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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01493
  • pan locus tag?: SAUPAN003937000
  • symbol: rpsA
  • pan gene symbol?: rpsA
  • synonym:
  • product: 30S ribosomal protein S1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01493
  • symbol: rpsA
  • product: 30S ribosomal protein S1
  • replicon: chromosome
  • strand: -
  • coordinates: 1448594..1449769
  • length: 1176
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACTGAAGAATTCAATGAATCAATGATTAACGATATTAAAGAAGGTGACAAAGTCACT
    GGCGAGGTACAACAAGTTGAAGACAAGCAAGTTGTTGTTCATATCAACGGTGGTAAATTT
    AATGGGATTATTCCTATTAGTCAACTATCTACGCATCATATTGATAGCCCAAGTGAAGTT
    GTAAAAGAGGGCGACGAAGTTGAAGCATATGTCACTAAAGTTGAGTTTGATGAAGAAAAT
    GAAACTGGAGCTTACATCTTATCTAGAAGACAACTTGAAACTGAGAAGTCTTATAGTTAT
    TTACAAGAAAAATTAGATAATAATGAAATCATCGAAGCGAAAGTAACAGAAGTAGTTAAA
    GGTGGTTTGGTTGTTGATGTAGGACAAAGAGGTTTTGTTCCGGCTTCACTAATTTCAACA
    GACTTCATTGAGGATTTCTCTGTGTTTGATGGACAAACAATTCGTATTAAAGTTGAAGAA
    TTGGATCCTGAAAATAATAGAGTCATTTTAAGCCGTAAAGCAGTTGAACAAGAAGAAAAC
    GATGCTAAAAAAGATCAATTATTACAATCTTTAAATGAAGGCGATGTTATTGATGGTAAA
    GTAGCGCGTTTAACTCAATTTGGTGCATTTATAGACATTGGCGGTGTTGATGGTTTAGTG
    CATGTATCTGAACTTTCTCACGAACATGTTCAAACACCAGAAGAAGTAGTTTCAATTGGT
    CAAGATGTTAAAGTTAAAATTAAATCTATTGATAGAGATACAGAACGTATTTCATTATCA
    ATCAAAGATACGTTACCAACACCTTTCGAAAATATTAAAGGTCAATTCCACGAAAATGAT
    GTCATTGAAGGTGTCGTAGTAAGATTGGCAAACTTTGGTGCATTTGTTGAAATTGCACCA
    GGTGTACAAGGACTTGTACATATTTCTGAAATTGCACACAAACACATTGGTACGCCAGGT
    GAAGTGTTAGAACCTGGTCAACAAGTAAATGTTAAAATATTAGGTATTGATGAAGAGAAT
    GAAAGAGTATCACTATCTATTAAAGCAACATTACCAAACGAAGATGTTGTTGAAAGTGAT
    CCTTCTACGACTAAGGCGTACTTAGAAAACGAAGAAGAAGATAATCCAACAATTGGCGAT
    ATGATTGGTGATAAACTTAAAAATCTTAAACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01493
  • symbol: RpsA
  • description: 30S ribosomal protein S1
  • length: 391
  • theoretical pI: 4.23557
  • theoretical MW: 43287
  • GRAVY: -0.390537

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 286.5)
    and 8 more
    Genetic information processing Transcription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 147.8)
    guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 58.7)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 44.2)
    Genetic information processing Transcription Degradation of RNA ribonuclease R (TIGR02063; EC 3.1.-.-; HMM-score: 36.7)
    MAST domain (TIGR04204; HMM-score: 14.1)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis carbon storage regulator (TIGR00202; HMM-score: 9.8)
    Signal transduction Regulatory functions RNA interactions carbon storage regulator (TIGR00202; HMM-score: 9.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 8.5)
  • TheSEED  :
    • SSU ribosomal protein S1p
    Protein Metabolism Protein biosynthesis Ribosome SSU bacterial  SSU ribosomal protein S1p
  • PFAM:
    OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 252.3)
    and 8 more
    CusF_Ec; Copper binding periplasmic protein CusF (PF11604; HMM-score: 16.6)
    Cdc48_2-like (CL0402) CDC48_2; Cell division protein 48 (CDC48), domain 2 (PF02933; HMM-score: 15.9)
    OB (CL0021) S1_2; S1 domain (PF13509; HMM-score: 15.7)
    EXOSC1; Exosome component EXOSC1/CSL4 (PF10447; HMM-score: 12.4)
    RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 10.5)
    no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 9.9)
    CsrA; Global regulator protein family (PF02599; HMM-score: 7.9)
    KOW (CL0107) KOW; KOW motif (PF00467; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021822
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.000886
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGIIPISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQEKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEELDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDGLVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQFHENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKILGIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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