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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2104 [new locus tag: SAUSA300_RS11600 ]
  • pan locus tag?: SAUPAN005492000
  • symbol: glmS
  • pan gene symbol?: glmS
  • synonym:
  • product: glucosamine--fructose-6-phosphate aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2104 [new locus tag: SAUSA300_RS11600 ]
  • symbol: glmS
  • product: glucosamine--fructose-6-phosphate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2270195..2272000
  • length: 1806
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGTGTGGAATTGTTGGTTATATTGGCTATGATAATGCCAAAGAATTATTATTAAAAGGT
    TTAGAAAAATTAGAATACAGAGGTTATGACTCTGCAGGTATCGCAGTAGTAAATGATGAT
    AATACAACTGTATTTAAAGAAAAAGGTCGTATTGCAGAATTACGTAAAGTTGCTGATAGT
    AGCGATTTTGATGGACCTGTTGGAATCGGTCACACACGTTGGGCAACACACGGTGTACCG
    AATCATGAAAACTCTCATCCACATCAATCATCAAATGGCCGTTTTACTCTAGTTCATAAC
    GGTGTTATTGAAAACTATGAAGAGTTAAAAGGTGAATACTTACAAGGTGTATCATTCATT
    TCAGAAACAGATACAGAAGTTATCGTTCAATTAGTTGAATACTTTTCAAATCAAGGACTT
    TCAACTGAAGAAGCATTTACAAAAGTTGTGTCATTATTACATGGTTCATATGCATTAGGT
    TTATTAGATGCTGAAGACAAAGACACAATCTATGTTGCTAAAAATAAATCACCATTATTA
    TTAGGTGTTGGTGAAGGTTTCAATGTTATCGCATCAGACGCACTTGCAATGTTACAAGTG
    ACAAGCGAATATAAAGAAATCCATGACCATGAAATCGTTATTGTTAAAAAAGATGAAGTT
    ATTATTAAAGATGCAGATGGAAACGTTGTAGAACGTGATTCATATATTGCTGAAATTGAT
    GCATCAGATGCTGAAAAAGGTGTTTATGCACACTACATGTTAAAAGAAATTCATGAACAA
    CCAGCAGTAATGCGTCGTATTATTCAAGAATATCAAGATGCAGAAGGTAACTTGAAAATT
    GATCAAGACATCATCAATGATGTTAAAGAAGCAGACCGCATTTACGTTATTGCAGCAGGT
    ACAAGCTACCATGCAGGTTTAGTAGGTAAAGAATTTTTAGAAAAATGGGCTGGCGTACCA
    ACTGAAGTACACGTTGCATCAGAGTTTGTCTACAACATGCCATTATTATCTGAAAAACCA
    TTGTTCGTTTATATTTCTCAATCAGGTGAAACTGCAGATAGCCGCGCCGTATTAGTTGAA
    ACTAATAAATTAGGTCATAAATCATTAACAATCACTAATGTTGCAGGTTCAACTTTATCA
    CGTGAAGCAGACCACACATTGTTATTACACGCGGGTCCTGAAATCGCAGTTGCATCTACA
    AAAGCATATACTGCACAAATTGCAGTATTATCAATCTTGTCTCAAATCGTTGCAAAAGAG
    CATGGTCGTGAAGCAGATATTGATTTATTGAGAGAATTAGCAAAAGTAACAACAGCAATA
    GAAGCAATTGTTGACGATGCACCAATTATGGAACAAATTGCTACAGATTTCTTAGAAACA
    ACACGCAATGCATTCTTTATCGGACGTACTATTGACTATAACGTAAGTTTAGAAGGTGCG
    TTAAAACTTAAAGAAATTTCTTACATTCAAGCAGAAGGTTTTGCTGGTGGAGAACTTAAA
    CATGGTACAATTGCCTTAATCGAAGAAGGTACACCAGTTGTAGGTTTAGCAACACAAGAG
    AAAGTTAATTTATCAATTCGTGGTAACGTTAAAGAGGTAGTAGCACGTGGTGCACATCCA
    TGTATTATTTCTATGGAGGGTCTTGAAAAAGAAGGCGACACTTATGTCATTCCTCATGTA
    CATGAATTGTTAACGCCATTAGTATCAGTGGTTGCATTACAATTAATTTCATACTATGCA
    GCATTACACAGAGATTTAGATGTTGATAAACCACGTAACCTTGCTAAATCAGTTACTGTG
    GAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1806

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2104 [new locus tag: SAUSA300_RS11600 ]
  • symbol: GlmS
  • description: glucosamine--fructose-6-phosphate aminotransferase
  • length: 601
  • theoretical pI: 4.67212
  • theoretical MW: 65849
  • GRAVY: -0.0895175

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.16?  ExPASy
    Glutamine--fructose-6-phosphate transaminase (isomerizing) L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 749.4)
    Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 749.4)
    and 9 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 111.3)
    Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 50.4)
    6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 39.4)
    asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.3)
    exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 31.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramic acid 6-phosphate etherase (TIGR00274; EC 4.2.1.126; HMM-score: 30.1)
    putative sugar isomerase, AgaS family (TIGR02815; HMM-score: 24.4)
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 22.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis protein EgtC (TIGR03442; HMM-score: 12.8)
  • TheSEED  :
    • Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)
    Carbohydrates One-carbon Metabolism Formaldehyde assimilation: Ribulose monophosphate pathway  Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)
    and 2 more
    Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16)
  • PFAM:
    SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 169.8)
    and 3 more
    NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 79.5)
    GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 56.2)
    GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 23.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004969
    • TAT(Tat/SPI): 0.000441
    • LIPO(Sec/SPII): 0.000872
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTVFKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGVIENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALGLLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKDEVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEGNLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMPLLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAGPEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIMEQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIEEGTPVVGLATQEKVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTPLVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_0532(fusA)elongation factor G  [1] (data from MRSA252)
    SAUSA300_0525(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: glmS leader (RNA cleavage and degradation) regulon
    glmS leader(RNA)important in Amino sugar metabolism; regulatory site identified based on RegPrecise data for N315 RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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