From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2541 [new locus tag: SAUSA300_RS14105 ]
  • pan locus tag?: SAUPAN006306000
  • symbol: mqo
  • pan gene symbol?: lqo
  • synonym:
  • product: malate:quinone oxidoreductase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2541 [new locus tag: SAUSA300_RS14105 ]
  • symbol: mqo
  • product: malate:quinone oxidoreductase
  • replicon: chromosome
  • strand: -
  • coordinates: 2744110..2745606
  • length: 1497
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCTAAGTCTAATAGTAAAGACATCGTTTTAATTGGAGCCGGTGTACTTAGCACAACA
    TTTGGTTCAATGTTAAAAGAAATTGAGCCAGACTGGAATATCCACGTTTACGAACGCTTG
    GATCGTCCTGCAATCGAAAGTTCAAACGAAAGAAATAATGCTGGTACGGGTCATGCAGCA
    TTATGTGAGTTGAACTACACAGTTTTACAACCTGATGGTTCTATCGACATCGAAAAAGCG
    AAAGTGATTAACGAAGAGTTTGAGATTTCAAAACAATTCTGGGGTCACTTAGTGAAAAGC
    GGTAGCATCGAGAACCCAAGAGAATTTATCAATCCATTACCACACATCAGTTATGTTAGA
    GGTAAAAACAATGTTAAATTCTTAAAAGATCGTTACGAAGCGATGAAAGCTTTCCCTATG
    TTCGATAATATCGAATATACTGAAGACATCGAAGTAATGAAAAAATGGATTCCATTGATG
    ATGAAAGGCCGTGAAGATAACCCTGGTATCATGGCGGCAAGTAAAATTGACGAAGGTACA
    GATGTAAACTTCGGTGAATTAACACGTAAAATGGCTAAAAGCATTGAAGCACATCCAAAT
    GCTACAGTGCAATTTAACCATGAAGTTGTTGATTTTGAACAATTATCAAATGGTCAATGG
    GAAGTTACTGTTAAAAATCGCCTAACTGGTGAGAAATTCAAACAAGTAACTGACTACGTA
    TTCATCGGTGCTGGCGGTGGAGCAATTCCATTATTACAAAAAACAGGTATCCCTGAAAGT
    AAACATTTGGGTGGATTCCCTATCAGTGGTCAATTCTTAGCTTGTACAAACCCACAAGTT
    ATTGAACAACACGATGCCAAAGTTTATGGTAAAGAGCCACCTGGTACACCACCAATGACT
    GTACCTCATTTAGATACGCGTTACATTGATGGTCAAAGAACATTATTATTTGGACCATTT
    GCTAATGTTGGACCTAAATTCTTGAAAAATGGTTCTAACTTAGATTTATTCAAGTCTGTT
    AAAACATACAACATTACAACTTTATTAGCAGCAGCAGTTAAAAACTTACCTTTAATTAAA
    TACTCATTTGACCAAGTAATTATGACAAAAGAAGGTTGTATGAACCACTTACGTACTTTC
    TATCCAGAAGCACGTAATGAAGATTGGCAATTATACACTGCTGGTAAACGTGTACAAGTT
    ATCAAAGATACACCTGAACACGGTAAAGGATTCATCCAATTCGGTACAGAAGTGGTTAAC
    TCACAAGACCACACTGTAATTGCATTATTAGGTGAATCACCAGGGGCTTCAACTTCAGTT
    TCAGTTGCGTTAGAAGTATTAGAACGTAACTTCCCTGAATACAAAACTGAATGGGCACCT
    AAAATTAAGAAAATGATTCCATCATACGGTGAATCATTAATTGAAGACGAAAAATTAATG
    AGAAAAATCCGTAAACAAACTTCAAAAGACTTAGAATTAGGTTACTACGAAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1497

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2541 [new locus tag: SAUSA300_RS14105 ]
  • symbol: Mqo
  • description: malate:quinone oxidoreductase
  • length: 498
  • theoretical pI: 6.52415
  • theoretical MW: 55998.5
  • GRAVY: -0.430924

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.5.4?  ExPASy
    Malate dehydrogenase (quinone) (S)-malate + a quinone = oxaloacetate + reduced quinone
  • TIGRFAM:
    Metabolism Energy metabolism TCA cycle malate dehydrogenase (acceptor) (TIGR01320; EC 1.1.5.4; HMM-score: 633.5)
    and 2 more
    Cellular processes Cellular processes Detoxification CoA-disulfide reductase (TIGR03385; EC 1.8.1.14; HMM-score: 16.2)
    conserved hypothetical protein (TIGR04540; HMM-score: 12.8)
  • TheSEED  :
    • Malate dehydrogenase (quinone) (EC 1.1.5.4)
    Carbohydrates Central carbohydrate metabolism TCA Cycle  Malate:quinone oxidoreductase (EC 1.1.5.4)
  • PFAM:
    NADP_Rossmann (CL0063) Mqo; Malate:quinone oxidoreductase (Mqo) (PF06039; HMM-score: 719.5)
    and 2 more
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 22.9)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 13)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FAD
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 0.53
    • Cellwall Score: 8.75
    • Extracellular Score: 0.7
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.093464
    • TAT(Tat/SPI): 0.007823
    • LIPO(Sec/SPII): 0.022182
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPAIESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGSIENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLMMKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNGQWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACTNPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNLDLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLYTAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERNFPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_1540(dnaK)molecular chaperone DnaK  [1] (data from MRSA252)
    SAUSA300_0532(fusA)elongation factor G  [1] (data from MRSA252)
    SAUSA300_1644(pyk)pyruvate kinase  [1] (data from MRSA252)
    SAUSA300_0523(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SAUSA300_2192(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SAUSA300_0524(rplJ)50S ribosomal protein L10  [1] (data from MRSA252)
    SAUSA300_0522(rplK)50S ribosomal protein L11  [1] (data from MRSA252)
    SAUSA300_2202(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_053130S ribosomal protein S7  [1] (data from MRSA252)
    SAUSA300_2484hydroxymethylglutaryl-CoA synthase  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_2541&oldid=98561"