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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS04925 [old locus tag: SACOL0961 ]
  • pan locus tag?: SAUPAN003060000
  • symbol: SACOL_RS04925
  • pan gene symbol?: gudB
  • synonym:
  • product: NAD-specific glutamate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS04925 [old locus tag: SACOL0961 ]
  • symbol: SACOL_RS04925
  • product: NAD-specific glutamate dehydrogenase
  • replicon: chromosome
  • strand: +
  • coordinates: 962628..963872
  • length: 1245
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (962628..963872) NCBI
  • BioCyc: SACOL_RS04925 BioCyc
  • MicrobesOnline: see SACOL0961

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGACTGAGAACAATAATTTAGTAACTTCTACTCAAGGAATTATTAAAGAAGCATTGCAT
    AAATTGGGATTTGACGAAGGAATGTACGATTTAATTAAAGAACCTTTAAGAATGTTACAA
    GTGCGTATCCCTGTACGAATGGATGATGGCACAGTAAAAACATTCACAGGTTACCGTGCG
    CAACATAATGATGCTGTTGGACCAACAAAAGGGGGCGTGCGTTTCCACCCAGATGTTGAT
    GAAGAAGAAGTAAAAGCATTATCAATGTGGATGACTTTGAAATGTGGCATTGTAAACTTA
    CCATACGGTGGTGGTAAGGGTGGTATCGTTTGTGATCCACGTCAAATGAGCATTCATGAA
    GTTGAACGTTTATCACGCGGATATGTAAGAGCAATTTCACAATTCGTAGGTCCGAACAAA
    GATATTCCAGCACCAGATGTATTTACAAACTCACAAATTATGGCTTGGATGATGGATGAA
    TATAGTGCATTAGATAAATTTAATTCACCAGGTTTCATCACAGGTAAACCAATTGTATTG
    GGTGGTTCTCATGGACGCGACAGATCAACTGCACTAGGTGTAGTTATTGCAATTGAACAA
    GCTGCAAAACGTCGTAATATGCAAATTGAAGGTGCCAAGGTTGTTATTCAAGGTTTCGGT
    AATGCCGGAAGTTTCTTAGCTAAATTCTTATATGATTTAGGTGCAAAAATTGTAGGTATC
    TCTGATGCTTACGGTGCATTACACGATCCAAATGGCTTAGATATAGATTATTTATTAGAC
    CGTCGTGATAGTTTTGGTACGGTAACAAATTTATTTGAAGAAACAATCTCAAATAAAGAA
    TTGTTTGAATTAGATTGTGACATTTTAGTACCAGCGGCTATTTCAAACCAAATTACAGAA
    GACAATGCACATGATATTAAAGCTAGTATCGTTGTTGAAGCTGCTAATGGACCTACAACA
    CCAGAAGCAACACGTATTTTAACTGAACGTGGTATATTATTAGTTCCAGACGTATTAGCA
    AGTGCTGGTGGTGTAACGGTTTCTTACTTCGAATGGGTACAAAATAATCAAGGTTATTAT
    TGGTCTGAAGAAGAAGTTAATGAAAAGCTACGTGAAAAATTAGAAGCGGCATTTGATACG
    ATTTACGAATTGTCTCAAAACCGAAAAATAGATATGAGACTTGCAGCATATATCATAGGT
    ATTAAACGTACAGCAGAAGCAGCTAGATATCGTGGTTGGGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1245

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS04925 [old locus tag: SACOL0961 ]
  • symbol: SACOL_RS04925
  • description: NAD-specific glutamate dehydrogenase
  • length: 414
  • theoretical pI: 5.00453
  • theoretical MW: 45759.7
  • GRAVY: -0.21087

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 1.4.1.2?  ExPASy
    Glutamate dehydrogenase L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH
  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 11.9)
  • TheSEED: see SACOL0961
  • ⊞PFAM:
    NADP_Rossmann (CL0063) ELFV_dehydrog; Glutamate/Leucine/Phenylalanine/Valine dehydrogenase (PF00208; HMM-score: 287.7)
    and 3 more
    AA_dh_N (CL0603) ELFV_dehydrog_N; Glu/Leu/Phe/Val dehydrogenase, dimerisation domain (PF02812; HMM-score: 195.8)
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 16.4)
    ThiF; ThiF family (PF00899; HMM-score: 11.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9672
    • Cytoplasmic Membrane Score: 0.0026
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.0301
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002571
    • TAT(Tat/SPI): 0.000265
    • LIPO(Sec/SPII): 0.000366
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIPVRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPYGGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDEYSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQGFGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETISNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILLVPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDMRLAAYIIGIKRTAEAARYRGWA

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL0961
  • protein localization: see SACOL0961
  • quantitative data / protein copy number per cell: see SACOL0961
  • ⊟interaction partners:
    SACOL_RS06450succinyl-CoA ligase subunit alpha  [1] (data from MRSA252)
    SACOL_RS10090teichoic acid ABC transporter ATP-binding protein  [1] (data from MRSA252)
    SACOL_RS1169530S ribosomal protein S5  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Jump up to: 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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