PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1363 [new locus tag: SAUSA300_RS07435 ]
• pan locus tag^?: SAUPAN003935000
• symbol: gpsA
• pan gene symbol^?: gpsA
• synonym:
• product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1363 [new locus tag: SAUSA300_RS07435 ]
• symbol: gpsA
• product: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1529425..1530423
• length: 999
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914986 NCBI
• RefSeq: YP_494060 NCBI
• BioCyc: see SAUSA300_RS07435
• MicrobesOnline: 1292878 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGACTAAAATTACCGTTTTTGGTATGGGAAGTTTTGGGACAGCCCTTGCCAATGTTCTT
  GCAGAAAATGGACATGATGTTTTGATGTGGGGTAAAAATCAAGATGCTGTTGATGAATTA
  AATACATGTCATACAAATAAAAAGTATTTAAAATACGCGAAATTAGATGTTAACATCATC
  GCTACTTCAGATATGACCAAAGCAATTCAATTTGCAGATATTTACTTAATGGCTTTACCT
  ACTAAAGCAATGCGAGAAGTTGCTTCTCAAATTAATGATAAGCTGACCTCTAAAAAGACT
  TTTATACATGTTGCTAAAGGTATTGAAAATGGGACGTTTAAACGTGTGTCAGAAATGATT
  GAAGATTCTATTTCACCTGAATATAATGCAGGTATTGGCGTGTTGTCAGGGCCAAGTCAT
  GCGGAAGAAGTTGTAGTCAAGCAACCAACTACAGTTGCTGCTTCATCAAAAGATAAAAGT
  GTAAGTAAATTAACGCAAGATTTATTTATGAATGATTATTTGCGTGTGTACACGAATGAT
  GACTTGATTGGTGTTGAACTTGGTGGTGCATTGAAAAATATCATCGCAGTAGCAAGTGGT
  ATCGTAGCTGGAATTGGCTACGGTGATAATGCAAAAGCTGCATTAATGACTCGTGGCTTA
  GCGGAAATTAGTAGATTAGGTGAAAAGTTAGGTGCCGATCCTATGACATTTCTAGGTTTA
  GGTGGTATCGGTGACTTAATCGTTACTTGCACATCAACACATTCTCGGAATTTCACATTA
  GGATATAAACTTGGACAAGGTGAATCAATGGATCAAGCATTATCTGAAATGAATATGGTT
  GTTGAAGGTATTTATACAACTAAATCAGTTTATCATTTAGCTAAAGAAAAAAATGTGGAT
  ATGCCAATTACAAATGCATTATATAGAGTATTATTTGAAAATATCTCAGTAAAAGAATGC
  GTAAAAGATTTAATGGAGCGCGATAAAAAATCTGAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  999
  

⊟Protein

⊟General

• locus tag: SAUSA300_1363 [new locus tag: SAUSA300_RS07435 ]
• symbol: GpsA
• description: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
• length: 332
• theoretical pI: 6.26285
• theoretical MW: 36071.3
• GRAVY: -0.0858434

⊟Function

• reaction:
  EC 1.1.1.8?  ExPASy
  Glycerol-3-phosphate dehydrogenase (NAD⁺) sn-glycerol 3-phosphate + NAD⁺ = glycerone phosphate + NADH
  EC 1.1.1.94?  ExPASy
  Glycerol-3-phosphate dehydrogenase (NAD(P)⁺) sn-glycerol 3-phosphate + NAD(P)⁺ = glycerone phosphate + NAD(P)H
• TIGRFAM:
  glycerol-3-phosphate dehydrogenase (NAD(+)) (TIGR03376; EC 1.1.1.8; HMM-score: 236.2)
  and 8 more
  nucleotide sugar dehydrogenase (TIGR03026; HMM-score: 40)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 31.2)
  Metabolism Energy metabolism Electron transport NADPH-dependent F420 reductase (TIGR01915; HMM-score: 24.5)
  Metabolism Amino acid biosynthesis Glutamate family pyrroline-5-carboxylate reductase (TIGR00112; EC 1.5.1.2; HMM-score: 22.4)
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 22)
  3-hydroxyacyl-CoA dehydrogenase PaaC (TIGR02279; EC 1.1.1.-; HMM-score: 15.5)
  Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00873; EC 1.1.1.44; HMM-score: 13.6)
  Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 13.3)
• TheSEED  :
  • Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
  Carbohydrates Sugar alcohols Glycerol and Glycerol-3-phosphate Uptake and Utilization  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
  and 2 more
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
  Stress Response Oxidative stress Glutaredoxins  Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94)
• PFAM:
  6PGD_C (CL0106) NAD_Gly3P_dh_C; NAD-dependent glycerol-3-phosphate dehydrogenase C-terminus (PF07479; HMM-score: 192.5)
  NADP_Rossmann (CL0063) NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 175)
  and 12 more
  F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 42.9)
  UDPG_MGDP_dh_N; UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain (PF03721; HMM-score: 35.7)
  ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 33.4)
  NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 29.1)
  IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 23.4)
  3HCDH_N; 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain (PF02737; HMM-score: 23.1)
  TrkA_N; TrkA-N domain (PF02254; HMM-score: 17.9)
  2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 15.4)
  6PGD_C (CL0106) ApbA_C; Ketopantoate reductase PanE/ApbA C terminal (PF08546; HMM-score: 14.9)
  Nucleot_cyclase (CL0276) GCH_III; GTP cyclohydrolase III (PF05165; HMM-score: 14.5)
  NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 13.8)
  PDH; Prephenate dehydrogenase (PF02153; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: N-terminally anchored (with CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: NGHDVLMW
• SignalP: Signal peptide SP(Sec/SPI) length 21 aa
  • SP(Sec/SPI): 0.614414
  • TAT(Tat/SPI): 0.005277
  • LIPO(Sec/SPII): 0.014003
  • Cleavage Site: CS pos: 21-22. VLA-EN. Pr: 0.5386
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87159940 NCBI
• RefSeq: YP_494060 NCBI
• UniProt: Q2FGW8 UniProt

⊟Protein sequence

• MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: gpsA < engA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications