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⊟Summary[edit | edit source]
- pan ID?: SAUPAN002319000
- symbol?: fusA
- synonym:
- description?: elongation factor G
- elongation factor G
- translation elongation factor G
- protein translation elongation factor G (EF-G)
- translational elongation factor G
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 2836512..2838659
- synteny block?: BlockID0016180
- occurrence?: in 97% of 34 strains
fusA : ribosomal translocase elongation factor G [1]
• A crystal structure is available : 8P2G
Elongation factor G (alt: translocase) binds to L7/L12 of the 50S ribosome. Upon peptide bond formation, the 30S and 50S subunits rotate relative to each other and the tRNA ends no longer bind to the same "PTC center site" (e.g. tRNA in the A site on the 30S is now in the P site on the 50S, tRNA in the P site on the 30S is now in the E site on the 50S). These hybrid A/P and P/E positions stimulate GTP hydrolysis in elongation factor G which undergoes a conformational change, inserting a domain that physically displaces the hybrid tRNAs (and thus the mRNA) to the "PTC center site" it occupies on the 50S (i.e. it "pushes" tRNAs from the 30S P site to the 30S E site to match its 50S E site binding). Elongation factor G then dissociates allowing for another round of protein elongation to occur. Antibiotics such as thiostrepton and fusidic acid inhibit Elongation Factor G activity.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0524 (fus)
08BA02176:
C248_0622 (fus)
11819-97:
MS7_0537 (fusA)
6850:
RSAU_000499 (fusA)
71193:
ST398NM01_0622
ECT-R 2:
ECTR2_501 (fusA)
ED133:
SAOV_0582 (fusA)
ED98:
SAAV_0510 (fusA)
HO 5096 0412:
SAEMRSA15_04740 (fus)
JH1:
SaurJH1_0584 (fusA)
JH9:
SaurJH9_0570 (fusA)
JKD6008:
SAA6008_00553 (fus)
JKD6159:
SAA6159_00501 (fus)
JSNZ:
JSNZ_000505 (fusA)
LGA251:
SARLGA251_04830 (fus)
M013:
M013TW_0534
MRSA252:
SAR0552 (fus)
MSHR1132:
SAMSHR1132_04910
MSSA476:
SAS0505 (fusA)
Mu3:
SAHV_0545 (fus)
Mu50:
SAV0547 (fus)
MW2:
MW0502 (fus)
RF122:
SAB0498 (fus)
ST398:
SAPIG0622 (fusA)
T0131:
SAT0131_00600 (hemE)
TCH60:
HMPREF0772_12642 (fusA)
TW20:
SATW20_06170 (fus)
USA300_TCH1516:
—
VC40:
SAVC_02325
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
N315 MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
NCTC8325 MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
Newman MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
USA300_FPR3757 MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETHEGASQMDWMEQEQDRG
************************************************************
COL ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
N315 ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
NCTC8325 ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
Newman ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
USA300_FPR3757 ITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGAVTVLDAQSGVEPQTETVW
************************************************************
COL RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
N315 RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
NCTC8325 RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
Newman RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
USA300_FPR3757 RQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAAPIQLPIGAEDEFEAIIDLVE
************************************************************
COL MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
N315 MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
NCTC8325 MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
Newman MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
USA300_FPR3757 MKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAVAETSDELMEKYLGDEEISVSEL
************************************************************
COL KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
N315 KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
NCTC8325 KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
Newman KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
USA300_FPR3757 KEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVIDYLPSPLDVKPIIGHRASNPEEEVI
************************************************************
COL AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
N315 AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
NCTC8325 AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
Newman AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
USA300_FPR3757 AKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGTMTSGSYVKNSTKGKRERVGRLLQMHA
************************************************************
COL NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
N315 NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
NCTC8325 NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
Newman NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
USA300_FPR3757 NSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGEKNDIILESMEFPEPVIHLSVEPKSKADQ
************************************************************
COL DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
N315 DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
NCTC8325 DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
Newman DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
USA300_FPR3757 DKMTQALVKLQEEDPTFHAHTDEETGQVIIGGMGELHLDILVDRMKKEFNVECNVGAPMV
************************************************************
COL SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
N315 SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
NCTC8325 SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
Newman SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
USA300_FPR3757 SYRETFKSSAQVQGKFSRQSGGRGQYGDVHIEFTPNETGAGFEFENAIVGGVVPREYIPS
************************************************************
COL VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
N315 VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
NCTC8325 VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
Newman VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
USA300_FPR3757 VEAGLKDAMENGVLAGYPLIDVKAKLYDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVI
************************************************************
COL LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRS
N315 LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRS
NCTC8325 LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRS
Newman LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMLGYATSLRS
USA300_FPR3757 LEPMMKVTIEMPEEYMGDIMGDVTSRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRS
***************************************************:********
COL NTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE
N315 NTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE
NCTC8325 NTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE
Newman HTQGRGTYTMYFDHYAEIPKSIAEDIIKKNKGE
USA300_FPR3757 NTQGRGTYTMYFDHYAEVPKSIAEDIIKKNKGE
:****************:***************
- ↑ Silke Besier, Albrecht Ludwig, Volker Brade, Thomas A Wichelhaus
Molecular analysis of fusidic acid resistance in Staphylococcus aureus.
Mol Microbiol: 2003, 47(2);463-9
[PubMed:12519196] [WorldCat.org] [DOI] (P p)Jing-Jing Li, Musturi Venkataramana, Ai-Qing Wang, Suparna Sanyal, Jan-Christer Janson, Zhi-Guo Su
A mild hydrophobic interaction chromatography involving polyethylene glycol immobilized to agarose media refolding recombinant Staphylococcus aureus elongation factor G.
Protein Expr Purif: 2005, 40(2);327-35
[PubMed:15766874] [WorldCat.org] [DOI] (P p)Yang Chen, Ravi Kiran Koripella, Suparna Sanyal, Maria Selmer
Staphylococcus aureus elongation factor G--structure and analysis of a target for fusidic acid.
FEBS J: 2010, 277(18);3789-803
[PubMed:20718859] [WorldCat.org] [DOI] (I p)