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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01801
  • pan locus tag?: SAUPAN004319000
  • symbol: SAOUHSC_01801
  • pan gene symbol?: citC
  • synonym:
  • product: isocitrate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01801
  • symbol: SAOUHSC_01801
  • product: isocitrate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1701494..1702762
  • length: 1269
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACTGCAGAAAAAATTACTCAAGGAACTGAAGGATTAAACGTACCTAATGAACCAATT
    ATCCCATTTATTATCGGTGATGGAATTGGACCGGATATTTGGAAGGCAGCAAGCCGAGTT
    ATAGATGCTGCTGTTGAGAAAGCCTATAATGGCGAAAAACGCATTGAATGGAAAGAAGTG
    CTAGCTGGCCAAAAAGCATTTGATACAACTGGTGAATGGTTACCTCAAGAAACACTTGAT
    ACAATTAAAGAATATTTAATTGCTGTTAAAGGACCTTTAACAACACCAATTGGTGGTGGT
    ATTAGATCATTAAATGTGGCTTTACGCCAAGAATTAGATTTATTTACTTGCTTAAGACCG
    GTACGTTGGTTTAAAGGAGTACCATCACCTGTTAAACGTCCACAAGATGTTGATATGGTT
    ATTTTCCGTGAAAATACTGAAGACATTTATGCTGGTATTGAATTTAAAGAAGGTACAACA
    GAAGTTAAAAAGGTAATTGACTTCTTACAAAACGAAATGGGTGCGACAAACATTCGATTC
    CCAGAAACTTCAGGTATTGGTATTAAACCAGTTTCTAAAGAAGGAACTGAGCGATTAGTT
    AGAGCAGCTATACAATATGCTATCGATAATAACCGTAAATCAGTTACTTTAGTTCATAAA
    GGTAATATTATGAAATTTACAGAAGGCTCATTTAAGCAGTGGGGTTACGATTTAGCATTA
    TCTGAATTTGGTGATCAAGTATTCACTTGGCAACAATATGACGAAATTGTTGAAAATGAA
    GGCAGAGATGCTGCTAATGCTGCTCAAGAAAAAGCTGAAAAAGAAGGCAAGATTATCATT
    AAAGATTCTATTGCTGACATTTTCTTACAACAAATTTTAACTCGTCCAGCTGAGCATGAT
    GTTGTAGCAACTATGAACTTGAATGGTGACTATATTTCAGATGCTTTAGCTGCACAAGTT
    GGTGGTATTGGTATTGCGCCAGGTGCAAACATTAATTATGAAACAGGTCATGCTATTTTT
    GAAGCAACACATGGTACAGCTCCAAAATATGCAGGTTTAAATAAAGTGAATCCATCTTCA
    GTAATTTTAAGTTCTGTATTAATGTTAGAACATTTAGGATGGCAAGAAGCGGCAGATAAG
    ATTACAGATTCAATTGAAGATACAATTGCTTCAAAAGTTGTTACTTATGACTTTGCCCGT
    TTAATGGATGGTGCTGAAGAAGTTTCTACATCAGCATTTGCAGATGAATTGATTAAAAAT
    TTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1269

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01801
  • symbol: SAOUHSC_01801
  • description: isocitrate dehydrogenase
  • length: 422
  • theoretical pI: 4.56927
  • theoretical MW: 46422.3
  • GRAVY: -0.231043

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.41?  ExPASy
    Isocitrate dehydrogenase (NAD+) Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH
    EC 1.1.1.42?  ExPASy
    Isocitrate dehydrogenase (NADP+) Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NADP-dependent (TIGR00183; EC 1.1.1.42; HMM-score: 700)
    and 5 more
    isopropylmalate/isohomocitrate dehydrogenases (TIGR02088; HMM-score: 229.8)
    Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase (TIGR02924; EC 1.1.1.-; HMM-score: 217.2)
    Metabolism Energy metabolism TCA cycle isocitrate dehydrogenase, NAD-dependent (TIGR00175; EC 1.1.1.41; HMM-score: 198.8)
    Metabolism Energy metabolism Other tartrate dehydrogenase (TIGR02089; EC 1.1.1.93; HMM-score: 154.7)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydrogenase (TIGR00169; EC 1.1.1.85; HMM-score: 124.3)
  • TheSEED  :
    • Isocitrate dehydrogenase [NADP] (EC 1.1.1.42)
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Isocitrate dehydrogenase [NADP] (EC 1.1.1.42)
  • PFAM:
    Iso_DH (CL0270) Iso_dh; Isocitrate/isopropylmalate dehydrogenase (PF00180; HMM-score: 404.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, Mn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004062
    • TAT(Tat/SPI): 0.000276
    • LIPO(Sec/SPII): 0.000429
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAAVEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIRSLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTTEVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLVHKGNIMKFTEGSFKQWGYDLALSEFGDQVFTWQQYDEIVENEGRDAANAAQEKAEKEGKIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASKVVTYDFARLMDGAEEVSTSAFADELIKNLK

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • data available for N315

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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