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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

organism: Staphylococcus aureus COL
locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
pan locus tag^?: SAUPAN003553000
symbol: SACOL_RS06495
pan gene symbol^?: codY
synonym:
product: GTP-sensing pleiotropic transcriptional regulator CodY

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
symbol: SACOL_RS06495
product: GTP-sensing pleiotropic transcriptional regulator CodY
replicon: chromosome
strand: +
coordinates: 1283471..1284244
length: 774
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Location: NC_002951 (1283471..1284244) NCBI
BioCyc: SACOL_RS06495 BioCyc
MicrobesOnline: see SACOL1272

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

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721
ATGAGCTTATTATCTAAAACGAGAGAGTTAAACACGTTACTTCAAAAACACAAAGGTATT
GCGGTTGATTTTAAAGATGTAGCACAAACGATTAGTAGCGTAACTGTAACAAATGTATTT
ATTGTATCGCGTCGAGGTAAAATTTTAGGATCGAGTCTAAATGAATTATTAAAAAGTCAA
AGAATTATTCAAATGTTGGAAGAAAGACATATTCCAAGTGAATATACAGAACGATTAATG
GAAGTTAAACAAACAGAATCAAATATTGATATCGACAATGTATTAACAGTATTCCCACCT
GAAAACAGAGAATTATTCATAGATAGTCGTACAACTATCTTCCCAATTTTAGGTGGAGGG
GAAAGATTAGGTACATTAGTACTTGGTCGAGTACATGATGATTTTAATGAAAATGATTTG
GTACTAGGTGAATATGCTGCTACAGTTATTGGTATGGAAATCTTACGTGAGAAGCATAGT
GAAGTAGAAAAAGAAGCGCGCGATAAAGCTGCTATTACAATGGCAATTAATTCATTATCT
TATTCTGAAAAAGAAGCGATTGAACATATCTTTGAAGAACTTGGCGGTACGGAAGGCCTA
TTAATCGCATCAAAAGTTGCAGATAGAGTTGGTATTACTAGATCTGTAATTGTAAATGCA
CTACGTAAATTAGAAAGTGCTGGTGTAATTGAATCACGTTCTTTAGGAATGAAAGGTACT
TTCATTAAAGTTAAAAAAGAAAAATTCTTAGATGAATTAGAAAAAAGTAAATAA
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774

⊟Protein[edit | edit source]

5EY0
PDB
5EY1
PDB

Protein Data Bank: 5EY0

Protein Data Bank: 5EY1

⊟General[edit | edit source]

locus tag: SACOL_RS06495 [old locus tag: SACOL1272 ]
symbol: SACOL_RS06495
description: GTP-sensing pleiotropic transcriptional regulator CodY
length: 257
theoretical pI: 6.07842
theoretical MW: 28754.9
GRAVY: -0.178599

⊟Function[edit | edit source]

TIGRFAM:
Signal transduction Regulatory functions DNA interactions GTP-sensing transcriptional pleiotropic repressor CodY (TIGR02787; HMM-score: 351.8)
and 3 more
Signal transduction Regulatory functions DNA interactions CRISPR locus-related DNA-binding protein (TIGR01884; HMM-score: 20.1)
RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 15.4)
Unknown function General Rrf2 family protein (TIGR00738; HMM-score: 14.3)
TheSEED: see SACOL1272
PFAM:
GAF (CL0161) CodY; CodY GAF-like domain (PF06018; HMM-score: 232.3)
and 15 more
HTH (CL0123) HTH_CodY; CodY helix-turn-helix domain (PF08222; HMM-score: 117.3)
Ribosomal_S25; S25 ribosomal protein (PF03297; HMM-score: 21.6)
MarR_2; MarR family (PF12802; HMM-score: 21.4)
HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 20.5)
Rrf2; Transcriptional regulator (PF02082; HMM-score: 18.4)
GntR; Bacterial regulatory proteins, gntR family (PF00392; HMM-score: 18.3)
HTH_20; Helix-turn-helix domain (PF12840; HMM-score: 18.2)
TrmB; Sugar-specific transcriptional regulator TrmB (PF01978; HMM-score: 17.4)
MarR; MarR family (PF01047; HMM-score: 16.5)
HTH_11; HTH domain (PF08279; HMM-score: 16.4)
HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 15.9)
HTH_41; Helix-turn-helix domain (PF14502; HMM-score: 14.4)
GAF (CL0161) GAF_2; GAF domain (PF13185; HMM-score: 14.3)
no clan defined Coiled-coil_56; Coiled-coil domain-containing protein 56 (PF09813; HMM-score: 13.1)
HTH (CL0123) HxlR; HxlR-like helix-turn-helix (PF01638; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:
genes regulated by CodY, TF important in Amino acid metabolism: see SACOL1272

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001202
- TAT(Tat/SPI): 0.000369
- LIPO(Sec/SPII): 0.000274
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI: 445977482 NCBI
RefSeq: WP_000055337 NCBI
UniProt: see SACOL1272

⊟Protein sequence[edit | edit source]

MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSRRGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPENRELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHSEVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIVNALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK

⊟Experimental data[edit | edit source]

experimentally validated: see SACOL1272
protein localization: see SACOL1272
quantitative data / protein copy number per cell: see SACOL1272
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: see SACOL1272

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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