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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01666
  • pan locus tag?: SAUPAN004146000
  • symbol: SAOUHSC_01666
  • pan gene symbol?: glyS
  • synonym:
  • product: glycyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01666
  • symbol: SAOUHSC_01666
  • product: glycyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1579475..1580866
  • length: 1392
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAAAAGATATGGATACAATTGTTTCATTAGCAAAACACAGAGGTTTTGTGTTCCCT
    GGTAGTGATATTTACGGTGGTTTATCAAACACATGGGATTATGGTCCTTTAGGTGTTGAA
    TTAAAGAATAATGTTAAAAAAGCTTGGTGGCAAAAATTCATTACACAATCACCGTTTAAC
    GTTGGTATCGATGCTGCAATCTTAATGAATCCAAAAGTATGGGAAGCTTCAGGACACTTA
    AACAACTTCAACGACCCAATGATTGATAATAAAGATAGTAAAATTCGATATCGCGCTGAT
    AAATTAATTGAAGATTATATGCAAGATGTTAAAGGTGATGAAAACTTCATTGCCGATGGT
    TTAAGTTTTGAACAAATGAAAAAAATTATTGACGATGAAGGTATTGTTTGTCCTGTAAGT
    AAAACTGCTAACTGGACTGAAATTCGCCAATTCAATTTAATGTTTAAAACATTCCAAGGT
    GTAACTGAAGATTCTACAAATGAAATTTTCTTACGTCCTGAAACAGCACAAGGTATTTTT
    GTAAACTATAAAAACGTGCAACGTTCAATGCGTAAAAAATTACCATTTGGTATCGGTCAA
    ATTGGTAAATCATTCCGTAATGAAATCACTCCAGGTAACTTCATTTTCAGAACAAGAGAA
    TTTGAACAAATGGAACTTGAATTCTTCTGTAAACCTGGAGAAGAAATCGAATGGCAAAAT
    TATTGGAAAACTTTTGCAAGTGACTGGTTAACAAGCTTAAATATGAGCAGTGAAAATATG
    CGTTTACGTGATCATGATGAAGATGAATTATCTCATTACTCAAATGCAACAACTGATATT
    GAATATAAATTCCCATTTGGTTGGGGTGAGTTATGGGGTATCGCAAGTCGTACAGACTTC
    GACTTACGTAAACATGCTGAACACTCTGGTGAAGATTTCAGATACCATGATCCAGAAACG
    AACGAAAAATATATTCCATATTGTATCGAGCCATCACTTGGTGCAGATCGTGTAACATTA
    GCTTTCTTATGTGATGCATATGATGAAGAAGGCGTTGAAGGTAGTAAAGATGCACGTACA
    GTTTTACACTTCCATCCTGCATTAGCACCATATAAAGCAGCGATTTTACCTTTAAGTAAG
    AAATTATCTGGCGAAGCGATTAAGATTTTTGAGCAATTAAGTTCTAAATTCTCAATCGAT
    TTCGATGAATCACAATCTATCGGTAAAAGATACCGTCGTCAAGATGAAATCGGTACACCT
    TATTGTGTAACATTCGACTTTGATTCATTAGAAGATAATCAAGTTACAGTACGTGACAGA
    GATTCAATGGAACAAGTTCGTATGCCAATCTCAGAGTTAGAAGCTTTCTTAACTGAAAAA
    ACAAAATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1392

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01666
  • symbol: SAOUHSC_01666
  • description: glycyl-tRNA synthetase
  • length: 463
  • theoretical pI: 4.7417
  • theoretical MW: 53619.9
  • GRAVY: -0.588769

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.14?  ExPASy
    Glycine--tRNA ligase ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation glycine--tRNA ligase (TIGR00389; EC 6.1.1.14; HMM-score: 525.6)
    and 5 more
    Genetic information processing Protein synthesis tRNA aminoacylation threonine--tRNA ligase (TIGR00418; EC 6.1.1.3; HMM-score: 54.8)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00409; EC 6.1.1.15; HMM-score: 38.8)
    Genetic information processing Protein synthesis tRNA aminoacylation histidine--tRNA ligase (TIGR00442; EC 6.1.1.21; HMM-score: 37.7)
    Genetic information processing Protein synthesis tRNA aminoacylation proline--tRNA ligase (TIGR00408; EC 6.1.1.15; HMM-score: 28.5)
    Genetic information processing Protein synthesis tRNA aminoacylation serine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 15.9)
  • TheSEED  :
    • Glycyl-tRNA synthetase (EC 6.1.1.14)
    tRNA synthetase relationships  Glycyl-tRNA synthetase (EC 6.1.1.14)
  • PFAM:
    IIaaRS-ABD (CL0458) HGTP_anticodon; Anticodon binding domain (PF03129; HMM-score: 84.1)
    and 2 more
    tRNA_synt_II (CL0040) tRNA-synt_2b; tRNA synthetase class II core domain (G, H, P, S and T) (PF00587; HMM-score: 37.9)
    no clan defined PHF12_MRG_bd; PHD finger protein 12 MRG binding domain (PF16737; HMM-score: 12.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.012542
    • TAT(Tat/SPI): 0.000444
    • LIPO(Sec/SPII): 0.001066
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNNVKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKLIEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQGVTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRTREFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNATTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLGADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQLSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPISELEAFLTEKTKF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [4] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [4] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Gly) (transcription antitermination) regulon
    T-box(Gly)(5' cis-acting region)important in Amino acid metabolism; compare RegPrecise for N315 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  5. ↑ 5.0 5.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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