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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS07665 [old locus tag: SACOL1505 ]
- pan locus tag?: SAUPAN003925000
- symbol: SACOL_RS07665
- pan gene symbol?: aroB
- synonym:
- product: 3-dehydroquinate synthase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS07665 [old locus tag: SACOL1505 ]
- symbol: SACOL_RS07665
- product: 3-dehydroquinate synthase
- replicon: chromosome
- strand: -
- coordinates: 1545985..1547049
- length: 1065
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGAAATTACAAACAACATACCCTTCAAATAATTATCCAATATATGTTGAACACGGTGCA
ATTGACCATATTAGCACGTATATTGATCAGTTTGATCAAAGTTTTATATTAATTGACGAG
CATGTAAATCAATATTTTGCTGATAAATTTGATGATATTTTATCATATGAAAATGTACAT
AAAGTTATTATTCCAGCTGGTGAAAAGACGAAAACATTTGAGCAATATCAAGAAACATTA
GAGTATATTTTATCCCATCATGTAACTCGTAATACAGCAATTATTGCTGTTGGTGGTGGT
GCAACTGGTGATTTTGCTGGGTTTATTGCGGCGACACTTTTACGAGGCGTTCACTTTATA
CAAGTGCCAACGACTATACTAGCGCATGATTCTAGTGTTGGCGGTAAAGTGGGTATTAAC
TCAAAGCAAGGTAAAAACCTTATCGGTGCATTTTATCGTCCAACTGCTGTGATTTATGAT
TTAGACTTTTTAAAGACGTTACCATTTGAGCAAATATTAAGTGGCTATGCAGAAGTTTAT
AAGCATGCGTTATTGAATGGTGAATCAGCGACGCAAGATATCGAACAGCACTTTAAAGAT
AGAGAGATATTACAGTCATTAAATGGTATGGATAAATATATTGCTAAAGGTATTGAAACG
AAGCTGGATATTGTTATTGCAGATGAAAAAGAACAAGGTGTACGTAAATTTTTAAATTTA
GGTCATACATTTGGTCATGCTGTTGAATACTATCATAAAATACCTCATGGTCATGCAGTG
ATGGTTGGCATTATCTATCAATTTATAGTTGCGAATGCTTTGTTTGATTCTAAGCATGAT
ATTAATCATTATATTCAATATTTAATACAACTCGGCTATCCTTTAGACATGATAACTGAC
TTGGATTTTGAAACGTTATACCAATATATGCTAAGTGATAAAAAGAATGATAAGCAAGGT
GTACAAATGGTCTTGATTAGACAATTTGGAGATATCGTTGTACAACATGTTGATCAACTA
ACATTACAACATGCATGTGAACAATTAAAAACATATTTTAAGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS07665 [old locus tag: SACOL1505 ]
- symbol: SACOL_RS07665
- description: 3-dehydroquinate synthase
- length: 354
- theoretical pI: 6.09151
- theoretical MW: 40315.6
- GRAVY: -0.156497
⊟Function[edit | edit source]
- reaction: EC 4.2.3.4? ExPASy3-dehydroquinate synthase 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate
- TIGRFAM: Amino acid biosynthesis Aromatic amino acid family 3-dehydroquinate synthase (TIGR01357; EC 4.2.3.4; HMM-score: 376)and 1 moreBiosynthesis of cofactors, prosthetic groups, and carriers Lipoate putative sugar phosphate phospholyase (cyclizing) (TIGR04425; EC 4.2.3.-; HMM-score: 235.9)
- TheSEED: see SACOL1505
- PFAM: DHQS (CL0224) DHQ_synthase; 3-dehydroquinate synthase (PF01761; HMM-score: 289.9)and 2 moreFe-ADH_2; Iron-containing alcohol dehydrogenase (PF13685; HMM-score: 59.4)Fe-ADH; Iron-containing alcohol dehydrogenase (PF00465; HMM-score: 52.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Co2+, NAD+, Zn2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00262
- TAT(Tat/SPI): 0.000078
- LIPO(Sec/SPII): 0.000281
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKLQTTYPSNNYPIYVEHGAIDHISTYIDQFDQSFILIDEHVNQYFADKFDDILSYENVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGATGDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPTAVIYDLDFLKTLPFEQILSGYAEVYKHALLNGESATQDIEQHFKDREILQSLNGMDKYIAKGIETKLDIVIADEKEQGVRKFLNLGHTFGHAVEYYHKIPHGHAVMVGIIYQFIVANALFDSKHDINHYIQYLIQLGYPLDMITDLDFETLYQYMLSDKKNDKQGVQMVLIRQFGDIVVQHVDQLTLQHACEQLKTYFK
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL1505
- protein localization: see SACOL1505
- quantitative data / protein copy number per cell: see SACOL1505
- interaction partners:
SACOL_RS00095 50S ribosomal protein L9 [1] (data from MRSA252) SACOL_RS00470 peptidoglycan-binding protein LysM [1] (data from MRSA252) SACOL_RS01040 formate acetyltransferase [1] (data from MRSA252) SACOL_RS01135 L-lactate dehydrogenase [1] (data from MRSA252) SACOL_RS02210 30S ribosomal protein S18 [1] (data from MRSA252) SACOL_RS02285 alkyl hydroperoxide reductase subunit C [1] (data from MRSA252) SACOL_RS02330 IMP dehydrogenase [1] (data from MRSA252) SACOL_RS02640 YbaB/EbfC family nucleoid-associated protein [1] (data from MRSA252) SACOL_RS02790 50S ribosomal protein L25/general stress protein Ctc [1] (data from MRSA252) SACOL_RS02850 cysteine synthase [1] (data from MRSA252) SACOL_RS02930 pyridoxal 5'-phosphate synthase lyase subunit PdxS [1] (data from MRSA252) SACOL_RS03025 50S ribosomal protein L11 [1] (data from MRSA252) SACOL_RS03035 50S ribosomal protein L10 [1] (data from MRSA252) SACOL_RS03040 50S ribosomal protein L7/L12 [1] (data from MRSA252) SACOL_RS03050 DNA-directed RNA polymerase subunit beta [1] (data from MRSA252) SACOL_RS03055 DNA-directed RNA polymerase subunit beta' [1] (data from MRSA252) SACOL_RS03070 30S ribosomal protein S7 [1] (data from MRSA252) SACOL_RS03075 elongation factor G [1] (data from MRSA252) SACOL_RS03080 elongation factor Tu [1] (data from MRSA252) SACOL_RS03275 phosphate acetyltransferase [1] (data from MRSA252) SACOL_RS03415 zinc-dependent alcohol dehydrogenase [1] (data from MRSA252) SACOL_RS03540 metal ABC transporter substrate-binding protein [1] (data from MRSA252) SACOL_RS04310 aldehyde dehydrogenase [1] (data from MRSA252) SACOL_RS04315 phosphoglycerate kinase [1] (data from MRSA252) SACOL_RS04320 triose-phosphate isomerase [1] (data from MRSA252) SACOL_RS04330 enolase [1] (data from MRSA252) SACOL_RS04775 TIGR01457 family HAD-type hydrolase [1] (data from MRSA252) SACOL_RS04840 NADH dehydrogenase [1] (data from MRSA252) SACOL_RS04925 NAD-specific glutamate dehydrogenase [1] (data from MRSA252) SACOL_RS04950 glucose-6-phosphate isomerase [1] (data from MRSA252) SACOL_RS04980 hypothetical protein [1] (data from MRSA252) SACOL_RS05205 hypothetical protein [1] (data from MRSA252) SACOL_RS05375 1,4-dihydroxy-2-naphthoyl-CoA synthase [1] (data from MRSA252) SACOL_RS05575 phosphoenolpyruvate--protein phosphotransferase [1] (data from MRSA252) SACOL_RS05630 pyruvate dehydrogenase E1 component subunit alpha [1] (data from MRSA252) SACOL_RS05635 pyruvate dehydrogenase E1 component subunit beta [1] (data from MRSA252) SACOL_RS05640 dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex [1] (data from MRSA252) SACOL_RS05645 dihydrolipoyl dehydrogenase [1] (data from MRSA252) SACOL_RS05900 thiol reductase thioredoxin [1] (data from MRSA252) SACOL_RS06140 cell division protein FtsZ [1] (data from MRSA252) SACOL_RS06375 acyl carrier protein [1] (data from MRSA252) SACOL_RS06405 30S ribosomal protein S16 [1] (data from MRSA252) SACOL_RS06420 50S ribosomal protein L19 [1] (data from MRSA252) SACOL_RS06445 succinyl-CoA ligase subunit beta [1] (data from MRSA252) SACOL_RS06495 GTP-sensing pleiotropic transcriptional regulator CodY [1] (data from MRSA252) SACOL_RS06505 30S ribosomal protein S2 [1] (data from MRSA252) SACOL_RS06515 elongation factor Ts [1] (data from MRSA252) SACOL_RS06770 glutamine synthetase [1] (data from MRSA252) SACOL_RS07010 transketolase [1] (data from MRSA252) SACOL_RS07050 aconitate hydratase [1] (data from MRSA252) SACOL_RS07385 dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex [1] (data from MRSA252) SACOL_RS07920 phosphogluconate dehydrogenase (NADP(+)-dependent, decarboxylating) [1] (data from MRSA252) SACOL_RS08115 glycine dehydrogenase [1] (data from MRSA252) SACOL_RS08210 superoxide dismutase [1] (data from MRSA252) SACOL_RS08270 glycine--tRNA ligase [1] (data from MRSA252) SACOL_RS08345 molecular chaperone DnaK [1] (data from MRSA252) SACOL_RS08670 50S ribosomal protein L27 [1] (data from MRSA252) SACOL_RS08680 50S ribosomal protein L21 [1] (data from MRSA252) SACOL_RS08795 trigger factor [1] (data from MRSA252) SACOL_RS08820 50S ribosomal protein L20 [1] (data from MRSA252) SACOL_RS08825 50S ribosomal protein L35 [1] (data from MRSA252) SACOL_RS08865 aldehyde dehydrogenase [1] (data from MRSA252) SACOL_RS08905 isocitrate dehydrogenase (NADP(+)) [1] (data from MRSA252) SACOL_RS08910 citrate synthase [1] (data from MRSA252) SACOL_RS08930 pyruvate kinase [1] (data from MRSA252) SACOL_RS09125 formate--tetrahydrofolate ligase [1] (data from MRSA252) SACOL_RS09225 D-alanine aminotransferase [1] (data from MRSA252) SACOL_RS09385 transaldolase [1] (data from MRSA252) SACOL_RS10210 non-heme ferritin [1] (data from MRSA252) SACOL_RS10250 aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B [1] (data from MRSA252) SACOL_RS10530 molecular chaperone GroEL [1] (data from MRSA252) SACOL_RS10535 co-chaperone GroES [1] (data from MRSA252) SACOL_RS10760 anti-sigma B factor antagonist [1] (data from MRSA252) SACOL_RS11010 uracil phosphoribosyltransferase [1] (data from MRSA252) SACOL_RS11050 50S ribosomal protein L31 type B [1] (data from MRSA252) SACOL_RS11070 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252) SACOL_RS11150 purine-nucleoside phosphorylase [1] (data from MRSA252) SACOL_RS11230 glutamine--fructose-6-phosphate aminotransferase [1] (data from MRSA252) SACOL_RS11370 hypothetical protein [1] (data from MRSA252) SACOL_RS11435 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) SACOL_RS11615 30S ribosomal protein S9 [1] (data from MRSA252) SACOL_RS11620 50S ribosomal protein L13 [1] (data from MRSA252) SACOL_RS11645 50S ribosomal protein L17 [1] (data from MRSA252) SACOL_RS11650 DNA-directed RNA polymerase subunit alpha [1] (data from MRSA252) SACOL_RS11685 50S ribosomal protein L15 [1] (data from MRSA252) SACOL_RS11690 50S ribosomal protein L30 [1] (data from MRSA252) SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252) SACOL_RS11700 50S ribosomal protein L18 [1] (data from MRSA252) SACOL_RS11705 50S ribosomal protein L6 [1] (data from MRSA252) SACOL_RS11710 30S ribosomal protein S8 [1] (data from MRSA252) SACOL_RS11720 50S ribosomal protein L5 [1] (data from MRSA252) SACOL_RS11730 50S ribosomal protein L14 [1] (data from MRSA252) SACOL_RS11735 30S ribosomal protein S17 [1] (data from MRSA252) SACOL_RS11750 30S ribosomal protein S3 [1] (data from MRSA252) SACOL_RS11760 30S ribosomal protein S19 [1] (data from MRSA252) SACOL_RS11765 50S ribosomal protein L2 [1] (data from MRSA252) SACOL_RS11770 50S ribosomal protein L23 [1] (data from MRSA252) SACOL_RS11775 50S ribosomal protein L4 [1] (data from MRSA252) SACOL_RS11785 30S ribosomal protein S10 [1] (data from MRSA252) SACOL_RS12060 octopine dehydrogenase [1] (data from MRSA252) SACOL_RS12675 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [1] (data from MRSA252) SACOL_RS13280 lactate dehydrogenase [1] (data from MRSA252) SACOL_RS13415 hydroxymethylglutaryl-CoA synthase [1] (data from MRSA252) SACOL_RS13460 L-glutamate gamma-semialdehyde dehydrogenase [1] (data from MRSA252) SACOL_RS13695 L-lactate dehydrogenase [1] (data from MRSA252) SACOL_RS13720 class I fructose-bisphosphate aldolase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: CodY see SACOL1505
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)