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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1107 [new locus tag: SA_RS06270 ]
  • pan locus tag?: SAUPAN003565000
  • symbol: polC
  • pan gene symbol?: polC
  • synonym:
  • product: DNA polymerase III PolC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1107 [new locus tag: SA_RS06270 ]
  • symbol: polC
  • product: DNA polymerase III PolC
  • replicon: chromosome
  • strand: +
  • coordinates: 1253872..1258188
  • length: 4317
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    TTGGCAATGACAGAGCAACAAAAATTTAAAGTGCTTGCTGATCAAATTAAAATTTCAAAT
    CAATTAGATGCTGAAATTTTAAATTCAGGTGAACTGACACGTATAGATGTTTCTAACAAA
    AACAGAACATGGGAATTTCATATTACATTACCACAATTCTTAGCTCATGAAGATTATTTA
    TTATTTATAAATGCAATAGAGCAAGAGTTTAAAGATATCGCCAACGTTACATGTCGTTTT
    ACGGTAACAAATGGCACGAATCAAGATGAACATGCAATTAAATACTTTGGGCACTGTATT
    GACCAAACAGCTTTATCTCCAAAAGTTAAAGGTCAATTGAAACAGAAAAAGCTTATTATG
    TCTGGAAAAGTATTAAAAGTAATGGTATCAAATGACATTGAACGTAATCATTTTGATAAG
    GCATGTAATGGAAGTCTTATCAAAGCGTTTAGAAATTGTGGTTTTGATATCGATAAAATC
    ATATTCGAAACAAATGATAATGATCAAGAACAAAACTTAGCTTCTTTAGAAGCACATATT
    CAAGAAGAAGACGAACAAAGTGCACGATTGGCAACAGAGAAACTTGAAAAAATGAAAGCT
    GAAAAAGCGAAACAACAAGATAACAACGAAAGTGCTGTCGATAAGTGTCAAATTGGTAAG
    CCGATTCAAATTGAAAATATTAAACCAATTGAATCTATTATTGAGGAAGAGTTTAAAGTT
    GCAATAGAGGGTGTCATTTTTGATATAAACTTAAAAGAACTTAAAAGTGGTCGTCATATC
    GTAGAAATTAAAGTGACTGACTATACGGACTCATTAGTTTTAAAAATGTTTACTCGTAAA
    AACAAAGATGATTTAGAACATTTTAAAGCGCTAAGTGTAGGTAAATGGGTTAGGGCTCAA
    GGTCGTATTGAAGAAGATACATTTATTAGAGATTTAGTTATGATGATGTCTGATATTGAA
    GAGATTAAAAAAGCGACAAAAAAAGATAAGGCTGAAGAAAAGCGTGTAGAATTCCACTTG
    CATACTGCAATGAGCCAAATGGATGGTATACCCAATATTGGTGCGTATGTTAAACAGGCA
    GCAGACTGGGGACATCCAGCCATTGCGGTTACAGACCATAATGTTGTGCAAGCATTTCCA
    GATGCTCACGCAGCAGCGGAAAAACATGGCATTAAAATGATATACGGTATGGAAGGTATG
    TTAGTTGATGATGGTGTTCCGATTGCATACAAACCACAAGATGTCGTATTAAAAGATGCT
    ACTTATGTTGTGTTCGACGTTGAGACAACTGGTTTATCAAATCAGTATGATAAAATCATC
    GAGCTTGCAGCTGTGAAAGTTCATAACGGTGAAATCATCGATAAGTTTGAAAGGTTTAGT
    AATCCGCATGAACGATTATCGGAAACGATTATCAATTTGACGCATATTACTGATGATATG
    TTAGTAGATGCCCCTGAGATTGAAGAAGTACTTACAGAGTTTAAAGAATGGGTTGGCGAT
    GCGATATTCGTAGCGCATAATGCTTCGTTTGATATGGGCTTTATCGATACGGGATATGAA
    CGTCTTGGGTTTGGACCATCAACGAATGGTGTTATCGATACTTTAGAATTATCTCGTACG
    ATTAATACTGAATATGGTAAACATGGTTTGAATTTCTTAGCTAAAAAATATGGCGTAGAA
    TTAACGCAACATCACCGTGCCATTTATGATACAGAAGCAACAGCTTACATTTTCATAAAA
    ATGGTTCAACAAATGAAAGAATTAGGTGTATTAAATCATAACGAAATCAACAAAAAACTC
    AGTAATGAAGATGCATATAAACGTGCAAGACCTAGCCATGTCACATTAATTGTACAAAAC
    CAACAAGGTCTTAAAAATCTATTTAAAATTGTAAGTGCATCATTGGTGAAGTATTTCTAC
    CGTACACCTCGAATTCCACGTTCATTGTTAGATGAATATCGTGAGGGATTATTGGTAGGT
    ACAGCGTGTGATGAAGGTGAATTATTTACGGCAGTTATGCAGAAGGACCAGAGCCAAGTT
    GAAAAAATTGCCAAATATTATGATTTTATTGAAATTCAACCACCGGCACTTTATCAAGAT
    TTAATTGATAGAGAGCTTATTAGAGATACTGAAACATTACATGAAATTTATCAACGTTTA
    ATACATGCAGGTGACACAGCGGGTATACCTGTTATTGCGACAGGAAATGCACACTATTTG
    TTTGAACATGATGGTATCGCACGTAAAATTTTAATAGCATCACAACCCGGCAATCCACTT
    AATCGCTCAACTTTACCGGAAGCACATTTTAGAACTACAGATGAAATGTTAAACGAGTTT
    CATTTTTTAGGTGAAGAAAAAGCGCATGAAATTGTTGTGAAAAATACAAACGAATTAGCA
    GATCGAATTGAACGTGTTGTTCCTATTAAAGATGAATTATACACACCGCGTATGGAAGGT
    GCTAACGAAGAAATTAGAGAACTAAGTTATACAAATGCGCGTAAACTGTATGGTGAAGAC
    CTGCCTCAAATCGTAATTGATCGATTAGAAAAAGAATTAAAAAGTATTATCGGTAATGGA
    TTTGCGGTAATTTACTTAATTTCGCAACGTTTAGTTAAAAAATCATTAGATGATGGATAC
    TTAGTTGGTTCCCGTGGTTCAGTAGGTTCTAGTTTTGTAGCGACAATGACTGAGATTACT
    GAAGTAAACCCGTTACCGCCACACTATATTTGTCCGAACTGTAAAACGAGTGAATTTTTC
    AATGATGGTTCAGTAGGATCAGGATTCGATTTACCTGATAAGACGTGTGAAACTTGTGGA
    GCGCCACTTATTAAAGAAGGACAAGATATTCCGTTTGAAACATTTTTAGGATTTAAGGGA
    GATAAAGTTCCTGATATCGACTTAAACTTTAGTGGTGAATATCAACCGAATGCCCATAAC
    TACACAAAAGTATTATTTGGTGAGGATAAAGTATTCCGTGCAGGTACAATTGGTACCGTT
    GCTGAAAAGACTGCTTTTGGTTATGTTAAAGGTTATTTGAATGATCAAGGCATCCACAAA
    AGAGGCGCTGAAATAGATCGACTCGTTAAAGGATGTACAGGTGTTAAACGTACAACTGGA
    CAGCATCCAGGAGGTATTATTGTAGTACCTGATTACATGGATATTTATGATTTTACGCCG
    ATACAATATCCTGCCGATGATCAAAATTCAGCATGGATGACGACACATTTTGATTTCCAT
    TCTATTCATGATAATGTATTAAAACTTGATATACTTGGACACGATGATCCAACAATGATT
    CGTATGCTTCAAGATTTATCGGGCATTGATCCAAAAACGATACCTGTAGATGACAAAGAA
    GTCATGCAAATATTTAGTACACCTGAAAGTTTAGGTGTTACTGAAGATGAAATTTTATGT
    AAAACAGGTACGTTTGGGGTTCCAGAATTCGGTACAGGGTTCGTGCGTCAAATGTTAGAA
    GATACAAAGCCAACAACATTTTCTGAATTAGTTCAAATCTCAGGATTATCTCATGGTACA
    GATGTGTGGTTAGGCAATGCTCAAGAATTAATTAAAACCGGTATATGTGATTTATCAAGT
    GTAATTGGTTGTCGTGATGATATCATGGTTTATTTAATGTATGCTGGTTTAGAACCATCA
    ATGGCTTTTAAAATAATGGAGTCAGTACGTAAAGGTAAAGGTTTAACTGAAGAAATGATT
    GAAACGATGAAAGAAAATGAAGTGCCGGATTGGTATTTAGATTCATGTCTTAAAATTAAG
    TACATGTTCCCTAAAGCCCATGCAGCAGCATACGTTTTAATGGCAGTACGTATCGCATAT
    TTCAAAGTACATCATCCACTTTATTACTATGCATCTTACTTTACAATTCGTGCGTCAGAT
    TTTGATTTAATCACGATGATTAAAGATAAAACAAGCATTCGAAATACTGTAAAAGACATG
    TATTCTCGCTATATGGATCTAGGTAAAAAAGAAAAAGACGTATTAACAGTCTTGGAAATT
    ATGAATGAAATGGCGCATCGAGGTTATCGAATGCAACCGATTAGTTTAGAAAAGAGTCAG
    GCGTTCGAATTTATCATTGAAGGCGATACACTTATTCCGCCGTTCATATCAGTGCCTGGG
    CTTGGCGAAAACGTTGCGAAACGAATTGTTGAAGCTCGTGACGATGGCCCATTTTTATCA
    AAAGAAGATTTAAACAAAAAAGCTGGATTATCTCAGAAAATTATTGAGTATTTAGATGAG
    TTAGGCTCATTACCGAATTTACCAGATAAAGCTCAACTTTCGATATTTGATATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4317

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1107 [new locus tag: SA_RS06270 ]
  • symbol: PolC
  • description: DNA polymerase III PolC
  • length: 1438
  • theoretical pI: 5.0377
  • theoretical MW: 162692
  • GRAVY: -0.324618

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.7.7?  ExPASy
    DNA-directed DNA polymerase Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit, Gram-positive type (TIGR01405; EC 2.7.7.7; HMM-score: 2033.2)
    and 5 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, alpha subunit (TIGR00594; EC 2.7.7.7; HMM-score: 217.9)
    Genetic information processing DNA metabolism Degradation of DNA exonuclease, DNA polymerase III, epsilon subunit family (TIGR00573; EC 2.7.7.7; HMM-score: 184.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair putative DnaQ family exonuclease/DinG family helicase (TIGR01407; HMM-score: 149.7)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, epsilon subunit (TIGR01406; EC 2.7.7.7; HMM-score: 94.7)
    Genetic information processing Transcription RNA processing ribonuclease T (TIGR01298; EC 3.1.13.-; HMM-score: 45.4)
  • TheSEED  :
    • DNA polymerase III alpha subunit, class II (EC 2.7.7.7)
    DNA Metabolism DNA replication DNA-replication  DNA polymerase III alpha subunit (EC 2.7.7.7)
  • PFAM:
    NTP_transf (CL0260) DNA_pol3_alpha; Bacterial DNA polymerase III alpha subunit (PF07733; HMM-score: 331.7)
    and 11 more
    Amidohydrolase (CL0034) PHP; PHP domain (PF02811; HMM-score: 182.2)
    RNase_H (CL0219) RNase_T; Exonuclease (PF00929; HMM-score: 149.3)
    DnaA_N (CL0494) DNA_pol3_a_NII; DNA polymerase III polC-type N-terminus II (PF11490; HMM-score: 63.1)
    no clan defined DNA_pol3_a_NI; DNA polymerase III polC-type N-terminus I (PF14480; HMM-score: 59.8)
    HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 37.9)
    HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 36.4)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 18.6)
    RNase_H (CL0219) RNase_H_2; RNase_H superfamily (PF13482; HMM-score: 15.4)
    HHH (CL0198) HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 13.4)
    Zn_Beta_Ribbon (CL0167) DZR; Double zinc ribbon (PF12773; HMM-score: 11.2)
    RNase_H (CL0219) CAF1; CAF1 family ribonuclease (PF04857; HMM-score: 5.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.000922
    • TAT(Tat/SPI): 0.000093
    • LIPO(Sec/SPII): 0.000167
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAMTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEFHITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHAIKYFGHCIDQTALSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIFETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGKPIQIENIKPIESIIEEEFKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFTRKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRVEFHLHTAMSQMDGIPNIGAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMIYGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEIIDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASFDMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAIYDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKNLFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIAKYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFEHDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELADRIERVVPIKDELYTPRMEGANEEIRELSYTNARKLYGEDLPQIVIDRLEKELKSIIGNGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKTSEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEYQPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGCTGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLDILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTEDEILCKTGTFGVPEFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRDDIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFPKAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYSRYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPGLGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ R Allyn Forsyth, Robert J Haselbeck, Kari L Ohlsen, Robert T Yamamoto, Howard Xu, John D Trawick, Daniel Wall, Liangsu Wang, Vickie Brown-Driver, Jamie M Froelich, Kedar G C, Paula King, Melissa McCarthy, Cheryl Malone, Brian Misiner, David Robbins, Zehui Tan, Zhan-yang Zhu Zy, Grant Carr, Deborah A Mosca, Carlos Zamudio, J Gordon Foulkes, Judith W Zyskind
    A genome-wide strategy for the identification of essential genes in Staphylococcus aureus.
    Mol Microbiol: 2002, 43(6);1387-400
    [PubMed:11952893] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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