIPO(Sec/SPII): 0.001261
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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