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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01240
- pan locus tag?: SAUPAN003564000
- symbol: SAOUHSC_01240
- pan gene symbol?: proS
- synonym:
- product: prolyl-tRNA synthetase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3920265 NCBI
- RefSeq: YP_499773 NCBI
- BioCyc: G1I0R-1158 BioCyc
- MicrobesOnline: 1289687 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1681ATGAAGCAATCCAAAGTTTTTATACCAACGATGCGTGACGTGCCATCAGAAGCAGAAGCA
CAAAGTCATCGTTTATTATTGAAATCGGGTTTGATAAAACAAAGTACAAGTGGGATTTAT
AGTTATTTACCGCTAGCAACACGTGTGTTAAATAATATTACTGCAATTGTGCGACAAGAA
ATGGAACGTATCGATTCTGTTGAAATTTTAATGCCAGCGTTACAACAAGCTGAATTATGG
GAAGAATCAGGACGTTGGGGTGCATATGGCCCAGAATTAATGCGTTTACAAGATAGACAT
GGAAGACAATTTGCATTAGGTCCAACACATGAAGAATTAGTTACATCAATAGTAAGAAAT
GAATTGAAATCATACAAACAATTACCGATGACATTATTCCAAATTCAATCTAAATTCCGT
GATGAAAAGAGACCACGTTTTGGTTTACTTCGTGGGCGTGAATTTATTATGAAAGATGCA
TATTCATTCCATGCTGACGAGGCATCATTAGATCAAACGTATCAAGATATGTATCAAGCG
TATAGCCGTATTTTTGAGAGAGTTGGCATTAACGCAAGACCAGTAGTTGCAGATTCAGGT
GCTATAGGCGGTAGCCATACACATGAATTTATGGCATTAAGTGCTATCGGTGAGGATACA
ATCGTTTACAGTAAAGAAAGTGATTATGCTGCTAACATCGAAAAAGCAGAAGTCGTTTAC
GAACCAAATCATAAGCATACTACTGTGCAACCTTTAGAAAAAATTGAAACACCAAATGTT
AAGACTGCGCAAGAATTGGCAGACTTCTTAGGTAGACCAGTAGATGAAATCGTTAAAACG
ATGATTTTCAAAGTTGATGGCGAATATATTATGGTTTTAGTGCGTGGCCATCATGAAATT
AATGACATTAAATTAAAATCTTATTTCGGCACAGATAATATTGAATTAGCAACACAAGAC
GAAATTGTTAATTTAGTTGGTGCAAATCCTGGTTCACTAGGTCCTGTAATTGATAAAGAA
ATCAAAATTTATGCAGATAATTTTGTGCAAGATTTAAATAATTTAGTTGTCGGTGCTAAC
GAAGATGGTTATCACTTAATTAATGTAAATGTAGGTAGAGACTTCAACGTTGATGAATAT
GGCGATTTCCGTTTTATTTTAGAAGGCGAAAAGTTAAGTGATGGTTCAGGCGTTGCACAT
TTTGCTGAAGGTATTGAAGTTGGTCAAGTATTCAAATTGGGTACTAAGTATTCAGAATCA
ATGAATGCTACATTCTTAGATAACCAAGGAAAAGCTCAATCTTTAATTATGGGTTGTTAC
GGAATTGGAATTTCTAGAACGCTAAGTGCGATTGTTGAACAAAATCACGATGATAATGGA
ATTGTTTGGCCTAAATCAGTTACTCCGTTTGATTTACATTTAATTTCTATTAATCCTAAG
AAAGATGATCAACGAGAACTAGCAGATGCACTATATGCTGAATTTAATACTAAATTTGAT
GTGTTGTACGATGATCGTCAGGAACGTGCAGGTGTTAAATTTAATGATGCCGATTTAATT
GGTTTACCACTGCGAATTGTTGTTGGTAAACGTGCATCGGAAGGTATTGTAGAAGTTAAA
GAACGTTTAACAGGTGATAGCGAAGAAGTTCACATTGATGACTTAATGACTGTCATTACA
AATAAATATGATAACTTAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01240
- symbol: SAOUHSC_01240
- description: prolyl-tRNA synthetase
- length: 567
- theoretical pI: 4.84812
- theoretical MW: 63862.8
- GRAVY: -0.350088
⊟Function[edit | edit source]
- reaction: EC 6.1.1.15? ExPASyProline--tRNA ligase ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
- TIGRFAM: Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 741.6)and 6 moreProtein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 129.9)Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 79.2)Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 40.3)Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 35.9)Protein synthesis tRNA aminoacylation Cys-tRNA(Pro) deacylase (TIGR00011; EC 3.6.1.-; HMM-score: 35)Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 24.7)
- TheSEED :
- Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type
Prolyl-tRNA synthetase associated editing enzymes Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial typeand 1 more - PFAM: tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 183.4)and 4 moreno clan defined tRNA_edit; Aminoacyl-tRNA editing domain (PF04073; HMM-score: 95.9)IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 61)no clan defined DUF1543; Domain of Unknown Function (DUF1543) (PF07566; HMM-score: 23.2)FlaA; Flagellar filament outer layer protein Flaa (PF04620; HMM-score: 12.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.013355
- TAT(Tat/SPI): 0.002282
- LIPO(Sec/SPII): 0.001072
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLPLATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGRQFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDAYSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGEDTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPLEKIETPNVKTAQELADFLGRPVDEIVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLGPVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDEYGDFRFILEGEKLSDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQSLIMGCYGIGISRTLSAIVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQERAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLMTVITNKYDNLK
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAOUHSC_01794 glyceraldehyde 3-phosphate dehydrogenase 2 [4] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01239 > SAOUHSC_01240predicted SigA promoter [5] : SAOUHSC_01222 > S504 > SAOUHSC_01223 > S505 > SAOUHSC_01224 > SAOUHSC_01225 > hslU > SAOUHSC_01228 > S506 > SAOUHSC_01230 > S507 > S508 > rpsB > SAOUHSC_01233 > tsf > S509 > pyrH > frr > S510 > S511 > SAOUHSC_01237 > SAOUHSC_01238 > S512 > SAOUHSC_01239 > SAOUHSC_01240predicted SigA promoter [5] : S508 > rpsB > SAOUHSC_01233 > tsf > S509 > pyrH > frr > S510 > S511 > SAOUHSC_01237 > SAOUHSC_01238 > S512 > SAOUHSC_01239 > SAOUHSC_01240 > S513 > polC > S514 > S515 > SAOUHSC_01242 > nusA > SAOUHSC_01244 > SAOUHSC_01245 > infBpredicted SigA promoter [5] : S511 > SAOUHSC_01237 > SAOUHSC_01238 > S512 > SAOUHSC_01239 > SAOUHSC_01240
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [5]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p) - ↑ 5.0 5.1 5.2 5.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)