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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01605
  • pan locus tag?: SAUPAN004063000
  • symbol: SAOUHSC_01605
  • pan gene symbol?: gnd
  • synonym:
  • product: 6-phosphogluconate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01605
  • symbol: SAOUHSC_01605
  • product: 6-phosphogluconate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1529679..1531085
  • length: 1407
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGACACAACAAATTGGAGTTATTGGTTTAGCCGTTATGGGTAAAAACCTAGCTTGGAAT
    ATTGAATCACGCGGATATAGTGTATCTGTATTTAACCGCTCAAGTGAAAAAACTGATTTA
    ATGGTTGAAGAATCAAAAGGGAAAAATATTCATCCAACGTATTCATTGGAAGAATTTGTT
    AATTCTTTAGAAAAACCACGTAAAATTTTATTAATGGTTCAAGCAGGTAAAGCTACGGAC
    GCAACGATTGATAGTTTGTTACCTTTATTAGATGATGGAGATATTTTAATTGATGGTGGT
    AATACAAACTATCAAGATACTATCAGACGTAATAAAGCATTAGCACAAAGTGCAATCAAC
    TTTATTGGTATGGGCGTTTCTGGTGGTGAAATTGGAGCGTTAACTGGTCCGTCATTAATG
    CCGGGTGGACAAGAAGAAGCTTATAACAAAGTTGCAGATATTCTTGATGCAATTGCTGCA
    AAGGCAAAAGACGGTGCTTCATGTGTTACTTATATTGGACCTAATGGTGCTGGACACTAT
    GTGAAAATGGTACACAATGGTATCGAATATGCTGACATGCAATTAATTGCAGAAAGTTAT
    GCTATGATGAAAGAATTATTAGGCATGTCTCATGAAGACATTGCTCAAACGTTCAAAGAC
    TGGAATGCTGGTGAATTAGAAAGTTATTTAATTGAAATCACTGGTGATATTTTCATGAAA
    TTAGATGAAAATAAAGAAGCATTAGTTGAAAAAATTCTTGATACTGCAGGTCAAAAAGGT
    ACAGGAAAATGGACTTCAATTAACGCATTAGAATTAGGTATTCCATTAACAATCATTACT
    GAATCTGTATTTGCACGTTTTATCTCTTCAATCAAAGAAGAGCGCGTAAATGCTTCTAAA
    GAATTAAATGGTCCAAAAGCATCATTTGATGGTGACAAGAAAGACTTTTTAGAAAAAATT
    CGTAAAGCATTATATATGAGTAAAATTTGTTCTTATGCACAAGGTTTCGCGCAAATGCGT
    AAAGCAAGTGAAGATAATGAATGGAATTTAAAACTTGGTGATTTAGCTATGATTTGGAGA
    GAAGGTTGCATTATCCGTGCACAATTCTTACAAAAGATCAAAGATGCTTACGATAATAAT
    CCAGGATTACAAAACTTATTGCTAGATCCTTACTTTAAAAACATCGTTACTGAATATCAA
    GATGCGTTACGTGATGTTGTCGCAACTGGTGTTCAAAATGGTGTACCAACACCTGGTTTC
    TCATCAAGTATTAATTACTATGATAGTTACCGTGCTGCTGATTTACCTGCAAACTTAATT
    CAAGCACAACGTGACTACTTCGGTGCTCATACGTACGAAAGAAAAGATAAAGAAGGCGTA
    TTCCATACACAATGGATTGAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1407

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01605
  • symbol: SAOUHSC_01605
  • description: 6-phosphogluconate dehydrogenase
  • length: 468
  • theoretical pI: 4.75082
  • theoretical MW: 51802.4
  • GRAVY: -0.300214

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.44?  ExPASy
    Phosphogluconate dehydrogenase (NADP+-dependent, decarboxylating) 6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH
  • TIGRFAM:
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00873; EC 1.1.1.44; HMM-score: 733.9)
    and 4 more
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 229.9)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 56)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 49.7)
    Metabolism Central intermediary metabolism Phosphorus compounds polyphosphate:AMP phosphotransferase (TIGR03708; EC 2.7.4.-; HMM-score: 12.8)
  • TheSEED  :
    • 6-phosphogluconate dehydrogenase [decarboxylating] (EC 1.1.1.44)
    Carbohydrates Central carbohydrate metabolism Pentose phosphate pathway  6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)
    and 2 more
    Carbohydrates Monosaccharides D-gluconate and ketogluconates metabolism  6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  6-phosphogluconate dehydrogenase, decarboxylating (EC 1.1.1.44)
  • PFAM:
    6PGD_C (CL0106) 6PGD; 6-phosphogluconate dehydrogenase, C-terminal domain (PF00393; HMM-score: 446.4)
    and 5 more
    NADP_Rossmann (CL0063) NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 173.9)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 19)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 16.4)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 14.2)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.0299
    • TAT(Tat/SPI): 0.001479
    • LIPO(Sec/SPII): 0.007243
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEESKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNTNYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAAKAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTFKDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPLTIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIEE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [4] (data from MRSA252)
    SAOUHSC_02477(rpsI)30S ribosomal protein S9  [4] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [4] (data from MRSA252)
    SAOUHSC_01490DNA-binding protein HU  [4] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 4.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 5.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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