PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2111 [new locus tag: SAUSA300_RS11630 ]
• pan locus tag^?: SAUPAN005506000
• symbol: glmM
• pan gene symbol^?: glmM
• synonym:
• product: phosphoglucosamine mutase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2111 [new locus tag: SAUSA300_RS11630 ]
• symbol: glmM
• product: phosphoglucosamine mutase
• replicon: chromosome
• strand: -
• coordinates: 2285970..2287325
• length: 1356
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913543 NCBI
• RefSeq: YP_494757 NCBI
• BioCyc: see SAUSA300_RS11630
• MicrobesOnline: 1293626 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGAAAATATTTTGGTACAGACGGAGTAAGAGGTGTCGCAAACCAAGAACTAACACCT
  GAATTGGCATTTAAATTAGGAAGATACGGTGGCTATGTTCTAGCACATAATAAAGGTGAA
  AAACACCCACGTGTACTTGTAGGTCGCGATACTAGAGTTTCAGGTGAAATGTTAGAATCA
  GCATTAATAGCTGGTTTGATTTCAATTGGTGCAGAAGTGATGCGATTAGGTATTATTTCA
  ACACCAGGTGTTGCATATTTAACACGCGATATGGGTGCAGAGTTAGGTGTAATGATTTCA
  GCCTCTCATAATCCAGTTGCAGATAATGGTATTAAATTCTTTGGATCAGATGGTTTTAAA
  CTATCAGATGAACAAGAAAATGAAATTGAAGCATTATTGGATCAAGAAAACCCAGAATTA
  CCAAGACCAGTTGGCAATGATATTGTACATTATTCAGATTACTTTGAAGGGGCACAAAAA
  TATTTGAGCTATTTAAAATCAACAGTAGATGTTAACTTTGAAGGTTTGAAAATTGCTTTA
  GATGGTGCAAATGGTTCAACATCATCACTAGCGCCATTCTTATTTGGTGACTTAGAAGCA
  GATACTGAAACAATTGGATGTAGTCCTGATGGATATAATATCAATGAGAAATGTGGCTCT
  ACACATCCTGAAAAATTAGCTGAAAAAGTAGTTGAAACTGAAAGTGATTTTGGGTTAGCA
  TTTGACGGCGATGGAGACAGAATCATAGCAGTAGATGAGAATGGTCAAATCGTTGACGGT
  GACCAAATTATGTTTATTATTGGTCAAGAAATGCATAAAAATCAAGAATTGAATAATGAC
  ATGATTGTTTCTACTGTTATGAGTAATTTAGGTTTTTACAAAGCGCTTGAACAAGAAGGA
  ATTAAATCTAATAAAACTAAAGTTGGCGACAGATATGTAGTAGAAGAAATGCGTCGCGGT
  AATTATAACTTAGGTGGAGAACAATCTGGACATATCGTTATGATGGATTACAATACAACT
  GGTGATGGTTTATTAACTGGTATTCAATTAGCTTCTGTAATAAAAATGACTGGTAAATCA
  CTAAGTGAATTAGCTGGACAAATGAAAAAATATCCACAATCATTAATTAACGTACGCGTA
  ACAGATAAATATCGTGTTGAAGAAAATGTTGACGTTAAAGAAGTTATGACTAAAGTAGAA
  GTAGAAATGAATGGAGAAGGTCGAATTTTAGTAAGACCTTCTGGAACAGAACCATTAGTT
  CGTGTCATGGTTGAAGCAGCAACTGATGAAGATGCTGAAAGATTTGCACAACAAATAGCT
  GATGTGGTTCAAGATAAAATGGGATTAGATAAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2111 [new locus tag: SAUSA300_RS11630 ]
• symbol: GlmM
• description: phosphoglucosamine mutase
• length: 451
• theoretical pI: 4.38454
• theoretical MW: 49265.2
• GRAVY: -0.319734

⊟Function

• reaction:
  EC 5.4.2.10?  ExPASy
  Phosphoglucosamine mutase Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
  Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR01455; EC 5.4.2.10; HMM-score: 605.7)
  and 3 more
  Metabolism Central intermediary metabolism Amino sugars phosphoglucosamine mutase (TIGR03990; EC 5.4.2.10; HMM-score: 346.6)
  Metabolism Energy metabolism Sugars phosphoglucomutase, alpha-D-glucose phosphate-specific (TIGR01132; EC 5.4.2.2; HMM-score: 26.8)
  Metabolism Energy metabolism Other 4-oxalocrotonate tautomerase family enzyme (TIGR00013; EC 5.3.2.-; HMM-score: 13.3)
• TheSEED  :
  • FemD, factor involved in methicillin resistance
  • Phosphoglucosamine mutase (EC 5.4.2.10)
  Cell Wall and Capsule Capsular and extracellular polysacchrides Sialic Acid Metabolism  Phosphoglucosamine mutase (EC 5.4.2.10)
  and 1 more
  Cell Wall and Capsule Cell Wall and Capsule - no subcategory UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis  Phosphoglucosamine mutase (EC 5.4.2.10)
• PFAM:
  no clan defined PGM_PMM_I; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I (PF02878; HMM-score: 147.6)
  and 4 more
  PGM_PMM_III; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III (PF02880; HMM-score: 114.1)
  PGM_PMM_IV; Phosphoglucomutase/phosphomannomutase, C-terminal domain (PF00408; HMM-score: 63.9)
  PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 57.7)
  Periplas_BP (CL0144) Peripla_BP_5; Periplasmic binding protein domain (PF13433; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Mg²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.015662
  • TAT(Tat/SPI): 0.00144
  • LIPO(Sec/SPII): 0.00086
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160822 NCBI
• RefSeq: YP_494757 NCBI
• UniProt: Q2FEX1 UniProt

⊟Protein sequence

• MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPRVLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISASHNPVADNGIKFFGSDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQKYLSYLKSTVDVNFEGLKIALDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKCGSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQELNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVMMDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDVKEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: glmM < SAUSA300_2112 < SAUSA300_2113

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications