PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA2160 [new locus tag: SA_RS12400 ]
• pan locus tag^?: SAUPAN005886000
• symbol: SA2160
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA2160 [new locus tag: SA_RS12400 ]
• symbol: SA2160
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 2427313..2428317
• length: 1005
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1125088 NCBI
• RefSeq: NP_375483 NCBI
• BioCyc: see SA_RS12400
• MicrobesOnline: 104509 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  GTGATAGAAACATTTAAAGCGTTTGTAATTGATAAAGATGAGAGCGGTAAAGTGACACCA
  ACTTTCAAACAATTATCGCCTACTGATTTACCTAAAGGAGATGTGCTGATTAAAGTACAT
  TACTCTGGTATAAATTATAAAGATGCTTTAGCGACTCAAGATCATAATGCAGTCGTAAAA
  TCGTATCCTATGATTCCAGGAATAGATTTAGCTGGAACAATTGTTGAATCCGAAGCACCA
  GGCTTTGAAAAAGGAGAACAAGTAATTGTAACGAGTTATGACCTAGGTGTTAGCCATTAT
  GGCGGTTTTAGTGAATATGCGCGTGTAAAATCAGAATGGATTATCAAGCTTCCTGATACT
  TTAACATTAGAAGAATCAATGATATATGGCACAGCTGGTTATACTGCCGGTTTAGCAATT
  GAAAGACTTGAAAAAGTTGGAATGAATATTGAAGATGGTCCTGTACTCGTTCGCGGTGCT
  TCAGGTGGTGTCGGTACTTTAGCAGTACTCATGCTTAATGAACTTGGTTATAAAGTTATC
  GCAAGTACAGGTAAACAAGATGTTAGCGATCAATTACTTGAACTTGGTGCCAAAGAAGTT
  ATCGATCGACTTCCTGTTGAAGATGATCATAAAAAGCCACTCGCATCATCAACTTGGCAA
  GCTTGTGTAGACCCTGTTGGTGGCGAAGGTATTAATTATGTTACAAAACGTTTAAATCAT
  AGTGGATCAATTGCAGTTATTGGTATGACTGCCGGCAATACTTATACTAATTCTGTATTC
  CCTCACATTTTAAGAGGCGTAAACATTTTAGGAATTGACTCAGTATTTACTGCTATGAAA
  TTAAGACAGCGCGTTTGGCGTCGTCTCGCAAAAGATTTAAAGCCTGAAAATTTACATGAG
  ATCAAGCAAGTTATTACATTTGATGAACTTCCAGAACAACTTAACAAAGTAATTAATCAT
  GAAAATAAAGGCCGCATTGTTATCGATTTCGGTGTAGATAAATAG
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⊟Protein

⊟General

• locus tag: SA2160 [new locus tag: SA_RS12400 ]
• symbol: SA2160
• description: hypothetical protein
• length: 334
• theoretical pI: 5.65303
• theoretical MW: 36552.6
• GRAVY: -0.127545

⊟Function

• TIGRFAM:
  Unknown function Enzymes of unknown specificity putative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 452)
  and 8 more
  Unknown function Enzymes of unknown specificity putative NAD(P)H quinone oxidoreductase, PIG3 family (TIGR02824; HMM-score: 95.8)
  crotonyl-CoA carboxylase/reductase (TIGR01751; EC 1.3.1.85; HMM-score: 57.8)
  Metabolism Energy metabolism Fermentation zinc-binding alcohol dehydrogenase family protein (TIGR02817; HMM-score: 38.5)
  leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase (TIGR02825; EC 1.3.1.48,1.3.1.74; HMM-score: 35.4)
  Unknown function Enzymes of unknown specificity NDMA-dependent alcohol dehydrogenase, Rxyl_3153 family (TIGR03989; EC 1.1.99.36; HMM-score: 30.9)
  Metabolism Energy metabolism Amino acids and amines L-threonine 3-dehydrogenase (TIGR00692; EC 1.1.1.103; HMM-score: 26.9)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll chlorophyll synthesis pathway protein BchC (TIGR01202; HMM-score: 25.2)
  6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase (TIGR03201; EC 1.1.1.-; HMM-score: 12.8)
• TheSEED  :
  • Acryloyl-CoA reductase AcuI/YhdH (EC 1.3.1.84)
  Carbohydrates Fermentation Fermentations: Lactate  Alcohol dehydrogenase (EC 1.1.1.1)
  and 2 more
  Carbohydrates Fermentation Fermentations: Mixed acid  Alcohol dehydrogenase (EC 1.1.1.1)
  Fatty Acids, Lipids, and Isoprenoids Phospholipids Glycerolipid and Glycerophospholipid Metabolism in Bacteria  Alcohol dehydrogenase (EC 1.1.1.1)
• PFAM:
  NADP_Rossmann (CL0063) ADH_zinc_N; Zinc-binding dehydrogenase (PF00107; HMM-score: 46.7)
  and 4 more
  GroES (CL0296) ADH_N; Alcohol dehydrogenase GroES-like domain (PF08240; HMM-score: 34.5)
  NADP_Rossmann (CL0063) ADH_zinc_N_2; Zinc-binding dehydrogenase (PF13602; HMM-score: 16.6)
  GroES (CL0296) ADH_N_2; N-terminal domain of oxidoreductase (PF16884; HMM-score: 13.6)
  NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004128
  • TAT(Tat/SPI): 0.000424
  • LIPO(Sec/SPII): 0.000657
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927950 NCBI
• RefSeq: NP_375483 NCBI
• UniProt: A0A0H3JMJ3 UniProt

⊟Protein sequence

• MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGINYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGGFSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGASGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASSTWQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVFTAMKLRQRVWRRLAKDLKPENLHEIKQVITFDELPEQLNKVINHENKGRIVIDFGVDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications