PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
• pan locus tag^?: SAUPAN003553000
• symbol: SACOL_RS06495
• pan gene symbol^?: codY
• synonym:
• product: GTP-sensing pleiotropic transcriptional regulator CodY

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
• symbol: SACOL_RS06495
• product: GTP-sensing pleiotropic transcriptional regulator CodY
• replicon: chromosome
• strand: +
• coordinates: 1283471..1284244
• length: 774
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1283471..1284244) NCBI
• BioCyc: SACOL_RS06495 BioCyc
• MicrobesOnline: see SACOL1272

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGCTTATTATCTAAAACGAGAGAGTTAAACACGTTACTTCAAAAACACAAAGGTATT
  GCGGTTGATTTTAAAGATGTAGCACAAACGATTAGTAGCGTAACTGTAACAAATGTATTT
  ATTGTATCGCGTCGAGGTAAAATTTTAGGATCGAGTCTAAATGAATTATTAAAAAGTCAA
  AGAATTATTCAAATGTTGGAAGAAAGACATATTCCAAGTGAATATACAGAACGATTAATG
  GAAGTTAAACAAACAGAATCAAATATTGATATCGACAATGTATTAACAGTATTCCCACCT
  GAAAACAGAGAATTATTCATAGATAGTCGTACAACTATCTTCCCAATTTTAGGTGGAGGG
  GAAAGATTAGGTACATTAGTACTTGGTCGAGTACATGATGATTTTAATGAAAATGATTTG
  GTACTAGGTGAATATGCTGCTACAGTTATTGGTATGGAAATCTTACGTGAGAAGCATAGT
  GAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCT
  TATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTA
  TTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCA
  CTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACT
  TTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTAGAAAAAAGTAAATAA
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⊟Protein

• 5EY0
  PDB
• 5EY1
  PDB

Protein Data Bank: 5EY0

Protein Data Bank: 5EY1

⊟General

• locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
• symbol: SACOL_RS06495
• description: GTP-sensing pleiotropic transcriptional regulator CodY
• length: 257
• theoretical pI: 6.07842
• theoretical MW: 28754.9
• GRAVY: -0.178599

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions GTP-sensing transcriptional pleiotropic repressor CodY (TIGR02787; HMM-score: 351.8)
  and 3 more
  Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 20.1)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 15.4)
  Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 14.3)
• TheSEED: see SACOL1272
• PFAM:
  GAF (CL0161) CodY; CodY GAF-like domain (PF06018; HMM-score: 234.2)
  and 21 more
  HTH (CL0123) HTH_CodY; CodY helix-turn-helix domain (PF08222; HMM-score: 104.6)
  Ribosomal_S25; S25 ribosomal protein (PF03297; HMM-score: 23.4)
  HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 20.3)
  GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 18.3)
  HTH_11; HTH domain (PF08279; HMM-score: 17.8)
  HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 17.4)
  TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 17.2)
  Rrf2; Iron-dependent Transcriptional regulator (PF02082; HMM-score: 16.9)
  MarR_2; MarR family (PF12802; HMM-score: 16.9)
  GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 16.3)
  HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 15.4)
  HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 15.3)
  DUF4423; Domain of unknown function (DUF4423) (PF14394; HMM-score: 15.3)
  HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15.1)
  SoFic-like_C; Protein adenylyltransferase SoFic-like, C-terminal domain (PF21248; HMM-score: 13.7)
  GAF (CL0161) GAF; GAF domain (PF01590; HMM-score: 13.3)
  HTH (CL0123) HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 13.3)
  HTH_8; Bacterial regulatory protein, Fis family (PF02954; HMM-score: 12.5)
  no clan defined Coa3_cc; Cytochrome c oxidase assembly factor 3 (PF09813; HMM-score: 12.5)
  HTH (CL0123) HxlR; HxlR-like helix-turn-helix (PF01638; HMM-score: 12.1)
  DUF7343; Domain of unknown function (DUF7343) (PF24034; HMM-score: 10.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:
• genes regulated by CodY, TF important in Amino acid metabolism: see SACOL1272

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9663
  • Cytoplasmic Membrane Score: 0.0296
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.004
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001202
  • TAT(Tat/SPI): 0.000369
  • LIPO(Sec/SPII): 0.000274
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445977482 NCBI
• RefSeq: WP_000055337 NCBI
• UniProt: see SACOL1272

⊟Protein sequence

• MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSRRGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPENRELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHSEVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIVNALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK

⊟Experimental data

• experimentally validated: see SACOL1272
• protein localization: see SACOL1272
• quantitative data / protein copy number per cell: see SACOL1272
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1272

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications