PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
• pan locus tag^?: SAUPAN005886000
• symbol: SAUSA300_2317
• pan gene symbol^?: —
• synonym:
• product: putative zinc-binding dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
• symbol: SAUSA300_2317
• product: putative zinc-binding dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 2491405..2492307
• length: 903
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3912941 NCBI
• RefSeq: YP_494952 NCBI
• BioCyc: see SAUSA300_RS12800
• MicrobesOnline: 1293832 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  GTGCTGATTAAAGTACATTACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGAT
  CATAATGCAGTCGTAAAATCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATT
  GTTGAATCCGAAGCACCAGGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGAC
  CTAGGTGTCAGCCATTATGGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATT
  ATCAAGCTTCCTGATACTTTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTAT
  ACTGCCGGTTTAGCAATTGAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCT
  GTACTCGTTCGCGGTGCTTCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAA
  CTTGGTTATAAAGTTATCGCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAA
  CTTGGTGCCAAAGAAGTTATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTC
  GCATCATCAACTTGGCAAGCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTT
  ACAAAGCGTTTAAATCATAGTGGGTCAATTGCAGTTATTGGTATGACTGCCGGTAATACT
  TATACTAATTCTGTATTCCCTCACATTTTAAGAGGTGTAAACATTTTAGGAATTGACTCG
  GTATTTACTGCTATGAAATTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAATG
  CCTGAAAATTTACATGAGATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTT
  AACAAAGTAATTAAACATGAAAATAAAGGGCGCATTGTTATCGATTTCGGTGTAGATAAA
  TAG
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  903
  

⊟Protein

⊟General

• locus tag: SAUSA300_2317 [new locus tag: SAUSA300_RS12800 ]
• symbol: SAUSA300_2317
• description: putative zinc-binding dehydrogenase
• length: 300
• theoretical pI: 5.87292
• theoretical MW: 32835.5
• GRAVY: -0.0753333

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 422.5)
  and 7 more
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 89.7)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 51.3)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 35.9)
  leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.7)
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 25.5)
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 25)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 18.1)
• TheSEED  :
  • Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84)
  Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
  and 2 more
  Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
• PFAM:
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 47.1)
  and 3 more
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 30.1)
  NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 15.7)
  ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.012456
  • TAT(Tat/SPI): 0.000311
  • LIPO(Sec/SPII): 0.001529
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160438 NCBI
• RefSeq: YP_494952 NCBI
• UniProt: A0A0H2XF07 UniProt

⊟Protein sequence

• MLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications