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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02869
  • pan locus tag?: SAUPAN006217000
  • symbol: SAOUHSC_02869
  • pan gene symbol?: rocA
  • synonym:
  • product: 1-pyrroline-5-carboxylate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02869
  • symbol: SAOUHSC_02869
  • product: 1-pyrroline-5-carboxylate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2642178..2643722
  • length: 1545
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGTAGTAGAATTTAAAAATGAACCTGGTTACGATTTTTCAGTCCAAGAAAATGTGGAT
    ATGTTTAAGAAAGCATTGAAAGACGTGGAAAAAGAATTAGGTCAAGACATACCATTAGTG
    ATTAATGGAGAGAAGATTTTCAAAGATGACAAAATTAAATCAATCAATCCTGCAGACACA
    TCACAAGTGATTGCAAATGCATCAAAGGCAACAAAACAAGATGTTGAAGATGCGTTTAAA
    GCAGCAAACGAAGCTTATAAATCTTGGAAAACATGGTCAGCAAATGATCGTGCAGAGCTT
    ATGTTACGTGTTTCGGCAATTATTCGTCGTCGAAAAGCGGAAATTGCAGCAATTATGGTT
    TATGAAGCAGGTAAGCCATGGGATGAAGCAGTTGGTGATGCAGCTGAAGGTATTGATTTT
    ATTGAATACTATGCACGTTCAATGATGGACTTGGCTCAAGGTAAGCCAGTACTTGATCGT
    GAAGGTGAACACAATAAATATTTCTATAAATCAATTGGTACAGGTGTTACAATTCCGCCG
    TGGAACTTCCCGTTTGCGATTATGGCTGGTACAACTTTAGCACCAGTTGTGGCAGGTAAT
    ACAGTGTTATTGAAACCTGCTGAGGATACACCTTATATCGCTTATAAATTAATGGAAATT
    TTAGAAGAAGCAGGATTACCAAAAGGCGTAGTTAACTTTGTTCCTGGTGATCCGAAAGAA
    ATTGGTGATTACTTAGTTGATCATAAAGATACGCATTTCGTTACATTTACAGGATCACGT
    GCGACAGGTACTAGAATTTATGAACGTAGTGCCGTTGTTCAAGAAGGTCAAAACTTCTTG
    AAACGTGTTATCGCTGAAATGGGTGGTAAAGATGCCATTGTAGTTGATGAAAATATTGAT
    ACAGATATGGCAGCAGAAGCTATTGTAACGTCAGCATTTGGTTTTTCTGGTCAAAAATGT
    TCAGCATGTTCACGTGCAATTGTGCATAAAGACGTTTATGACGAAGTATTAGAGAAATCA
    ATCAAATTAACAAAAGAATTAACGTTGGGCAATACTGTAGACAATACGTATATGGGCCCA
    GTTATTAATAAAAAACAATTTGATAAGATTAAAAATTATATTGAGATTGGTAAAGAAGAA
    GGTAAATTAGAACAAGGTGGCGGCACTGATGATTCAAAAGGTTACTTTGTAGAACCAACA
    ATCATTTCAGGACTTAAATCTAAAGATAGAATCATGCAAGAAGAAATTTTTGGTCCAGTT
    GTTGGCTTTGTGAAAGTTAACGATTTTGATGAAGCAATTGAGGTTGCAAATGATACAGAT
    TACGGTTTAACTGGTGCAGTGATTACAAATAATCGCGAGCATTGGATTAAAGCGGTAAAC
    GAATTTGATGTAGGCAATTTATACCTTAACAGAGGCTGTACTTCAGCAGTTGTTGGATAC
    CATCCATTTGGTGGTTTCAAAATGTCAGGTACGGACGCTAAAACAGGTAGTCCAGACTAC
    TTATTACACTTCTTAGAACAAAAGGTTGTTTCAGAAATGTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1545

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02869
  • symbol: SAOUHSC_02869
  • description: 1-pyrroline-5-carboxylate dehydrogenase
  • length: 514
  • theoretical pI: 4.723
  • theoretical MW: 56867.1
  • GRAVY: -0.290467

⊟Function[edit | edit source]

  • reaction:
    EC 1.2.1.88?  ExPASy
    L-glutamate gamma-semialdehyde dehydrogenase L-glutamate 5-semialdehyde + NAD+ + H2O = L-glutamate + NADH
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 747.3)
    and 14 more
    Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 403)
    Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 338)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 327.6)
    Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 308.5)
    Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 306.8)
    Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 262.2)
    Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 258.1)
    Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 254.5)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 241.4)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 241.2)
    Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 239.5)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 121.6)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 51.6)
    Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 20.5)
  • TheSEED  :
    • L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88)
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)
    and 1 more
    Amino Acids and Derivatives Proline and 4-hydroxyproline Proline, 4-hydroxyproline uptake and utilization  Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 520.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002866
    • TAT(Tat/SPI): 0.000603
    • LIPO(Sec/SPII): 0.000552
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGEKIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELMLRVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDREGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDSKGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITNNREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQKVVSEMF

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CcpA* regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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