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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS03050 [old locus tag: SACOL0588 ]
- pan locus tag?: SAUPAN002314000
- symbol: SACOL_RS03050
- pan gene symbol?: rpoB
- synonym:
- product: DNA-directed RNA polymerase subunit beta
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS03050 [old locus tag: SACOL0588 ]
- symbol: SACOL_RS03050
- product: DNA-directed RNA polymerase subunit beta
- replicon: chromosome
- strand: +
- coordinates: 606988..610539
- length: 3552
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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3541TTGGCAGGTCAAGTTGTCCAATATGGAAGACATCGTAAACGTAGAAACTACGCGAGAATT
TCAGAAGTATTAGAATTACCAAACTTAATAGAAATTCAAACTAAATCTTACGAGTGGTTC
CTAAGAGAAGGTTTAATCGAAATGTTTAGAGACATTTCTCCAATTGAAGATTTTACTGGT
AATTTGTCATTAGAGTTTGTGGATTACCGTTTAGGAGAACCAAAATATGATTTAGAAGAA
TCTAAAAACCGTGACGCTACTTATGCTGCACCTCTTCGTGTAAAAGTGCGTCTAATCATT
AAAGAAACAGGAGAAGTTAAAGAACAAGAAGTCTTTATGGGTGATTTCCCATTAATGACT
GATACAGGTACGTTCGTTATCAATGGTGCAGAACGTGTAATCGTATCTCAATTAGTTCGT
TCACCATCCGTTTATTTCAATGAAAAAATCGACAAAAATGGTCGTGAAAACTATGATGCA
ACAATTATTCCAAACCGTGGTGCATGGTTAGAATATGAAACAGATGCTAAAGATGTTGTA
TACGTACGTATTGATAGAACACGTAAACTACCATTAACAGTATTGTTACGTGCATTAGGT
TTCTCAAGCGACCAAGAAATTGTTGACCTTTTAGGTGACAATGAATATTTACGTAATACT
TTAGAGAAAGACGGCACTGAAAACACTGAACAAGCGTTATTAGAAATCTATGAACGTTTA
CGTCCAGGTGAACCACCAACTGTTGAAAATGCTAAAAGTCTATTGTATTCACGTTTCTTT
GATCCAAAACGCTATGACTTAGCAAGCGTGGGTCGTTATAAAACAAACAAAAAATTACAT
TTAAAACATCGTTTATTTAATCAAAAATTAGCTGAGCCAATTGTAAATACTGAAACTGGT
GAAATTGTAGTTGAAGAAGGTACAGTGCTTGATCGTCGTAAAATCGACGAAATCATGGAT
GTACTTGAATCAAATGCAAACAGCGAAGTGTTTGAATTGCATGGTAGCGTTATAGACGAG
CCAGTAGAAATTCAATCAATTAAAGTATATGTTCCTAACGATGATGAAGGTCGTACGACA
ACTGTAATTGGTAATGCTTTCCCTGACTCAGAAGTTAAATGCATTACACCAGCAGATATC
ATTGCTTCAATGAGTTACTTCTTTAACTTATTAAGCGGTATTGGATATACAGATGATATT
GACCATTTAGGTAACCGTCGTTTACGTTCTGTAGGTGAATTACTACAAAACCAATTCCGT
ATCGGTTTATCAAGAATGGAAAGAGTTGTACGTGAAAGAATGTCAATTCAAGATACTGAG
TCTATCACACCTCAACAATTAATTAATATTCGACCTGTTATTGCATCTATTAAAGAATTC
TTTGGTAGCTCTCAATTATCACAATTCATGGACCAAGCAAACCCATTAGCTGAGTTAACG
CATAAACGTCGTCTATCAGCATTAGGACCTGGTGGTTTAACACGTGAACGTGCTCAAATG
GAAGTACGTGACGTTCACTACTCTCACTATGGCCGTATGTGTCCAATTGAAACACCTGAG
GGACCAAACATTGGATTGATTAACTCATTATCAAGTTATGCACGTGTAAATGAATTCGGC
TTTATTGAAACACCATATCGTAAAGTTGATTTAGATACACATGCTATCACTGATCAAATT
GACTATTTAACAGCTGACGAAGAAGATAGCTATGTTGTAGCACAAGCAAACTCTAAATTA
GATGAAAATGGTCGTTTCATGGATGATGAAGTTGTATGTCGTTTCCGTGGTAACAATACA
GTTATGGCTAAAGAAAAAATGGATTATATGGATGTATCGCCGAAGCAAGTTGTTTCAGCA
GCGACAGCATGTATTCCATTCTTAGAAAATGATGACTCAAACCGTGCATTGATGGGTGCG
AACATGCAACGTCAAGCAGTGCCTTTGATGAATCCAGAAGCACCATTTGTTGGTACAGGT
ATGGAACACGTTGCAGCACGTGATTCTGGTGCGGCTATTACAGCTAAGCACAGAGGTCGT
GTTGAACATGTTGAATCTAATGAAATTCTTGTTCGTCGTCTAGTTGAAGAGAACGGCGTT
GAGCATGAAGGTGAATTAGATCGCTATCCATTAGCTAAATTTAAACGTTCAAACTCAGGT
ACATGTTACAACCAACGTCCAATCGTTGCAGTTGGAGATGTTGTTGAGTATAACGAGATT
TTAGCAGATGGACCATCTATGGAATTAGGAGAAATGGCATTAGGTAGAAACGTAGTAGTT
GGTTTCATGACTTGGGACGGTTACAACTATGAGGATGCCGTTATCATGAGTGAAAGACTT
GTGAAAGATGACGTGTATACTTCTATTCATATTGAAGAGTATGAATCAGAAGTACGTGAT
ACTAAGTTAGGACCTGAAGAAATCACAAGAGATATTCCTAATGTTTCTGAAAGTGCACTT
AAGAACTTAGACGATCGTGGTATCGTTTATATTGGTGCAGAAGTAAAAGATGGAGATATT
TTAGTTGGTAAAGTAACGCCTAAAGGTGTAACTGAGTTAACTGCCGAAGAAAGATTGTTA
CATGCAATCTTTGGTGAAAAAGCACGTGAAGTTAGAGATACTTTATTACGTGTACCTCAC
GGCGCTGGCGGTATCGTTCTTGATGTAAAAGTATTCAATCGTGAAGAAGGCGACGATACA
TTATCACCTGGTGTAAACCAATTAGTACGTGTATATATCGTTCAAAAACGTAAAATTCAT
GTTGGTGATAAGATGTGTGGTCGACATGGTAACAAAGGTGTCATTTCTAAGATTGTTCCT
GAAGAAGATATGCCTTACTTACCAGATGGACGTCCGATCGATATCATGTTAAATCCTCTT
GGTGTACCATCTCGTATGAACATCGGACAAGTATTAGAGCTACACTTAGGTATGGCTGCT
AAAAATCTTGGTATTCACGTTGCATCACCAGTATTTGACGGTGCAAACGATGACGATGTA
TGGTCAACAATTGAAGAAGCTGGTATGGCTCGTGATGGTAAAACTGTACTTTATGATGGA
CGTACAGGTGAACCATTCGATAACCGTATTTCAGTAGGTGTAATGTACATGTTGAAACTT
GCGCACATGGTTGATGATAAATTACATGCGCGTTCAACAGGACCATATTCACTTGTTACA
CAACAACCACTTGGCGGTAAAGCGCAATTCGGTGGACAACGTTTTGGTGAGATGGAGGTA
TGGGCACTTGAAGCATATGGTGCTGCATACACATTACAAGAAATCTTAACTTACAAATCC
GATGATACAGTAGGACGTGTGAAAACATACGAGGCTATTGTTAAAGGTGAAAACATCTCT
AGACCAAGTGTTCCAGAATCATTCCGAGTATTGATGAAAGAATTACAAAGTTTAGGTTTA
GATGTAAAAGTTATGGATGAGCAAGATAATGAAATCGAAATGACAGACGTTGATGACGAT
GATGTTGTAGAACGCAAAGTAGATTTACAACAAAATGATGCTCCTGAAACACAAAAAGAA
GTTACTGATTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS03050 [old locus tag: SACOL0588 ]
- symbol: SACOL_RS03050
- description: DNA-directed RNA polymerase subunit beta
- length: 1183
- theoretical pI: 4.6446
- theoretical MW: 133273
- GRAVY: -0.428402
⊟Function[edit | edit source]
- reaction: EC 2.7.7.6? ExPASyDNA-directed RNA polymerase Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
- TIGRFAM: Transcription DNA-dependent RNA polymerase DNA-directed RNA polymerase, beta subunit (TIGR02013; EC 2.7.7.6; HMM-score: 1804.1)and 1 moreDNA-directed RNA polymerase subunit B (TIGR03670; EC 2.7.7.6; HMM-score: 295.2)
- TheSEED: see SACOL0588
- PFAM: LEF-8-like (CL0410) RNA_pol_Rpb2_6; RNA polymerase Rpb2, domain 6 (PF00562; HMM-score: 547.8)and 5 moreno clan defined RNA_pol_Rpb2_2; RNA polymerase Rpb2, domain 2 (PF04561; HMM-score: 124.8)RNA_pol_Rpb2_3; RNA polymerase Rpb2, domain 3 (PF04565; HMM-score: 104.2)RNA_pol_Rpb2_1; RNA polymerase beta subunit (PF04563; HMM-score: 103.4)RNA_pol_Rpb2_45; RNA polymerase beta subunit external 1 domain (PF10385; HMM-score: 91.7)RNA_pol_Rpb2_7; RNA polymerase Rpb2, domain 7 (PF04560; HMM-score: 91.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001131
- TAT(Tat/SPI): 0.000126
- LIPO(Sec/SPII): 0.000187
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREGLIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKETGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDATIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLRNTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTNKKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELHGSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLSGIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINIRPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYSHYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTADEEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATACIPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVEHVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEILADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEVRDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAEERLLHAIFGEKAREVRDTLLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIVQKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVLELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRISVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMDEQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL0588
- protein localization: see SACOL0588
- quantitative data / protein copy number per cell: see SACOL0588
- interaction partners:
SACOL_RS01040 formate acetyltransferase [1] (data from MRSA252) SACOL_RS02950 excinuclease ABC subunit B [1] (data from MRSA252) SACOL_RS02955 protein arginine kinase [1] (data from MRSA252) SACOL_RS03080 elongation factor Tu [1] (data from MRSA252) SACOL_RS08995 universal stress protein UspA [1] (data from MRSA252) SACOL_RS09005 class I SAM-dependent methyltransferase [1] (data from MRSA252) SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)