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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01491
  • pan locus tag?: SAUPAN003935000
  • symbol: gpsA
  • pan gene symbol?: gpsA
  • synonym:
  • product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01491
  • symbol: gpsA
  • product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 1446047..1447045
  • length: 999
  • essential: no [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACTAAAATTACCGTTTTTGGTATGGGAAGTTTTGGGACAGCCCTTGCCAATGTTCTT
    GCAGAAAATGGACATGATGTTTTGATGTGGGGTAAAAATCAAGATGCTGTTGATGAATTA
    AATACATGTCATACAAATAAAAAGTATTTAAAATACGCGAAATTAGATGTTAACATCATC
    GCTACTTCAGATATGACCAAAGCAATTCAATTTGCAGATATTTACTTAATGGCTTTACCT
    ACTAAAGCAATGCGAGAAGTTGCTTCTCAAATTAATGATAAGCTGACCTCTAAAAAGACT
    TTTATACATGTTGCTAAAGGTATTGAAAATGGGACGTTTAAACGTGTGTCAGAAATGATT
    GAAGATTCTATTTCACCTGAATATAATGCAGGTATTGGCGTGTTGTCAGGGCCAAGTCAT
    GCGGAAGAAGTTGTAGTCAAGCAACCAACTACAGTTGCTGCTTCATCAAAAGATAAAAGT
    GTAAGTAAATTAACGCAAGATTTATTTATGAATGATTATTTGCGTGTGTACACGAATGAT
    GACTTGATTGGTGTTGAACTTGGTGGTGCATTGAAAAATATCATCGCAGTAGCAAGTGGT
    ATCGTAGCTGGAATTGGCTACGGTGATAATGCAAAAGCTGCATTAATGACTCGTGGCTTA
    GCGGAAATTAGTAGATTAGGTGAAAAGTTAGGTGCCGATCCTATGACATTTCTAGGTTTA
    GGTGGTATCGGTGACTTAATCGTTACTTGCACATCAACACATTCTCGGAATTTCACATTA
    GGATATAAACTTGGACAAGGTGAATCAATGGATCAAGCATTATCTGAAATGAATATGGTT
    GTTGAAGGTATTTATACAACTAAATCAGTTTATCATTTAGCTAAAGAAAAAAATGTGGAT
    ATGCCAATTACAAATGCATTATATAGAGTATTATTTGAAAATATCTCAGTAAAAGAATGC
    GTAAAAGATTTAATGGAGCGCGATAAAAAATCTGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    999

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01491
  • symbol: GpsA
  • description: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
  • length: 332
  • theoretical pI: 6.26285
  • theoretical MW: 36071.3
  • GRAVY: -0.0858434

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.94?  ExPASy
    Glycerol-3-phosphate dehydrogenase (NAD(P)+) sn-glycerol 3-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H
  • TIGRFAM:
    glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 236.2)
    and 8 more
    nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 40)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 31.2)
    Metabolism Energy metabolism Electron transport NADPH-dependent F420 reductase (TIGR01915; HMM-score: 24.5)
    Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 22.4)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 22)
    3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 15.5)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00873; EC 1.1.1.44; HMM-score: 13.6)
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 13.3)
  • TheSEED  :
    • Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
    Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
    and 2 more
    Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
    Stress Response Oxidative stress Glutaredoxins  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
  • PFAM:
    6PGD_C (CL0106) NAD_Gly3P_dh_C; NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus (PF07479; HMM-score: 192.5)
    NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 175)
    and 12 more
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 42.9)
    UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 35.7)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 33.4)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29.1)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 23.4)
    3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 23.1)
    TrkA_N; TrkA-N domain (PF02254; HMM-score: 17.9)
    2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.4)
    6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 14.9)
    Nucleot_cyclase (CL0276) GCH_III; GTP cyclohydrolase III (PF05165; HMM-score: 14.5)
    NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.8)
    PDH; Prephenate dehydrogenase (PF02153; HMM-score: 12.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: N-terminally anchored (with CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: NGHDVLMW
  • SignalP: Signal peptide SP(Sec/SPI) length 21 aa
    • SP(Sec/SPI): 0.614414
    • TAT(Tat/SPI): 0.005277
    • LIPO(Sec/SPII): 0.014003
    • Cleavage Site: CS pos: 21-22. VLA-EN. Pr: 0.5386
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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