PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1641 [new locus tag: SAUSA300_RS08955 ]
• pan locus tag^?: SAUPAN004320000
• symbol: gltA
• pan gene symbol^?: citZ
• synonym:
• product: citrate synthase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1641 [new locus tag: SAUSA300_RS08955 ]
• symbol: gltA
• product: citrate synthase
• replicon: chromosome
• strand: -
• coordinates: 1799608..1800729
• length: 1122
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914350 NCBI
• RefSeq: YP_494336 NCBI
• BioCyc: see SAUSA300_RS08955
• MicrobesOnline: 1293156 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGGCAGAATTACAAAGAGGTTTAGAAGGGGTTATCGCAGCGGAGACTAAAATAAGTTCA
  ATTATTGAAAGTCAATTGACTTATGCCGGCTATGATATTGATGATCTAGCTGAAAATGCG
  CAATTTGAAGAAGTTATTTTCCTATTATGGAACTATAGATTGCCAAACGAAGAAGAGCTT
  GCTCATCTAAAAGGGAAATTAAATCAATACATGACATTAAATCCTCGTGTGTATACACAT
  TTTGAGGAGTATGTTACAGATCACGTGCATCCAATGACAGCATTACGTACGTCATTATCA
  TATATTGCACATTTCGATCCTGATGCTGAAAATGAATCAGATGAAAATCGTTATGAAAGA
  GCAATGCGTATACAGGCTAAAGTAGCATCATTAGTTACAGCGTTTGCTCGAGTAAGACAA
  GATAAAGAACCACTTAAGCCTAATCCTGACTTAAGTTATGCGGCAAACTTCCTATATATG
  TTACGTGGGGAATTACCAACAGATATAGAAGTAGAAGCCTTCAATAAAGCACTTATTTTA
  CACGCTGATCATGAGTTGAACGCATCTGCATTTACGGCACGTTGTGCGGTATCATCATTG
  TCAGATATGTACTCAGGTATTGTAGCAGCCGTAGGTTCTCTGAAAGGGCCATTACATGGT
  GGTGCAAACGAACAAGTTATGACGATGTTATCTGAGATTGGGTCAATTGAAAATGTTGAT
  GCTTACTTAGATGAAAAATTTGCTAATAAAGATAAAGTAATGGGCTTCGGTCATCGTGTA
  TATAAAGATGGTGATCCTAGAGCGAAATATTTAAGAGAAATGAGCCGTCAAATTACGAAA
  GACGCTGGTCGTGAAGAATTATTTGAAATGTCAGTGAAAATGGAAAAACGTATGGCAGAA
  GAAAAAGGATTAATTCCTAATGTTGATTTTTATAGTGCGAGTGTTTATCACTGTATGGAA
  ATACCTCATGACTTATTCACGCCAATCTTTGCTGTAAGTCGTTCTGCAGGATGGATTGCT
  CATATTTTAGAACAATATAAAGATAATAGAATTATGCGTCCTAGAGCGAAATATATTGGC
  GAAACGAATCGTAAGTATATCCCGCTTGAAGAAAGAAAATAA
  60
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  1122
  

⊟Protein

⊟General

• locus tag: SAUSA300_1641 [new locus tag: SAUSA300_RS08955 ]
• symbol: GltA
• description: citrate synthase
• length: 373
• theoretical pI: 5.33842
• theoretical MW: 42593.1
• GRAVY: -0.416086

⊟Function

• reaction:
  EC 2.3.3.5?  ExPASy
  2-methylcitrate synthase Propanoyl-CoA + H₂O + oxaloacetate = (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
• TIGRFAM:
  2-methylcitrate synthase/citrate synthase II (TIGR01800; HMM-score: 490.6)
  and 2 more
  Metabolism Energy metabolism TCA cycle citrate (Si)-synthase (TIGR01798; EC 2.3.3.1; HMM-score: 263.9)
  citrate (Si)-synthase, eukaryotic (TIGR01793; EC 2.3.3.1; HMM-score: 83.6)
• TheSEED  :
  • Citrate synthase (si) (EC 2.3.3.1)
  Carbohydrates Central carbohydrate metabolism TCA Cycle  Citrate synthase (si) (EC 2.3.3.1)
  and 2 more
  Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Citrate synthase (si) (EC 2.3.3.1)
  Respiration Respiration - no subcategory Biogenesis of c-type cytochromes  Citrate synthase (si) (EC 2.3.3.1)
• PFAM:
  no clan defined Citrate_synt; Citrate synthase, C-terminal domain (PF00285; HMM-score: 425.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002539
  • TAT(Tat/SPI): 0.000292
  • LIPO(Sec/SPII): 0.000332
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161124 NCBI
• RefSeq: YP_494336 NCBI
• UniProt: A0A0H2XGL1 UniProt

⊟Protein sequence

• MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEEVIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: icd < gltA

⊟Regulation

• regulator: CcpA regulon

  CcpA

  (TF)

  important in Carbon catabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications