PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_002115
• pan locus tag^?: SAUPAN005492000
• symbol: glmS
• pan gene symbol^?: glmS
• synonym:
• product: glutamine--fructose-6-phosphate transaminase (isomerizing)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_002115
• symbol: glmS
• product: glutamine--fructose-6-phosphate transaminase (isomerizing)
• replicon: chromosome
• strand: -
• coordinates: 2124873..2126678
• length: 1806
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  
  ATGTGTGGAATTGTTGGTTATATTGGCTATGATAATGCCAAAGAATTATTATTAAAAGGT
  TTAGAAAAATTAGAATACAGAGGTTATGACTCTGCAGGTATCGCAGTAGTAAATGATGAT
  AATACAACTGTATTTAAAGAAAAAGGTCGTATTGCAGAATTACGTAAAGTTGCTGATAGT
  AGCGATTTTGATGGACCTGTTGGAATCGGTCACACACGTTGGGCAACACACGGTGTACCG
  AATCATGAAAACTCTCATCCACATCAATCATCAAATGGCCGTTTTACTCTAGTTCATAAC
  GGTGTTATTGAAAACTATGAAGAGTTAAAAGGTGAATACTTACAAGGTGTATCATTCATT
  TCAGAAACAGATACAGAAGTAATCGTTCAATTAGTTGAATACTTTTCAAATCAAGGACTT
  TCAACTGAAGAAGCATTTACAAAAGTTGTGTCATTATTACATGGTTCATATGCATTAGGT
  TTATTAGATGCTGAAGACAAAGACACAATCTATGTTGCTAAAAATAAATCACCATTATTA
  TTAGGTGTTGGTGAAGGTTTCAATGTTATCGCATCAGACGCACTTGCAATGTTACAAGTG
  ACAAGCGAATATAAAGAAATCCATGACCATGAAATCGTTATTGTTAAAAAAGATGAAGTT
  ATTATTAAAGATGCAGATGGAAACGTTGTAGAACGTGATTCATATATTGCTGAAATTGAT
  GCATCAGATGCTGAAAAAGGTGTTTATGCACACTACATGTTAAAAGAAATTCATGAACAA
  CCAGCAGTAATGCGTCGTATTATTCAAGAATATCAAGATGCAGAAGGTAACTTGAAAATT
  GATCAAGACATCATCAATGATGTTAAAGAAGCAGACCGCATTTACGTTATTGCAGCAGGT
  ACAAGCTACCATGCAGGTTTAGTAGGTAAAGAATTTTTAGAAAAATGGGCTGGCGTACCA
  ACTGAAGTACACGTTGCATCAGAGTTTGTCTACAACATGCCATTATTATCTGAAAAACCA
  TTGTTCGTTTATATTTCTCAATCAGGTGAAACTGCAGATAGCCGCGCCGTATTAGTTGAA
  ACTAATAAATTAGGTCATAAATCATTAACAATCACTAATGTTGCAGGTTCAACTTTATCA
  CGTGAAGCAGACCACACATTGTTATTACACGCGGGTCCTGAAATCGCAGTTGCATCTACA
  AAAGCATATACTGCACAAATTGCAGTATTATCAATCTTGTCTCAAATCGTTGCAAAAGAG
  CACGGTCGTGAAGCAGATATTGATTTATTGAGAGAATTAGCAAAAGTAACAACAGCAATA
  GAAGCAATTGTTGACGATGCACCAATTATGGAACAAATTGCTACAGATTTCTTAGAAACA
  ACACGCAATGCATTCTTTATCGGACGTACTATTGACTATAACGTAAGTTTAGAAGGTGCG
  TTAAAACTTAAAGAAATTTCTTACATTCAAGCAGAAGGTTTTGCTGGTGGAGAACTTAAA
  CATGGTACGATTGCATTAATCGAAGATGGCACACCAGTTGTAGCTTTAGCGACTCAAGAA
  AACGTTAATTTATCAATTCGTGGTAATGTTAAAGAGGTAGTAGCACGTGGTGCGCATCCA
  TGTATCATTTCTATGGAGGGTCTTGAAAAAGAAGGCGACACTTATGTCATTCCTCATGTA
  CATGAATTGTTAACGCCATTAGTATCAGTTGTTGCATTACAATTAATTTCATACTATGCA
  GCATTACACAGAGATTTAGATGTTGATAAACCACGTAACCTTGCTAAATCAGTTACTGTG
  GAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1806
  

⊟Protein

⊟General

• locus tag: JSNZ_002115
• symbol: GlmS
• description: glutamine--fructose-6-phosphate transaminase (isomerizing)
• length: 601
• theoretical pI: 4.64043
• theoretical MW: 65834.9
• GRAVY: -0.0851913

⊟Function

• reaction:
  EC 2.6.1.16?  ExPASy
  Glutamine--fructose-6-phosphate transaminase (isomerizing) L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 750.7)
  Metabolism Central intermediary metabolism Amino sugars glutamine-fructose-6-phosphate transaminase (isomerizing) (TIGR01135; EC 2.6.1.16; HMM-score: 750.7)
  and 9 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis amidophosphoribosyltransferase (TIGR01134; EC 2.4.2.14; HMM-score: 111.3)
  Metabolism Amino acid biosynthesis Aspartate family asparagine synthase (glutamine-hydrolyzing) (TIGR01536; EC 6.3.5.4; HMM-score: 50.4)
  6-phospho 3-hexuloisomerase (TIGR03127; EC 5.3.-.-; HMM-score: 39.5)
  asparagine synthase family amidotransferase (TIGR03104; HMM-score: 33.3)
  exosortase A system-associated amidotransferase 1 (TIGR03108; HMM-score: 31.8)
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramic acid 6-phosphate etherase (TIGR00274; EC 4.2.1.126; HMM-score: 30.1)
  putative sugar isomerase, AgaS family (TIGR02815; HMM-score: 25.9)
  Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 23.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs ergothioneine biosynthesis protein EgtC (TIGR03442; HMM-score: 13)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  SIS (CL0067) SIS; SIS domain (PF01380; HMM-score: 175.3)
  and 3 more
  NTN (CL0052) GATase_6; Glutamine amidotransferase domain (PF13522; HMM-score: 77.6)
  GATase_7; Glutamine amidotransferase domain (PF13537; HMM-score: 55.3)
  GATase_4; Glutamine amidotransferases class-II (PF13230; HMM-score: 22.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9692
  • Cytoplasmic Membrane Score: 0.0052
  • Cell wall & surface Score: 0.0011
  • Extracellular Score: 0.0245
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004969
  • TAT(Tat/SPI): 0.000441
  • LIPO(Sec/SPII): 0.000872
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTVFKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGVIENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALGLLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKDEVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEGNLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMPLLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAGPEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIMEQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIEDGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTPLVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for COL, N315, Newman, USA300_FPR3757

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications