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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1358 [new locus tag: SAUSA300_RS07405 ]
  • pan locus tag?: SAUPAN003929000
  • symbol: ndk
  • pan gene symbol?: ndk
  • synonym:
  • product: nucleoside diphosphate kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_1358 [new locus tag: SAUSA300_RS07405 ]
  • symbol: ndk
  • product: nucleoside diphosphate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1525749..1526180
  • length: 432
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGATTAAACCAGATGCAGTACAAAGAAATCTAATTGGTGAAGTAATTTCAAGAATTGAA
    AGAAAAGGACTAAAACTTGTCGGTGGTAAATTAATGCAAGTACCAATGGAACTTGCTGAA
    ACACATTATGGTGAACACCAAGGTAAACCATTTTATAATGATTTAATTTCATTTATTACA
    TCAGCACCAGTGTTCGCAATGGTAGTTGAAGGTGAAGATGCAGTTAATGTATCTAGACAT
    ATTATTGGCAGCACCAATCCTTCAGAAGCTTCACCAGGATCAATTAGAGGTGATTTAGGT
    TTAACTGTTGGTAGAAATATCATTCACGGTTCAGATTCATTAGAGTCTGCTGAACGTGAA
    ATTAATCTATGGTTTAATGAAAATGAAATTACTAGCTATGCTTCACCACGTGATGCATGG
    TTATATGAATAA
    60
    120
    180
    240
    300
    360
    420
    432

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_1358 [new locus tag: SAUSA300_RS07405 ]
  • symbol: Ndk
  • description: nucleoside diphosphate kinase
  • length: 143
  • theoretical pI: 4.79684
  • theoretical MW: 15796.7
  • GRAVY: -0.257343

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.4.6?  ExPASy
    Nucleoside-diphosphate kinase ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
  • TIGRFAM:
  • TheSEED  :
    • Nucleoside diphosphate kinase (EC 2.7.4.6)
    Nucleosides and Nucleotides Purines Purine conversions  Nucleoside diphosphate kinase (EC 2.7.4.6)
    and 1 more
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Nucleoside diphosphate kinase (EC 2.7.4.6)
  • PFAM:
    no clan defined NDK; Nucleoside diphosphate kinase (PF00334; HMM-score: 183.1)
    and 1 more
    TIM_barrel (CL0036) OMPdecase; Orotidine 5'-phosphate decarboxylase / HUMPS family (PF00215; HMM-score: 11.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006052
    • TAT(Tat/SPI): 0.004164
    • LIPO(Sec/SPII): 0.000708
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMELAETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIRGDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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