PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS08865 [old locus tag: SACOL1734 ]
• pan locus tag^?: SAUPAN004309000
• symbol: SACOL_RS08865
• pan gene symbol^?: gapB
• synonym:
• product: aldehyde dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS08865 [old locus tag: SACOL1734 ]
• symbol: SACOL_RS08865
• product: aldehyde dehydrogenase
• replicon: chromosome
• strand: -
• coordinates: 1765067..1766092
• length: 1026
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1765067..1766092) NCBI
• BioCyc: SACOL_RS08865 BioCyc
• MicrobesOnline: see SACOL1734

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  1021
  
  ATGTCAACGAATATTGCAATTAATGGTATGGGTAGAATTGGAAGAATGGTATTACGTATT
  GCATTACAAAATAAAAATTTAAATGTAGTAGCGATAAATGCTAGTTATCCACCCGAAACA
  ATTGCACATTTAATCAATTACGATACGACACATGGAAAATATAATCTAAAAGTTGAACCG
  ATTGAAAATGGATTGCAAGTTGGAGATCATAAAATTAAATTGGTTGCTGATCGCAATCCT
  GAAAACTTGCCATGGAAAGAATTAGATATCGATATTGCTATAGATGCAACTGGTAAATTT
  AATCATGGTGATAAAGCCATCGCACATATTAAAGCAGGTGCCAAAAAAGTTTTGTTAACT
  GGTCCTTCAAAAGGTGGACATGTTCAAATGGTAGTTAAAGGCGTAAATGATAACCAATTA
  GATATAGAAGCATTTGACATTTTTAGTAATGCTTCATGTACTACTAATTGCATTGGTCCA
  GTTGCAAAAGTTTTAAATAATCAGTTTGGAATAGTTAATGGTTTAATGACTACTGTTCAC
  GCTATTACAAATGACCAAAAAAATATTGATAATCCACATAAAGATTTAAGACGTGCACGT
  TCATGTAATGAAAGCATTATTCCTACTTCTACTGGTGCGGCGAAAGCTTTAAAAGAAGTA
  TTACCAGAATTAGAAGGTAAATTACACGGCATGGCATTACGTGTACCAACAAAGAATGTA
  TCGCTCGTTGATTTAGTTGTTGATTTAGAAAAAGAAGTAACTGCAGAAGAAGTAAACCAA
  GCTTTTGAAAATGCAGGTTTAGAAGGTATCATAGAAGTCGAACATCAACCACTAGTGTCT
  GTTGATTTTAATACTAATCCAAATTCAGCTATTATTGATGCAAAATCAACAATGGTCATG
  TCAGGGAATAAAGTAAAAGTTATTGCTTGGTATGATAATGAATGGGGTTATTCAAATAGA
  GTTGTAGATGTTGCTGAGCAAATTGGAGCACTTTTGACATCAAAAGAAACTGTAAGTGCA
  AGTTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS08865 [old locus tag: SACOL1734 ]
• symbol: SACOL_RS08865
• description: aldehyde dehydrogenase
• length: 341
• theoretical pI: 6.35212
• theoretical MW: 36979
• GRAVY: -0.1739

⊟Function

• reaction:
  EC 1.2.1.12?  ExPASy
  Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = 3-phospho-D-glyceroyl phosphate + NADH
• TIGRFAM:
  Metabolism Energy metabolism Glycolysis/gluconeogenesis glyceraldehyde-3-phosphate dehydrogenase, type I (TIGR01534; EC 1.2.1.-; HMM-score: 412.6)
  and 3 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine erythrose-4-phosphate dehydrogenase (TIGR01532; EC 1.2.1.72; HMM-score: 317.7)
  glyceraldehyde-3-phosphate dehydrogenase, type II (TIGR01546; EC 1.2.1.59; HMM-score: 18.2)
  Metabolism Amino acid biosynthesis Aspartate family aspartate-semialdehyde dehydrogenase (TIGR01296; EC 1.2.1.11; HMM-score: 16.3)
• TheSEED: see SACOL1734
• PFAM:
  GADPH_aa-bio_dh (CL0139) Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain (PF02800; HMM-score: 217.2)
  and 4 more
  NADP_Rossmann (CL0063) Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain (PF00044; HMM-score: 121.3)
  DapB_N; Dihydrodipicolinate reductase, N-terminus (PF01113; HMM-score: 16)
  KOW (CL0107) Ribosomal_L14e; Ribosomal protein L14 (PF01929; HMM-score: 13.4)
  NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.056954
  • TAT(Tat/SPI): 0.000955
  • LIPO(Sec/SPII): 0.002552
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446028286 NCBI
• RefSeq: WP_000106141 NCBI
• UniProt: see SACOL1734

⊟Protein sequence

• MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHLINYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNHGDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDNQLDIEAFDIFSNASCTTNCIGPVAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALKEVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQPLVSVDFNTNPNSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTSKETVSAS

⊟Experimental data

• experimentally validated: see SACOL1734
• protein localization: see SACOL1734
• quantitative data / protein copy number per cell:
• interaction partners:

  SACOL_RS03080		elongation factor Tu   (data from MRSA252)
  SACOL_RS04840		NADH dehydrogenase   (data from MRSA252)
  SACOL_RS05640		dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex   (data from MRSA252)
  SACOL_RS06505		30S ribosomal protein S2   (data from MRSA252)
  SACOL_RS08930		pyruvate kinase   (data from MRSA252)
  SACOL_RS08995		universal stress protein UspA   (data from MRSA252)
  SACOL_RS11230		glutamine--fructose-6-phosphate aminotransferase   (data from MRSA252)
  SACOL_RS11685		50S ribosomal protein L15   (data from MRSA252)
  SACOL_RS11750		30S ribosomal protein S3   (data from MRSA252)

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications