PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002534
• pan locus tag^?: SAUPAN006217000
• symbol: pruA
• pan gene symbol^?: rocA
• synonym:
• product: L-glutamate gamma-semialdehyde dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002534
• symbol: pruA
• product: L-glutamate gamma-semialdehyde dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 2541278..2542822
• length: 1545
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  
  ATGGTAGTAGAATTTAAAAATGAACCTGGTTACGATTTTTCAGTCCAAGAAAATGTGGAT
  ATGTTTAAGAAAGCATTGAAAGACGTGGAAAAAGAATTAGGTCAAGACATACCATTAGTG
  ATTAATGGAGAGAAGATTTTCAAAGATGACAAAATTAAATCAATCAATCCTGCAGACACA
  TCACAAGTGATTGCAAATGCATCAAAGGCAACAAAACAAGATGTTGAAGATGCGTTTAAA
  GCAGCAAACGAAGCTTATAAATCTTGGAAAACATGGTCAGCAAATGATCGTGCAGAGCTT
  ATGTTACGTGTTTCGGCAATTATTCGTCGTCGAAAAGCGGAAATTGCAGCAATTATGGTT
  TATGAAGCAGGTAAGCCATGGGATGAAGCAGTTGGTGATGCAGCTGAAGGTATTGATTTT
  ATTGAATACTATGCACGTTCAATGATGGACTTGGCTCAAGGCAAGCCAGTACTTGATCGT
  GAAGGTGAACACAATAAATATTTCTATAAATCAATTGGTACAGGTGTTACAATTCCGCCG
  TGGAACTTCCCGTTTGCGATTATGGCTGGTACAACTTTAGCACCAGTTGTGGCAGGTAAT
  ACAGTGTTATTGAAACCTGCTGAGGATACACCTTATATCGCTTATAAATTAATGGAAATT
  TTAGAAGAAGCAGGATTACCAAAAGGCGTAGTTAACTTTGTTCCTGGTGATCCGAAAGAA
  ATTGGTGATTACTTAGTTGATCATAAAGATACGCATTTCGTTACATTTACAGGATCACGT
  GCGACAGGTACTAGAATTTATGAACGTAGTGCCGTAGTTCAAGAAGGTCAAAACTTCTTG
  AAACGTGTTATCGCTGAAATGGGTGGTAAAGATGCCATTGTAGTTGATGAAAATATTGAT
  ACAGATATGGCGGCAGAAGCTATTGTAACGTCAGCGTTTGGTTTCTCTGGTCAAAAATGT
  TCTGCATGTTCACGTGCAATTGTGCATAAAGACGTTTATGACGAAGTATTAGAGAAATCA
  ATCAAATTAACAAAAGAATTAACGTTGGGCAATACTGTAGACAATACGTATATGGGCCCA
  GTTATTAATAAAAAACAATTTGATAAGATTAAAAATTATATTGAGATTGGTAAAGAAGAA
  GGTAAATTAGAACAAGGTGGCGGCACTGATGATTCAAAAGGTTACTTTGTAGAACCAACA
  ATCATTTCAGGACTTAAATCTAAAGATAGAATCATGCAAGAAGAAATTTTTGGTCCAGTT
  GTTGGCTTTGTGAAAGTTAACGATTTTGATGAAGCCATTGAGGTTGCAAATGATACAGAT
  TACGGTTTAACTGGTGCAGTGATTACAAATAATCGCGAGCATTGGATTAAAGCGGTAAAC
  GAATTTGATGTAGGTAATTTATACCTTAACAGAGGTTGTACTTCAGCAGTTGTTGGATAC
  CATCCATTTGGTGGTTTCAAAATGTCAGGTACGGACGCTAAAACAGGTAGCCCAGACTAC
  TTATTACACTTCTTAGAACAAAAGGTTGTTTCAGAAATGTTCTAA
  60
  120
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  240
  300
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  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1545
  

⊟Protein

⊟General

• locus tag: JSNZ_002534
• symbol: PruA
• description: L-glutamate gamma-semialdehyde dehydrogenase
• length: 514
• theoretical pI: 4.723
• theoretical MW: 56867.1
• GRAVY: -0.290467

⊟Function

• reaction:
  EC 1.2.1.88?  ExPASy
  L-glutamate gamma-semialdehyde dehydrogenase L-glutamate 5-semialdehyde + NAD⁺ + H₂O = L-glutamate + NADH
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines putative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 747.3)
  and 14 more
  Metabolism Energy metabolism Amino acids and amines 1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 403)
  Cellular processes Cellular processes Adaptations to atypical conditions betaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 338)
  5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 327.6)
  Metabolism Energy metabolism Amino acids and amines delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 308.5)
  Metabolism Central intermediary metabolism Other succinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 306.8)
  Unknown function Enzymes of unknown specificity aldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 262.2)
  Metabolism Energy metabolism Other 2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 258.1)
  Metabolism Energy metabolism Amino acids and amines methylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 254.5)
  1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 241.4)
  putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 241.2)
  Metabolism Energy metabolism Amino acids and amines succinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 239.5)
  phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 121.6)
  phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 51.6)
  Metabolism Amino acid biosynthesis Glutamate family glutamate-5-semialdehyde dehydrogenase (TIGR00407; EC 1.2.1.41; HMM-score: 20.5)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 509.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.845
  • Cytoplasmic Membrane Score: 0.0266
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.1278
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002866
  • TAT(Tat/SPI): 0.000603
  • LIPO(Sec/SPII): 0.000552
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MVVEFKNEPGYDFSVQENVDMFKKALKDVEKELGQDIPLVINGEKIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELMLRVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDREGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLMEILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEGQNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYDEVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDSKGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITNNREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQKVVSEMF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications