PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

⊟Summary

• pan ID^?: SAUPAN003935000
• symbol^?: gpsA
• synonym:
• description^?: NAD(P)H-dependent glycerol-3-phosphate dehydrogenase

  descriptions from strain specific annotations:

  • NAD(P)H-dependent glycerol-3-phosphate dehydrogenase
  • glycerol-3-phosphate dehydrogenase (NAD(P)(+))
  • glycerol-3-phosphate dehydrogenase, NAD(P)-dependent
  • Glycerol-3-phosphate dehydrogenase (NAD(P)+)
  • glycerol-3-phosphate dehydrogenase [NAD(P)+]
  • glycerol-3-phosphate dehydrogenase [NAD(P)+] (NAD(P)H-dependent glycerol-3-phosphate dehydrogenase) (NAD(P)H-dependentdihydroxyacetone-phosphate reductase)
  • NAD-dependent glycerol-3-phosphate dehydrogenase family protein
• strand^?: -
• coordinates^?: 4252908..4253906
• synteny block^?: BlockID0029810
• occurrence^?: in 100% of 34 strains

gpsA : glycerol-3-phosphate dehydrogenase

Staphylococci produce two variants of glycerol-3-phosphate dehydrogenase (GPDH) that catalyze the reversible interconversion between sn-glycerol-3-phosphate (GroP) and dihydroxyacetone phosphate (DHAP). GpsA uses either NADH or NADPH as a reducing cofactor and operates under all conditions whereas GlpD uses reduced menaquinones as its reducing cofactor and only functions during aerobic growth. These enzymes localize to the inner membrane and are critical for multiple cellular processes including phospholipid biosynthesis (through GroP generation), glycolysis, gluconeogenesis, redox homeostasis and electron transport.

⊟Orthologs

COL:
SACOL1514 (gpsA)
N315:
SA1306 (gpsA)
NCTC8325:
SAOUHSC_01491 (gpsA)
Newman:
NWMN_1383 (gpsA)
USA300_FPR3757:
SAUSA300_1363 (gpsA)
04-02981:
SA2981_1430 (gpsA)
08BA02176:
C248_1513 (gpsA)
11819-97:
MS7_1428
6850:
RSAU_001344 (gpsA)
71193:
ST398NM01_1472
ECT-R 2:
ECTR2_1326
ED133:
SAOV_1480 (gpsA)
ED98:
SAAV_1461 (gpsA)
HO 5096 0412:
SAEMRSA15_13340 (gpsA)
JH1:
SaurJH1_1561 (gpsA)
JH9:
SaurJH9_1531 (gpsA)
JKD6008:
SAA6008_01442 (gpsA)
JKD6159:
SAA6159_01338 (gpsA)
JSNZ:
JSNZ_001458
LGA251:
SARLGA251_13860 (gpsA)
M013:
M013TW_1421
MRSA252:
SAR1483 (gpsA)
MSHR1132:
SAMSHR1132_13130
MSSA476:
SAS1415 (gpsA)
Mu3:
SAHV_1462 (gpsA)
Mu50:
SAV1474 (gpsA)
MW2:
MW1363 (gpsA)
RF122:
SAB1336c (gpsA)
ST398:
SAPIG1472
T0131:
SAT0131_01562 (set9nm)
TCH60:
HMPREF0772_11732 (gpsA)
TW20:
SATW20_14710 (gpsA)
USA300_TCH1516:
USA300HOU_1411 (gpsA)
VC40:
SAVC_06620 (gpsA)

⊟Genome Viewer

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments

• alignment of orthologues:
  CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
  
  
  COL             MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNII
  N315            MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNII
  NCTC8325        MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNII
  Newman          MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNII
  USA300_FPR3757  MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCHTNKKYLKYAKLDVNII
                  ************************************************************
  
  COL             ATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
  N315            ATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
  NCTC8325        ATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
  Newman          ATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
  USA300_FPR3757  ATSDMTKAIQFADIYLMALPTKAMREVASQINDKLTSKKTFIHVAKGIENGTFKRVSEMI
                  ****************************:*******************************
  
  COL             EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTND
  N315            EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTND
  NCTC8325        EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTND
  Newman          EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTND
  USA300_FPR3757  EDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKSVSKLTQDLFMNDYLRVYTND
                  ************************************************************
  
  COL             DLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGL
  N315            DLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGL
  NCTC8325        DLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGL
  Newman          DLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGL
  USA300_FPR3757  DLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTRGLAEISRLGEKLGADPMTFLGL
                  ************************************************************
  
  COL             GGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVD
  N315            GGIGDLIVTCISTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVD
  NCTC8325        GGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVD
  Newman          GGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVD
  USA300_FPR3757  GGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSEMNMVVEGIYTTKSVYHLAKEKNVD
                  ********** *************************************************
  
  COL             MPITNALYRVLFENISVKECVKDLMERDKKSE
  N315            MPITNALYRVLFENISVKECVKDLMERDKKSE
  NCTC8325        MPITNALYRVLFENISVKECVKDLMERDKKSE
  Newman          MPITNALYRVLFENISVKECVKDLMERDKKSE
  USA300_FPR3757  MPITNALYRVLFENISVKECVKDLMERDKKSE
                  ********************************