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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00435
  • pan locus tag?: SAUPAN002186000
  • symbol: SAOUHSC_00435
  • pan gene symbol?: gltB
  • synonym:
  • product: glutamate synthase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00435
  • symbol: SAOUHSC_00435
  • product: glutamate synthase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 433332..437831
  • length: 4500
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
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    121
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    481
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    601
    661
    721
    781
    841
    901
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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCACAATGAGAAATTAATTAAAGGCTTATATGACTATCGTGAGGAACATGATGCGTGT
    GGTATTGGTTTTTATGCGAATATGGATAATAAAAGGTCTCACGACATCATTGATAAATCG
    CTTGAAATGTTGCGACGCTTAGATCACAGGGGCGGGGTCGGCGCAGATGGCATCACTGGT
    GATGGCGCAGGTATTATGACTGAAATACCTTTTGCATTTTTCAAACAACATGTAACGGAC
    TTTGATATCCCAGGTGAAGGTGAATATGCCGTGGGGTTATTTTTTTCCAAAGAACGCATT
    TTAGGTTCTGAACATGAAGTAGTTTTTAAAAAATATTTTGAAGGCGAAGGGTTATCAATT
    CTTGGTTATCGTAATGTACCAGTTAATAAAGATGCCATTGCTAAACATGTAGCAGATACG
    ATGCCAGTCATTCAACAAGTGTTTATTGATATTAGGGACATTGAAGATGTTGAAAAGCGT
    TTGTTTTTAGCGAGAAAACAATTAGAGTTCTATTCGACTCAGTGCGATTTAGAATTGTAT
    TTTACGAGCTTATCACGCAAAACAATTGTATATAAAGGTTGGTTACGATCAGACCAAATT
    AAAAAACTATATACAGATTTATCGGATGATTTATATCAATCAAAGCTAGGGTTAGTGCAT
    TCGAGATTTAGTACGAATACATTCCCGAGTTGGAAAAGGGCACATCCTAACCGTATGTTA
    ATGCATAATGGTGAGATTAACACGATTAAAGGTAATGTAAACTGGATGCGAGCACGCCAA
    CATAAATTAATCGAAACATTATTTGGCGAGGATCAACATAAAGTGTTTCAAATTGTCGAT
    GAGGATGGTAGTGACTCTGCCATTGTAGATAATGCGCTAGAGTTCTTATCGTTAGCCATG
    GAGCCAGAAAAGGCAGCGATGTTACTCATACCTGAACCTTGGTTATATAATGAAGCGAAT
    GATGCAAATGTACGTGCGTTTTATGAATTTTATAGTTATTTAATGGAACCGTGGGATGGT
    CCTACAATGATTTCGTTCTGTAACGGTGACAAACTTGGCGCGCTTACAGATAGAAATGGA
    TTACGTCCAGGTCGTTATACGATTACTAAAGATAACTTTATTGTCTTTTCATCTGAAGTG
    GGTGTTGTGGACGTACCTGAAAGTAATGTTGCTTTTAAAGGTCAATTGAATCCTGGAAAG
    TTATTGCTTGTTGATTTTAAACAGAATAAAGTCATTGAAAATAATGATTTAAAAGGTGCG
    ATTGCTGGAGAATTACCATATAAAGCGTGGATTGATAACCATAAAGTTGACTTTGATTTT
    GAAAATATACAATATCAAGATTCGCAATGGAAAGATGAGACGTTATTTAAATTACAACGT
    CAGTTTGCATACACGAAAGAAGAGATTCATAAGTATATTCAGGAACTTGTAGAAGGTAAG
    AAGGATCCTATCGGTGCAATGGGATATGATGCGCCAATTGCAGTGTTGAACGAGCGACCA
    GAATCACTATTTAATTACTTTAAACAGCTGTTTGCACAAGTTACGAATCCACCAATTGAT
    GCGTATCGTGAAAAAATCGTAACGAGTGAACTTTCTTATTTAGGTGGCGAAGGTAACTTA
    CTAGCACCTGACGAAACGGTTTTAGATCGTATTCAATTGAAAAGGCCGGTATTGAATGAA
    TCACACTTAGCAGCGATTGATCAGGAACATTTTAAATTAACTTATTTATCAACGGTATAT
    GAAGGGGATTTGGAAGATGCGTTAGAAGCATTAGGCCGAGAAGCAGTGAATGCTGTAAAG
    CAAGGCGCTCAAATTCTAGTGTTAGATGATAGTGGATTAGTTGATAGCAATGGCTTTGCA
    ATGCCGATGTTACTCGCAATAAGTCATGTGCATCAATTACTTATTAAAGCAGATTTACGT
    ATGTCTACAAGTTTAGTCGCTAAATCTGGTGAGACACGAGAAGTGCATCATGTTGCTTGT
    TTACTCGCATATGGCGCGAATGCAATTGTGCCATACCTAGCGCAACGTACAGTTGAACAA
    CTGACATTGACAGAAGGGTTACAAGGCACCGTTGTCGATAATGTTAAGACATATACGGAT
    GTATTGTCAGAAGGTGTCATTAAAGTAATGGCTAAGATGGGAATTTCGACAGTGCAAAGT
    TATCAAGGGGCACAAATATTTGAAGCGATTGGCTTGTCTCATGATGTGATTGATCGTTAT
    TTTACTGGGACACAGTCTAAGTTATCTGGTATTTCGATTGATCAAATTGATGCTGAAAAT
    AAAGCACGTCAACAAAGTGATGATAATTATCTTGCATCAGGTAGTACATTCCAATGGAGA
    CAACAAGGTCAACATCATGCTTTTAATCCGGAATCTATTTTCTTATTGCAGCACGCATGT
    AAAGAAAATGACTATGCGCAATTTAAAGCATACTCTGAAGCGGTGAACAAAAATAGAACA
    GATCACATTAGACATTTACTTGAATTTAAAGCATGTACACCGATTGACATCGACCAAGTT
    GAACCGGTAAGTGACATTGTCAAACGCTTTAATACAGGGGCGATGAGTTATGGATCGATT
    TCAGCGGAAGCACATGAAACGTTAGCACAAGCCATGAACCAATTAGGTGGAAAGAGTAAT
    AGTGGTGAAGGTGGCGAAGATGCAAAACGTTATGAAGTACAAGTTGATGGAAGCAACAAA
    GTAAGTGCGATTAAACAAGTTGCTTCTGGGCGTTTTGGTGTAACTAGTGATTATTTACAA
    CATGCCAAAGAAATTCAAATTAAAGTTGCGCAAGGTGCAAAGCCTGGTGAAGGTGGTCAA
    TTACCTGGTACTAAGGTATATCCGTGGATTGCGAAGACAAGAGGGTCAACGCCAGGTATC
    GGTCTGATTTCACCACCGCCACATCATGATATTTATTCAATAGAAGATTTAGCGCAACTG
    ATACATGATTTGAAAAATGCGAATAAAGATGCAGATATCGCGGTAAAATTAGTTTCGAAA
    ACAGGTGTTGGTACCATTGCATCTGGGGTGGCAAAAGCATTTGCAGATAAAATTGTCATC
    AGTGGTTACGATGGTGGTACAGGGGCTTCACCCAAAACGAGTATTCAGCATGCCGGTGTT
    CCTTGGGAGATTGGTTTAGCAGAAACACATCAAACATTAAAACTAAATGACTTAAGAAGT
    CGTGTTAAGTTAGAAACAGACGGTAAGTTATTAACTGGTAAAGATGTAGCGTACGCATGT
    GCGCTTGGAGCGGAAGAATTTGGATTTGCAACTGCACCATTAGTGGTGTTGGGCTGTATT
    ATGATGCGTGTATGCCATAAAGATACATGTCCAGTAGGAGTTGCAACTCAAAACAAAGAT
    TTACGTGCTTTATATAGAGGTAAAGCACATCATGTTGTTAATTTTATGCATTTTATTGCA
    CAAGAATTAAGAGAAATTTTAGCATCTTTAGGTTTGAAACGTGTAGAAGACTTAGTTGGA
    AGAACTGATTTATTACAACGATCATCAACATTAAAAGCGAATAGCAAAGCGGCTAGTATT
    GATGTTGAAAAACTGTTATGTCCTTTCGATGGGCCAAACACAAAAGAAATTCAACAAAAT
    CATAATCTTGAGCATGGATTTGATTTAACAAATTTATATGAAGTAACGAAGCCATATATT
    GCTGAAGGGCGTCGCTATACAGGTAGCTTTACAGTAAATAATGAACAACGTGATGTAGGG
    GTTATTACAGGTAGTGAGATTTCGAAACAATATGGAGAAGCAGGACTTCCTGAAAATACA
    ATTAATGTTTATACGAATGGTCATGCTGGTCAAAGTCTTGCAGCATATGCACCGAAAGGC
    TTAATGATTCATCATACTGGAGATGCGAATGACTATGTTGGTAAAGGATTATCTGGTGGT
    ACGGTCATTGTCAAAGCACCTTTTGAAGAACGACAAAATGAAATTATTGCTGGTAACGTC
    TCATTCTATGGTGCGACAAGTGGTAAGGCATTTATTAACGGTAGTGCAGGAGAAAGATTC
    TGTATTAGAAATAGTGGTGTAGATGTTGTCGTTGAAGGTATCGGCGACCATGGATTAGAG
    TATATGACTGGTGGACATGTCATTAATTTAGGTGATGTAGGTAAGAACTTCGGTCAAGGT
    ATGAGTGGTGGTATTGCTTACGTTATCCCGTCTGATGTAGAAGCTTTTGTTGAAAATAAT
    CAACTAGATACGCTTTCGTTTACAAAGATTAAACACCAAGAAGAAAAAGCATTCATTAAG
    CAAATGCTGGAAGAACATGTGTCACACACGAATAGTACGAGAGCGATTCATGTGTTAAAA
    CATTTTGATCGCATTGAAGATGTCGTCGTTAAAGTTATTCCTAAAGATTATCAATTAATG
    ATGCAAAAAATTCATTTGCACAAATCATTACATGACAATGAAGATGAAGCGATGTTAGCT
    GCATTTTACGATGACAGTAAAACAATCGATGCTAAACATAAACCAGCCGTTGTGTATTAA
    60
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    2160
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    3180
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    4140
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    4260
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    4500

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00435
  • symbol: SAOUHSC_00435
  • description: glutamate synthase large subunit
  • length: 1499
  • theoretical pI: 5.97235
  • theoretical MW: 166347
  • GRAVY: -0.316211

⊟Function[edit | edit source]

  • reaction:
    EC 1.4.1.13?  ExPASy
    Glutamate synthase (NADPH) 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH
  • TIGRFAM:
    formylmethanofuran dehydrogenase subunit C (TIGR03122; EC 1.2.99.5; HMM-score: 25.9)
  • TheSEED  :
    • Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
    Amino Acids and Derivatives Glutamine, glutamate, aspartate, asparagine; ammonia assimilation Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis  Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
    and 1 more
    Nitrogen Metabolism Nitrogen Metabolism - no subcategory Ammonia assimilation  Glutamate synthase [NADPH] large chain (EC 1.4.1.13)
  • PFAM:
    TIM_barrel (CL0036) Glu_synthase; Conserved region in glutamate synthase (PF01645; HMM-score: 521.3)
    NTN (CL0052) GATase_2; Glutamine amidotransferases class-II (PF00310; HMM-score: 514.1)
    and 3 more
    TIM_barrel (CL0036) Glu_syn_central; Glutamate synthase central domain (PF04898; HMM-score: 330.4)
    no clan defined GXGXG; GXGXG motif (PF01493; HMM-score: 225.1)
    TIM_barrel (CL0036) FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 9.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.78
    • Cytoplasmic Membrane Score: 8.16
    • Cellwall Score: 0.06
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.029572
    • TAT(Tat/SPI): 0.000731
    • LIPO(Sec/SPII): 0.001237
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEMLRRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERILGSEHEVVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKRLFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLGLVHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVFQIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYLMEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAFKGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQWKDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFKQLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAIDQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPMLLAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLTLTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRYFTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQHACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMSYGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVASGRFGVTSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIYSIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGASPKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFGFATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREILASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLEHGFDLTNLYEVTKPYIAEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTINVYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNVSFYGATSGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFGQGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAIHVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHKPAVVY

⊟Experimental data[edit | edit source]

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: GltC* (activation) regulon, CodY* (repression) regulon
    GltC*(TF)important in Glutamate metabolism; RegPrecise 
    CodY*(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3.20 3.21 3.22 3.23 3.24 3.25 3.26 3.27 3.28 3.29 3.30 3.31 3.32 3.33 3.34 3.35 3.36 3.37 3.38 3.39 3.40 3.41 3.42 3.43 3.44 3.45 3.46 3.47 3.48 3.49 3.50 3.51 3.52 3.53 3.54 3.55 3.56 3.57 3.58 3.59 3.60 3.61 3.62 3.63 3.64 3.65 3.66 3.67 3.68 3.69 3.70 3.71 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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