From AureoWiki
Jump to navigation Jump to search

NCBI: 02-MAR-2017

Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS02955 [old locus tag: SACOL0569 ]
  • pan locus tag?: SAUPAN002289000
  • symbol: SACOL_RS02955
  • pan gene symbol?: mcsB
  • synonym:
  • product: protein arginine kinase

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS02955 [old locus tag: SACOL0569 ]
  • symbol: SACOL_RS02955
  • product: protein arginine kinase
  • replicon: chromosome
  • strand: +
  • coordinates: 589032..590039
  • length: 1008
  • essential: unknown other strains

Accession numbers[edit | edit source]

  • Location: NC_002951 (589032..590039) NCBI
  • BioCyc: SACOL_RS02955 BioCyc
  • MicrobesOnline: see SACOL0569

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACGCATAATATTCATGATAATATCAGCCAATGGATGAAAAGTAATGAAGAAACACCA
    ATTGTTATGTCTTCTAGAATTCGGTTAGCGCGTAATTTAGAAAATCATGTGCATCCACTA
    ATGTATGCTACTGAAAATGATGGATTTAGAGTTATAAATGAGGTACAAGATGCCTTGCCA
    AACTTTGAATTAATGCGTCTTGATCAAATGGATCAACAAAGTAAAATGAAAATGGTTGCA
    AAGCATTTGATTAGTCCTGAACTAATAAAACAACCAGCAGCCGCAGTATTAGTGAATGAC
    GATGAATCTTTAAGTGTCATGATAAATGAAGAGGACCATATTCGTATTCAAGCTATGGGA
    ACTGACACGACATTACAGGCTTTATATAATCAAGCTTCATCAATTGATGATGAATTAGAT
    CGAAGCCTTGATATAAGTTATGATGAACAACTTGGTTATTTAACTACATGTCCTACCAAT
    ATAGGTACTGGTATGAGAGCAAGCGTGATGCTACATTTGCCAGGTCTATCTATTATGAAA
    AGAATGACACGGATTGCTCAAACCATTAATCGTTTTGGATATACAATCAGAGGTATTTAC
    GGTGAAGGTTCGCAAGTTTATGGACATACTTATCAAGTATCCAACCAACTTACACTTGGT
    AAATCTGAGTTAGAAATCATAGAAACATTAACAGAAGTTGTTAATCAAATCATTCATGAA
    GAAAAACAAATACGACAAAAGTTAGACACTTATAATCAATTAGAAACACAAGACCGTGTT
    TTTCGCTCGCTAGGTATTTTACAAAACTGTAGAATGATAACTATGGAAGAGGCTTCTTAT
    AGATTAAGCGAAGTTAAACTTGGTATAGATTTAAATTACATTGAATTACAAAACTTTAAA
    TTTAATGAATTGATGGTAGCTATACAGTCACCATTTTTATTAGATGAAGAAGATGACAAA
    TCTGTAAAAGAAAAACGAGCAGATATACTAAGAGAACATATAAAGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SACOL_RS02955 [old locus tag: SACOL0569 ]
  • symbol: SACOL_RS02955
  • description: protein arginine kinase
  • length: 335
  • theoretical pI: 4.87485
  • theoretical MW: 38609.8
  • GRAVY: -0.417612

Function[edit | edit source]

  • reaction:
    EC 2.7.14.1?  ExPASy
    Protein arginine kinase ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine
  • TIGRFAM:
  • TheSEED: see SACOL0569
  • PFAM:
    GCS (CL0286) ATP-gua_Ptrans; ATP:guanido phosphotransferase, C-terminal catalytic domain (PF00217; HMM-score: 224.2)
    and 1 more
    no clan defined Pas_Saposin; Pas factor saposin fold (PF09016; HMM-score: 11.8)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002519
    • TAT(Tat/SPI): 0.00016
    • LIPO(Sec/SPII): 0.000297
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYATENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDESLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTNIGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLTLGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITMEEASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHIK

Experimental data[edit | edit source]

  • experimentally validated: see SACOL0569
  • protein localization: see SACOL0569
  • quantitative data / protein copy number per cell: see SACOL0569
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • data available for N315

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]