From AureoWiki
Jump to navigation Jump to search

NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1385 [new locus tag: NWMN_RS07800 ]
  • pan locus tag?: SAUPAN003937000
  • symbol: rpsA
  • pan gene symbol?: rpsA
  • synonym:
  • product: 30S ribosomal protein S1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1385 [new locus tag: NWMN_RS07800 ]
  • symbol: rpsA
  • product: 30S ribosomal protein S1
  • replicon: chromosome
  • strand: -
  • coordinates: 1551438..1552613
  • length: 1176
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGACTGAAGAATTCAATGAATCAATGATTAACGATATTAAAGAAGGTGACAAAGTCACT
    GGCGAGGTACAACAAGTTGAAGACAAGCAAGTTGTTGTTCATATCAACGGTGGTAAATTT
    AATGGGATTATTCCTATTAGTCAACTATCTACGCATCATATTGATAGCCCAAGTGAAGTT
    GTAAAAGAGGGCGACGAAGTTGAAGCATATGTCACTAAAGTTGAGTTTGATGAAGAAAAT
    GAAACTGGAGCTTACATCTTATCTAGAAGACAACTTGAAACTGAGAAGTCTTATAGTTAT
    TTACAAGAAAAATTAGATAATAATGAAATCATCGAAGCGAAAGTAACAGAAGTAGTTAAA
    GGTGGTTTGGTTGTTGATGTAGGACAAAGAGGTTTTGTTCCGGCTTCACTAATTTCAACA
    GACTTCATTGAGGATTTCTCTGTGTTTGATGGACAAACAATTCGTATTAAAGTTGAAGAA
    TTGGATCCTGAAAATAATAGAGTCATTTTAAGCCGTAAAGCAGTTGAACAAGAAGAAAAC
    GATGCTAAAAAAGATCAATTATTACAATCTTTAAATGAAGGCGATGTTATTGATGGTAAA
    GTAGCGCGTTTAACTCAATTTGGTGCATTTATAGACATTGGCGGTGTTGATGGTTTAGTG
    CATGTATCTGAACTTTCTCACGAACATGTTCAAACACCAGAAGAAGTAGTTTCAATTGGT
    CAAGATGTTAAAGTTAAAATTAAATCTATTGATAGAGATACAGAACGTATTTCATTATCA
    ATCAAAGATACGTTACCAACACCTTTCGAAAATATTAAAGGTCAATTCCACGAAAATGAT
    GTCATTGAAGGTGTCGTAGTAAGATTGGCAAACTTTGGTGCATTTGTTGAAATTGCACCA
    GGTGTACAAGGACTTGTACATATTTCTGAAATTGCACACAAACACATTGGTACGCCAGGT
    GAAGTGTTAGAACCTGGTCAACAAGTAAATGTTAAAATATTAGGTATTGATGAAGAGAAT
    GAAAGAGTATCACTATCTATTAAAGCAACATTACCAAACGAAGATGTTGTTGAAAGTGAT
    CCTTCTACGACTAAGGCGTACTTAGAAAACGAAGAAGAAGATAATCCAACAATTGGCGAT
    ATGATTGGTGATAAACTTAAAAATCTTAAACTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1385 [new locus tag: NWMN_RS07800 ]
  • symbol: RpsA
  • description: 30S ribosomal protein S1
  • length: 391
  • theoretical pI: 4.23557
  • theoretical MW: 43287
  • GRAVY: -0.390537

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein bS1 (TIGR00717; HMM-score: 286.5)
    and 8 more
    Genetic information processing Transcription Degradation of RNA polyribonucleotide nucleotidyltransferase (TIGR03591; EC 2.7.7.8; HMM-score: 147.8)
    guanosine pentaphosphate synthetase I/polyribonucleotide nucleotidyltransferase (TIGR02696; EC 2.7.-.-,2.7.7.8; HMM-score: 58.7)
    Genetic information processing Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase (TIGR00448; EC 2.7.7.6; HMM-score: 44.2)
    Genetic information processing Transcription Degradation of RNA ribonuclease R (TIGR02063; EC 3.1.-.-; HMM-score: 36.7)
    MAST domain (TIGR04204; HMM-score: 14.1)
    Metabolism Energy metabolism Glycolysis/gluconeogenesis carbon storage regulator (TIGR00202; HMM-score: 9.8)
    Signal transduction Regulatory functions RNA interactions carbon storage regulator (TIGR00202; HMM-score: 9.8)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, YajC subunit (TIGR00739; HMM-score: 8.5)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    OB (CL0021) S1; S1 RNA binding domain (PF00575; HMM-score: 252.3)
    and 8 more
    CusF_Ec; Copper binding periplasmic protein CusF (PF11604; HMM-score: 16.6)
    Cdc48_2-like (CL0402) CDC48_2; Cell division protein 48 (CDC48), domain 2 (PF02933; HMM-score: 15.9)
    OB (CL0021) S1_2; S1 domain (PF13509; HMM-score: 15.7)
    EXOSC1; Exosome component EXOSC1/CSL4 (PF10447; HMM-score: 12.4)
    RuvA_N; RuvA N terminal domain (PF01330; HMM-score: 10.5)
    no clan defined DUF1967; Domain of unknown function (DUF1967) (PF09269; HMM-score: 9.9)
    CsrA; Global regulator protein family (PF02599; HMM-score: 7.9)
    KOW (CL0107) KOW; KOW motif (PF00467; HMM-score: 7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.021822
    • TAT(Tat/SPI): 0.000535
    • LIPO(Sec/SPII): 0.000886
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGIIPISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQEKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIRIKVEELDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDGLVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQFHENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKILGIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=NWMN_1385&oldid=66888"