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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1246 [new locus tag: SAUSA300_RS06765 ]
  • pan locus tag?: SAUPAN003735000
  • symbol: acnA
  • pan gene symbol?: citB
  • synonym:
  • product: aconitate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1246 [new locus tag: SAUSA300_RS06765 ]
  • symbol: acnA
  • product: aconitate hydratase
  • replicon: chromosome
  • strand: +
  • coordinates: 1367131..1369836
  • length: 2706
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCAAATTTTAAAGAGCAATCAAAAAAACATTTTGACTTGAATGGCCAAAGTTAT
    ACTTACTATGATTTAAAAGCTGTAGAAGAGCAAGGTATTACTAAAGTTTCCAATTTACCT
    TATTCAATTCGTGTTTTGTTAGAATCTTTACTTCGTCAAGAAGATGATTTTGTAATTACA
    GACGATCATATTAAAGCTTTAAGTCAGTTTGGAAAAGATGGAAATGAAGGCGAGGTGCCA
    TTTAAACCTTCTCGTGTTATTTTACAAGATTTCACAGGTGTACCAGCCGTAGTTGATTTA
    GCTTCTTTACGTAAAGCAATGGATGACGTTGGGGGAGATATTACTAAAATTAATCCAGAA
    GTACCGGTGGATTTAGTTATTGACCACTCAGTTCAAGTGGATAGCTATGCAAATCCAGAA
    GCTCTTGAACGTAATATGAAATTAGAATTTGAACGTAACTATGAACGTTATCAGTTTTTA
    AATTGGGCAACGAAAGCATTTGATAATTACAATGCAGTTCCTCCTGCAACTGGAATAGTT
    CACCAAGTTAACTTAGAATATTTAGCAAGTGTTGTACATGTTCGTGATGTAGATGGTGAA
    AAAACTGCATTTCCAGATACATTAGTTGGTACTGATTCACATACAACAATGATAAATGGT
    ATTGGCGTACTAGGATGGGGTGTTGGTGGTATTGAAGCTGAAGCTGGAATGCTTGGACAA
    CCTTCTTATTTCCCAATTCCAGAGGTTATTGGTGTACGACTAGTAAATTCATTACCACAA
    GGCGCAACAGCAACTGATTTAGCGTTAAGAGTAACTCAAGAGCTACGTAAAAAAGGTGTT
    GTTGGTAAATTTGTGGAGTTCTTTGGTCCAGGTGTACAACATTTACCACTAGCAGACCGT
    GCTACAATTGCAAACATGGCACCAGAGTATGGAGCAACTTGCGGATTCTTCCCAGTTGAT
    GATGAATCTCTTAAATATATGAAGTTAACTGGTAGATCAGACGAACATATCGCGCTAGTA
    AAAGAATATTTGAAACAAAACCATATGTTCTTTGATGTTGAGAAAGAAGATCCTAATTAT
    ACAGATGTTATCGAATTGGATTTATCAACAGTTGAAGCATCGCTTTCAGGACCAAAACGT
    CCTCAAGATTTAATTTTCTTAAGTGATATGAAATCATCATTTGAAAATTCTGTAACAGCT
    CCAGCAGGCAACCAAGGACACGGTTTAGATAAAAGTGAATTTGATAAGAAAGCTGAAATT
    AACTTTAAAGATGGATCAAAAGCTACAATGAAAACAGGTGATATTGCAATAGCAGCAATT
    ACATCATGTACAAATACATCTAACCCTTATGTAATGTTAGGTGCAGGTTTAGTTGCTAAA
    AAAGCAGTTGAAAAAGGCTTGAAAGTTCCTGAATACGTTAAAACTTCTCTAGCACCAGGA
    TCAAAAGTTGTTACCGGATATTTAAGAGATGCTGGCTTACAACCTTATTTAGATGATTTA
    GGCTTCAACTTGGTTGGTTATGGATGTACAACTTGTATCGGTAATTCAGGTCCTTTATTA
    CCAGAAATTGAAAAAGCGATTGCTGATGAGGACCTATTAGTGACATCTGTATTATCTGGT
    AACCGTAACTTTGAAGGTCGTATCCATCCTCTTGTTAAAGCCAATTACCTAGCTTCACCA
    CAGTTAGTTGTTGCTTATGCATTAGCTGGAACGGTTGATATTGATTTACAAAATGAACCT
    ATTGGTAAAGGTAATGACGGTGAAGATGTATATTTGAAAGATATTTGGCCATCAATTAAA
    GAAGTTTCAGATACCGTTGATAGTGTTGTAACACCTGAATTATTTATTGAAGAATATAAT
    AACGTATACAATAACAACGAATTATGGAATGAGATTGATGTAACTGATCAACCTCTATAT
    GACTTTGATCCTAATTCAACATACATTCAAAATCCATCATTCTTCCAAGGATTATCTAAA
    GAACCGGGTACGATTGTTCCATTAAATGGTTTACGTGTTATGGGTAAATTCGGTGATTCT
    GTGACAACTGACCACATCTCTCCAGCAGGTGCAATTGGTAAAGATACGCCAGCTGGTAAA
    TATTTACAAGATCATCAAGTGCCTATTCGTGAATTTAATTCATATGGTTCAAGACGTGGT
    AATCACGAAGTAATGGTTCGAGGTACGTTTGCTAATATACGTATTAAAAACCAATTAGCG
    CCAGGTACTGAAGGTGGTTTTACAACTTATTGGCCAACAAATGAAGTAATGCCTATCTTT
    GATGCTGCAATGAAATATAAAGAAGATGGTACAGGTTTAGTTGTATTAGCTGGTAACGAT
    TATGGTATGGGTTCATCTCGTGACTGGGCAGCAAAAGGTACAAACTTATTAGGTGTTAAA
    ACAGTTATTGCACAAAGTTATGAACGTATCCATCGTTCAAATTTAGTTATGATGGGTGTA
    TTACCATTAGAGTTTAAAAAAGGTGAATCAGCTGATTCTCTTGGTCTAGATGGTACAGAA
    GAAATTTCTGTTAATATTGATGAAAATGTTCAACCACATGACTACGTCAAAGTTACTGCT
    AAGAAGCAAGATGGTGATTTGGTAGAATTTGACGCTATGGTTCGTTTTGACTCACTTGTT
    GAAATGGATTACTATCGTCACGGTGGAATTTTACAAATGGTTTTAAGAAATAAATTAGCG
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2706

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1246 [new locus tag: SAUSA300_RS06765 ]
  • symbol: AcnA
  • description: aconitate hydratase
  • length: 901
  • theoretical pI: 4.58662
  • theoretical MW: 98969
  • GRAVY: -0.276249

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.3?  ExPASy
    Aconitate hydratase Citrate = isocitrate
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle aconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 1440.3)
    and 11 more
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 785.6)
    Metabolism Energy metabolism TCA cycle aconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 240.6)
    Metabolism Energy metabolism TCA cycle putative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 239.1)
    homoaconitate hydratase family protein (TIGR01343; HMM-score: 118.3)
    3-isopropylmalate dehydratase, large subunit (TIGR02086; HMM-score: 113.8)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR02083; EC 4.2.1.33; HMM-score: 110.8)
    Metabolism Amino acid biosynthesis Aspartate family homoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 97.8)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, large subunit (TIGR00170; EC 4.2.1.33; HMM-score: 89.8)
    3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 48.5)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, small subunit (TIGR02084; EC 4.2.1.33; HMM-score: 37.5)
    Metabolism Amino acid biosynthesis Pyruvate family 3-isopropylmalate dehydratase, small subunit (TIGR00171; EC 4.2.1.33; HMM-score: 27.8)
  • TheSEED  :
    • Aconitate hydratase (EC 4.2.1.3)
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Aconitate hydratase (EC 4.2.1.3)
    and 2 more
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Aconitate hydratase (EC 4.2.1.3)
    Respiration Respiration - no subcategory Biogenesis of c-type cytochromes  Aconitate hydratase (EC 4.2.1.3)
  • PFAM:
    no clan defined Aconitase; Aconitase family (aconitate hydratase) (PF00330; HMM-score: 588.2)
    and 1 more
    Leu-IlvD (CL0364) Aconitase_C; Aconitase C-terminal domain (PF00694; HMM-score: 146.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: COFACTOR:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001879
    • TAT(Tat/SPI): 0.000137
    • LIPO(Sec/SPII): 0.000282
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIRVLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLASLRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFLNWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMINGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELRKKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSDEHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKSSFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPYVMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYGCTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAYALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYNNNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVTTDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLAPGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLGVKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYVKVTAKKQDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Fur* (repression) regulon, CcpA regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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