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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0844 [new locus tag: SAUSA300_RS04560 ]
  • pan locus tag?: SAUPAN003040000
  • symbol: SAUSA300_0844
  • pan gene symbol?: ndh2a
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0844 [new locus tag: SAUSA300_RS04560 ]
  • symbol: SAUSA300_0844
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 921937..923145
  • length: 1209
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGGCTCAAGATCGTAAAAAAGTACTTGTACTTGGTGCTGGTTATGCAGGTTTACAAACT
    GTAACTAAATTGCAAAAAGCGATATCAACAGAAGAAGCAGAAATTACGCTTATTAATAAA
    AATGAATATCACTATGAAGCAACATGGTTACATGAAGCATCAGCAGGTACACTAAACTAT
    GAAGATGTATTATATCCTGTGGAAAGTGTCTTGAAGAAAGACAAAGTGAACTTTGTTCAA
    GCAGAAGTAACAAAAATTGACCGTGATGCTAAAAAGGTAGAAACAAATCAAGGTATTTAT
    GACTTTGATATTTTAGTAGTAGCATTAGGTTTCGTTAGTGAAACATTCGGCATCGAAGGT
    ATGAAAGATCATGCTTTCCAAATTGAAAATGTTATCACAGCACGTGAATTATCACGTCAT
    ATCGAAGACAAATTTGCTAACTATGCAGCATCAAAAGAAAAAGATGATAACGATTTATCT
    ATCTTAGTTGGTGGTGCTGGATTCACTGGTGTTGAATTCTTAGGTGAATTAACAGACAGA
    ATTCCTGAATTATGTAGCAAATATGGTGTGGATCAAAATAAAGTTAAAATCACTTGTGTT
    GAAGCAGCACCTAAAATGTTACCAATGTTCTCAGAAGAATTAGTTAACCACGCAGTTAGC
    TACTTAGAAGACCGCGGTGTTGAATTTAAAATTGCTACACCAATCGTTGCTTGTAACGAA
    AAAGGTTTTGTAGTTGAAGTAGATGGTGAAAAACAACAATTAAATGCAGGTACTTCAGTA
    TGGGCAGCTGGTGTACGTGGTAGTAAATTAATGGAAGAATCATTTGAAGGCGTTAAACGT
    GGACGTATCGTTACAAAGCAAGATTTAACAATCAATGGTTACGACAACATTTTTGTTATT
    GGTGACTGTTCAGCGTTTATCCCAGCTGGAGAAGAACGTCCATTACCAACTACAGCACAA
    ATTGCAATGCAACAAGGTGAAAGTGTTGCTAAAAACATTAAACGCATCTTAAACGGTGAA
    TCAACTGAAGAATTCGAATACGTTGATCGTGGAACTGTTTGTTCTTTAGGTTCACATGAC
    GGTGTAGGTATGGTATTTGGTAAACCTATCGCTGGTAAAAAAGCAGCATTCATGAAAAAA
    GTGATTGATACACGTGCGGTATTCAAAATCGGTGGTATCGGTTTAGCATTCAAAAAAGGT
    AAATTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1209

⊟Protein[edit | edit source]

Protein Data Bank: 5NA1
Protein Data Bank: 5NA4

⊟General[edit | edit source]

  • locus tag: SAUSA300_0844 [new locus tag: SAUSA300_RS04560 ]
  • symbol: SAUSA300_0844
  • description: hypothetical protein
  • length: 402
  • theoretical pI: 5.14656
  • theoretical MW: 44104.1
  • GRAVY: -0.176119

⊟Function[edit | edit source]

  • reaction:
    EC 1.6.99.-?  ExPASy
  • TIGRFAM:
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 96.8)
    and 7 more
    oxidoreductase/SelD-related fusion protein (TIGR04369; HMM-score: 61.7)
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 53)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 33.4)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 33.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine glycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 17.4)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 16.6)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 9.6)
  • TheSEED  :
    • NADH dehydrogenase (EC 1.6.99.3)
    Respiration Electron donating reactions Respiratory dehydrogenases 1  NADH dehydrogenase (EC 1.6.99.3)
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 145.9)
    and 9 more
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 37.5)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 29.6)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 24.4)
    Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 15.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 13.5)
    AlaDh_PNT_C; Alanine dehydrogenase/PNT, C-terminal domain (PF01262; HMM-score: 12.7)
    Maf (CL0269) Ham1p_like; Ham1 family (PF01725; HMM-score: 12.7)
    NADP_Rossmann (CL0063) K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 12.6)
    no clan defined Tau95; RNA polymerase III transcription factor (TF)IIIC subunit (PF09734; HMM-score: 11.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.159156
    • TAT(Tat/SPI): 0.029488
    • LIPO(Sec/SPII): 0.008793
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYHYEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDFDILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLSILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHAVSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFEGVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIKRILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGGIGLAFKKGKF

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_1080(ftsZ)cell division protein FtsZ  [1] (data from MRSA252)
    SAUSA300_1362(hup)DNA-binding protein HU  [1] (data from MRSA252)
    SAUSA300_1640(icd)isocitrate dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0996(lpdA)dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAUSA300_0993(pdhA)pyruvate dehydrogenase E1 component, alpha subunit  [1] (data from MRSA252)
    SAUSA300_0994(pdhB)pyruvate dehydrogenase E1 component, beta subunit  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2204(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SAUSA300_0524(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SAUSA300_0525(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SAUSA300_2185(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SAUSA300_1134(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    SAUSA300_1603(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SAUSA300_2199(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SAUSA300_2202(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SAUSA300_1306(sucA)2-oxoglutarate dehydrogenase E1 component  [1] (data from MRSA252)
    SAUSA300_1305(sucB)dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SAUSA300_0533(tuf)elongation factor Tu  [1] (data from MRSA252)
    SAUSA300_0995branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    SAUSA300_1656universal stress protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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