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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01241
  • pan locus tag?: SAUPAN003565000
  • symbol: polC
  • pan gene symbol?: polC
  • synonym:
  • product: DNA polymerase III PolC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01241
  • symbol: polC
  • product: DNA polymerase III PolC
  • replicon: chromosome
  • strand: +
  • coordinates: 1189525..1193835
  • length: 4311
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAATCAATTA
    GATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAAAACAGA
    ACATGGGAATTTCATATTACATTACCACAATTCTTAGCTCATGAAGATTATTTATTATTT
    ATAAATGCAATAGAGCAAGAGTTTAAAGATATCGCCAACGTTACATGTCGTTTTACGGTA
    ACAAATGGCACGAATCAAGATGAACATGCAATTAAATACTTTGGGCACTGTATTGACCAA
    ACAGCTTTATCTCCAAAAGTTAAAGGTCAATTGAAACAGAAAAAGCTTATTATGTCTGGA
    AAAGTATTAAAAGTAATGGTATCAAATGACATTGAACGTAATCATTTTGATAAGGCATGT
    AATGGAAGTCTTATCAAAGCGTTTAGAAATTGTGGTTTTGATATCGATAAAATCATATTC
    GAAACAAATGATAATGATCAAGAACAAAACTTAGCTTCTTTAGAAGCACATATTCAAGAA
    GAAGACGAACAAAGTGCACGATTGGCAACAGAGAAACTTGAAAAAATGAAAGCTGAAAAA
    GCGAAACAACAAGATAACAACGAAAGTGCTGTCGATAAGTGTCAAATTGGTAAGCCGATT
    CAAATTGAAAATATTAAACCAATTGAATCTATTATTGAGGAAGAGTTTAAAGTTGCAATA
    GAGGGTGTCATTTTTGATATAAACTTAAAAGAACTTAAAAGTGGTCGCCATATCGTAGAA
    ATTAAAGTGACTGACTATACGGACTCTTTAGTTTTAAAAATGTTTACTCGTAAAAACAAA
    GATGATTTAGAACATTTTAAAGCGCTAAGTGTTGGTAAATGGGTTAGGGCTCAAGGTCGT
    ATTGAAGAAGATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAAGAGATT
    AAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAGCGTGTAGAATTCCACTTGCATACT
    GCAATGAGCCAAATGGATGGTATACCCAATATTGGTGCGTATGTTAAACAGGCAGCAGAC
    TGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCAGATGCT
    CACGCAGCAGCGGAAAAACATGGCATTAAAATGATATACGGTATGGAAGGTATGTTAGTT
    GATGATGGTGTTCCGATTGCATACAAACCACAAGATGTCGTATTAAAAGATGCTACTTAT
    GTTGTGTTCGACGTTGAGACAACTGGTTTATCAAATCAGTATGATAAAATCATCGAGCTT
    GCAGCTGTGAAAGTTCATAACGGTGAAATCATCGATAAGTTTGAAAGGTTTAGTAATCCG
    CATGAACGATTATCGGAAACGATTATCAATTTGACGCATATTACTGATGATATGTTAGTA
    GATGCCCCTGAGATTGAAGAAGTACTTACAGAGTTTAAAGAATGGGTTGGCGATGCGATA
    TTCGTAGCGCATAATGCTTCGTTTGATATGGGCTTCATCGATACGGGATATGAACGTCTT
    GGGTTTGGACCATCAACGAATGGTGTTATCGATACTTTAGAATTATCTCGTACGATTAAT
    ACTGAATATGGTAAACATGGTTTGAATTTCTTGGCTAAAAAATATGGCGTAGAATTAACG
    CAACATCACCGTGCCATTTATGATACAGAAGCAACAGCTTACATTTTCATAAAAATGGTT
    CAACAAATGAAAGAATTAGGCGTATTAAATCATAACGAAATCAACAAAAAACTCAGTAAT
    GAAGATGCATATAAACGTGCAAGACCTAGTCATGTCACATTAATTGTACAAAACCAACAA
    GGTCTTAAAAATCTATTTAAAATTGTAAGTGCATCATTGGTGAAGTATTTCTACCGTACA
    CCTCGAATTCCACGTTCATTGTTAGATGAATATCGTGAGGGATTATTGGTAGGTACAGCG
    TGTGATGAAGGTGAATTATTTACGGCAGTTATGCAGAAGGACCAGAGTCAAGTTGAAAAA
    ATTGCCAAATATTATGATTTTATTGAAATTCAACCACCGGCACTTTATCAAGATTTAATT
    GATAGAGAGCTTATTAGAGATACTGAAACATTACATGAAATTTATCAACGTTTAATACAT
    GCAGGTGACACAGCGGGTATACCTGTTATTGCGACAGGAAATGCACACTATTTGTTTGAA
    CATGATGGTATCGCACGTAAAATTTTAATAGCATCACAACCCGGCAATCCACTTAATCGC
    TCAACTTTACCGGAAGCACATTTTAGAACTACAGATGAAATGTTAAACGAGTTTCATTTT
    TTAGGTGAAGAAAAAGCGCATGAAATTGTTGTGAAAAATACAAACGAATTAGCAGATCGA
    ATTGAACGTGTTGTTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGTGCTAAC
    GAAGAAATTAGAGAACTAAGTTATGCAAATGCGCGTAAACTGTATGGTGAAGACCTGCCT
    CAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGATTTGCG
    GTAATTTACTTAATTTCGCAACGTTTAGTTAAAAAATCATTAGATGATGGATACTTAGTT
    GGTTCCCGTGGTTCAGTAGGTTCTAGTTTTGTAGCGACAATGACTGAGATTACTGAAGTA
    AACCCGTTACCGCCACACTATATTTGTCCGAACTGTAAAACGAGTGAATTTTTCAATGAT
    GGTTCAGTAGGATCAGGATTTGATTTACCTGATAAGACGTGTGAAACTTGTGGAGCGCCA
    CTTATTAAAGAAGGACAAGATATTCCGTTTGAAACATTTTTAGGATTTAAGGGAGATAAA
    GTTCCTGATATCGACTTAAACTTTAGTGGTGAATATCAACCGAATGCCCATAACTACACA
    AAAGTATTATTTGGTGAGGATAAAGTATTCCGTGCAGGTACAATTGGTACTGTTGCTGAA
    AAGACTGCTTTTGGTTATGTTAAAGGTTATTTGAATGATCAAGGTATCCACAAAAGAGGT
    GCTGAAATAGATCGACTCGTTAAAGGATGTACAGGTGTTAAACGTACAACTGGACAGCAT
    CCAGGGGGTATTATTGTAGTACCTGATTACATGGATATTTATGATTTTACGCCGATACAA
    TATCCTGCCGATGATCAAAATTCAGCATGGATGACGACACATTTTGATTTCCATTCTATT
    CATGATAATGTATTAAAACTTGATATACTTGGACACGATGATCCAACAATGATTCGTATG
    CTTCAAGATTTATCAGGAATTGATCCAAAAACAATACCTGTAGATGATAAAGAAGTTATG
    CAGATATTTAGTACACCTGAAAGTTTGGGTGTTACTGAAGATGAAATTTTATGTAAAACA
    GGTACATTTGGGGTACCAGAATTCGGTACAGGATTCGTGCGTCAAATGTTAGAAGATACA
    AAGCCAACAACATTTTCTGAATTAGTTCAAATCTCAGGATTATCTCATGGTACAGATGTG
    TGGTTAGGCAATGCTCAAGAATTAATTAAAACCGGTATATGTGATTTATCAAGTGTAATT
    GGTTGTCGTGATGATATCATGGTTTATTTAATGTATGCTGGTTTAGAACCATCAATGGCT
    TTTAAAATAATGGAGTCAGTACGTAAAGGTAAAGGTTTAACTGAAGAAATGATTGAAACG
    ATGAAAGAAAATGAAGTGCCAGATTGGTATTTAGATTCATGTCTTAAAATTAAGTACATG
    TTCCCTAAAGCCCATGCAGCAGCATACGTTTTAATGGCAGTACGTATCGCATATTTCAAA
    GTACATCATCCACTTTATTACTATGCATCTTACTTTACAATTCGTGCGTCAGACTTTGAT
    TTAATCACGATGATTAAAGATAAAACAAGCATTCGAAATACTGTAAAAGACATGTATTCT
    CGCTATATGGATCTAGGTAAAAAAGAAAAAGACGTATTAACAGTCTTGGAAATTATGAAT
    GAAATGGCGCATCGAGGTTATCGAATGCAACCGATTAGTTTAGAAAAGAGTCAGGCGTTC
    GAATTTATCATTGAAGGCGATACACTTATTCCGCCGTTCATATCAGTGCCTGGGCTTGGC
    GAAAACGTTGCGAAACGAATTGTTGAAGCTCGTGACGATGGCCCATTTTTATCAAAAGAA
    GATTTAAACAAAAAAGCTGGATTATCTCAGAAAATTATTGAGTATTTAGATGAGTTAGGC
    TCATTACCGAATTTACCAGATAAAGCTCAACTTTCGATATTTGATATGTAA
    60
    120
    180
    240
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    900
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    1560
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    1680
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    1860
    1920
    1980
    2040
    2100
    2160
    2220
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    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4311

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01241
  • symbol: PolC
  • description: DNA polymerase III PolC
  • length: 1436
  • theoretical pI: 5.0377
  • theoretical MW: 162459
  • GRAVY: -0.325905

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 2033.4)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 217.9)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 184.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 149.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 94.7)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 45.4)
  • TheSEED  :
    • DNA polymerase III polC-type (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III alpha subunit (EC 2.7.7.7)
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 331.3)
    and 11 more
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 182.2)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 149.3)
    DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 63.1)
    no clan defined DNA_pol3_a_NI; DNA polymerase III polC-type N-terminus I (PF14480; HMM-score: 59.8)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 37.9)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 36.4)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.6)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 15.4)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 11.2)
    RNase_H (CL0219) CAF1; CAF1 family ribonuclease (PF04857; HMM-score: 5.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000845
    • TAT(Tat/SPI): 0.000099
    • LIPO(Sec/SPII): 0.000185
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEFHITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTALSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIFETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGKPIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFTRKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMIYGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASFDMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAIYDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKNLFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIAKYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFEHDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELADRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIGNGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKTSEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEYQPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGCTGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLDILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVPEFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRDDIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFPKAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYSRYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPGLGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

R Inoue, C Kaito, M Tanabe, K Kamura, N Akimitsu, K Sekimizu
Genetic identification of two distinct DNA polymerases, DnaE and PolC, that are essential for chromosomal DNA replication in Staphylococcus aureus.
Mol Genet Genomics: 2001, 266(4);564-71
[PubMed:11810227] [WorldCat.org] [DOI] (P p)

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