PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2183 [new locus tag: SAUSA300_RS12045 ]
• pan locus tag^?: SAUPAN005681000
• symbol: adk
• pan gene symbol^?: adk
• synonym:
• product: adenylate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2183 [new locus tag: SAUSA300_RS12045 ]
• symbol: adk
• product: adenylate kinase
• replicon: chromosome
• strand: -
• coordinates: 2360199..2360846
• length: 648
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914772 NCBI
• RefSeq: YP_494818 NCBI
• BioCyc: see SAUSA300_RS12045
• MicrobesOnline: 1293698 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAATATCATTTTGATGGGTTTACCTGGCGCAGGTAAAGGAACTCAAGCAAGTGAAATT
  GTCAAGAAATTCCCAATACCCCACATTTCAACTGGTGACATGTTCAGAAAAGCTATAAAA
  GAAGAAACTGAATTAGGTAAAGAAGCTAAGTCTTATATGGACCGTGGCGAATTAGTTCCT
  GATGAAGTGACTGTAGGTATCGTTAAGGAAAGAATTTCTGAAGACGATGCAAAAAAAGGC
  TTTTTATTAGATGGCTTCCCAAGAACAATCGAGCAAGCTGAGGCATTAAATAATATTATG
  TCTGAGCTTGACAGAAACATTGATGCTGTCATCAATATCGAAGTTCCGGAAGAAGAATTA
  ATGAACCGTCTTACAGGTCGTCGAATCTGTGAGTCATGTGGTACAACGTATCATCTTGTA
  TTTAATCCTCCGAAGGTCGAAGGTATTTGTGATATCGATGGTGGTAAATTGTATCAACGA
  GAAGATGATAATCCTGAAACGGTAGCTAATCGTTTGAGTGTTAATATTAAACAATCTAAG
  CCTATTTTAGATTTCTATGATCAAAAAGGTGTATTGAAAAATATTGATGGTTCAAAAGAT
  ATTAGCGACGTTACCAAAGATGTCATTGATATTTTAGATCATTTGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2183 [new locus tag: SAUSA300_RS12045 ]
• symbol: Adk
• description: adenylate kinase
• length: 215
• theoretical pI: 4.5477
• theoretical MW: 23974.2
• GRAVY: -0.416279

⊟Function

• reaction:
  EC 2.7.4.3?  ExPASy
  Adenylate kinase ATP + AMP = 2 ADP
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions adenylate kinase (TIGR01351; EC 2.7.4.-; HMM-score: 271)
  and 5 more
  UMP-CMP kinase family (TIGR01359; EC 2.7.4.-; HMM-score: 140.9)
  adenylate kinase (TIGR01360; EC 2.7.4.3; HMM-score: 126.1)
  putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 22.5)
  carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 12.7)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions dTMP kinase (TIGR00041; EC 2.7.4.9; HMM-score: 11.8)
• TheSEED  :
  • Adenylate kinase (EC 2.7.4.3)
  Nucleosides and Nucleotides Purines Purine conversions  Adenylate kinase (EC 2.7.4.3)
• PFAM:
  P-loop_NTPase (CL0023) ADK; Adenylate kinase (PF00406; HMM-score: 203.5)
  and 9 more
  AAA_17; AAA domain (PF13207; HMM-score: 112)
  no clan defined ADK_lid; Adenylate kinase, active site lid (PF05191; HMM-score: 65.5)
  P-loop_NTPase (CL0023) AAA_18; AAA domain (PF13238; HMM-score: 30.2)
  AAA_33; AAA domain (PF13671; HMM-score: 28.9)
  Hydin_ADK; Hydin Adenylate kinase-like domain (PF17213; HMM-score: 19.2)
  Thymidylate_kin; Thymidylate kinase (PF02223; HMM-score: 15.4)
  KTI12; Chromatin associated protein KTI12 (PF08433; HMM-score: 14.2)
  no clan defined DUF5024; Domain of unknown function (DUF5024) (PF16427; HMM-score: 13.7)
  Zn_Beta_Ribbon (CL0167) zf-RRN7; Zinc-finger of RNA-polymerase I-specific TFIIB, Rrn7 (PF11781; HMM-score: 9.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029489
  • TAT(Tat/SPI): 0.002665
  • LIPO(Sec/SPII): 0.003656
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161373 NCBI
• RefSeq: YP_494818 NCBI
• UniProt: A0A0H2XH09 UniProt

⊟Protein sequence

• MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETELGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSELDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQREDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: adk < secY < rplO < rpmD < rpsE < rplR < rplF < rpsH < rpsN < rplE < rplX < rplN < rpsQ < rpmC < rplP < rpsC < rplV < rpsS < rplB < rplW < rplD < rplC < rpsJ

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications