PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0902 [new locus tag: SAUSA300_RS04855 ]
• pan locus tag^?: SAUPAN003171000
• symbol: pepF
• pan gene symbol^?: pepF
• synonym:
• product: oligoendopeptidase F

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0902 [new locus tag: SAUSA300_RS04855 ]
• symbol: pepF
• product: oligoendopeptidase F
• replicon: chromosome
• strand: +
• coordinates: 989609..991417
• length: 1809
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914117 NCBI
• RefSeq: YP_493602 NCBI
• BioCyc: see SAUSA300_RS04855
• MicrobesOnline: 1292417 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGAGTCAACAATTATCGAGAGAAGAACAGGAACGTAAATATCCTGAATATACATGGGAC
  TTAACAACAATTTTCAAAGATGATGAAGCTTTTGAGGCTGCATTTAAAGAAGTTGAAAAT
  GAGTTAGGCAAAGAAGAACAATTTAAAGGACACATTGGTGATAGTGCTGAGACATTATAC
  AATGCGTTAGAATTAGAAGATACATTAGGTACTAAATTAGAAAAAGTATATGTATACGCG
  CACCTAAAACAAGACCAAGATACAACGAACGACAAGTATACTGGTATGGAGTCAAGAGCA
  CATCAATTAATTATTAAATTTAGCTCGGCATGGAGTTTCTTAGTGCCAGAGATTTTACAA
  ATTGATGAAGATAAAATTCAATCATTTGTAAATTCATATGATAAATTACAAAAATTCGCA
  TTTGATTTGAAGTTGATTAATGAAAAACGTCCTCATATTTTAGATGCTGAAACTGAAAAG
  TTATTAACAGAAGCGCAGGACGCGTTATCAACGCCATCAAATGTATACGGTATGTTTAGC
  AACGCTGATTTAGTATTTGAAGATGCGATAGATAAAGATGGAAATGCACACCCGTTAACA
  CAAGGTACATTTATTAAGTATTTAGAAACAGATGATCGCAAACTAAGAGAAAGTGCTTTT
  AGAAATGTATATAAAGCATATGGTGCTCATAATAATACGCTTGGCGCTACGCTAGCAGGT
  GAAGTGAAGAAAAATGTATTTAATGCTCGTACACACAATTACAAAACTGCAAGAGAAAAA
  GCATTGAGTAATAATCATATTCCAGAAAATGTATATGACAATCTAGTAAAAACTGTACAT
  AAATATTTACCATTGCTACATAGATATACTGAATTGCGCAAAGAATTGCTAGGTTTAGAT
  GACTTGAAAATGTATGATTTATATACACCATTAATTAAAGATATTAAGTTTGAAATGCCT
  TATGAAGAAGCTAAAGAGTGGATGTTAAAAGCATTAGAACCAATGGGTGAAGAATATTTA
  AATGTAGTTAAAGAAGGCTTAAACAATCGTTGGGTCGATGTCTATGAGAATAAAGGTAAA
  CGTTCAGGTGGCTATTCATCAGGTGCACATTTAACTAATCCATTTATTCTACTTAACTGG
  TCTAATACTATTTCAGACTTATACACATTAGTTCATGAATTTGGGCATTCAGCACATAGT
  TACTTCAGTAGAAAATTCCAACCGTCAAATTCTAGTGACTACACTATTTTTGTCGCTGAA
  GTTGCATCAACTTGTAACGAAGCACTTTTAAGTGATTATATGGATAAACATCTTGATGAT
  GAAAAACGCTTATTATTATTAAACCAAGAATTAGAACGTTTCAGAGCTACATTATTCCGA
  CAAACAATGTTCGCAGAATTTGAGCATAAAATTCATGCAATTGAAGAAGCAGGTGAACCA
  TTAACGCCAACTAGAATGAATGAAGAATATGCCAAATTAAATAAATTATACTTCGGTGAT
  TCTGTAGAAACTGATGAAGATATTAGTAAGGAATGGTCACGTATTCCACACTTCTATATG
  AATTATTATGTATATCAATACGCAACTGGTTACAGTGCAGCTCAAAGCTTAAGTCATCAA
  ATTTTAACAGAAGGTAAGCCAGCAGTAGATAGATATATTAATGAATTCTTGAAAAAAGGT
  AGCTCAAATTATCCAATTGAGATATTAAAAAATGCTGGTGTAGATATGACAACACCTGAA
  CCAATTGAACAAGCTTGTGAAGTTTTTGAACAAAAATTGAACGCTTTTGAAAAATTAATG
  AAAGCTTAG
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  1809
  

⊟Protein

⊟General

• locus tag: SAUSA300_0902 [new locus tag: SAUSA300_RS04855 ]
• symbol: PepF
• description: oligoendopeptidase F
• length: 602
• theoretical pI: 4.92265
• theoretical MW: 69818.9
• GRAVY: -0.631063

⊟Function

• reaction:
  EC 3.4.24.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides oligoendopeptidase F (TIGR00181; EC 3.4.24.-; HMM-score: 669.1)
  and 2 more
  oligoendopeptidase, pepF/M3 family (TIGR02290; EC 3.4.24.-; HMM-score: 330.2)
  oligoendopeptidase, M3 family (TIGR02289; EC 3.4.24.-; HMM-score: 99.6)
• TheSEED  :
  • Oligoendopeptidase F
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M3; Peptidase family M3 (PF01432; HMM-score: 255)
  and 3 more
  no clan defined Peptidase_M3_N; Oligopeptidase F (PF08439; HMM-score: 72.5)
  SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 13)
  no clan defined DUF2264; Uncharacterized protein conserved in bacteria (DUF2264) (PF10022; HMM-score: 12.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002398
  • TAT(Tat/SPI): 0.00041
  • LIPO(Sec/SPII): 0.000373
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161444 NCBI
• RefSeq: YP_493602 NCBI
• UniProt: A0A0H2XIW1 UniProt

⊟Protein sequence

• MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGKEEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQLIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEKLLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLETDDRKLRESAFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLVKTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEPMGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVHEFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQELERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDISKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPIEILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0900 > SAUSA300_0901 > pepF

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications