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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0482 [new locus tag: SA_RS02835 ]
- pan locus tag?: SAUPAN002289000
- symbol: SA0482
- pan gene symbol?: mcsB
- synonym:
- product: ATP:guanido phosphotransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0482 [new locus tag: SA_RS02835 ]
- symbol: SA0482
- product: ATP:guanido phosphotransferase
- replicon: chromosome
- strand: +
- coordinates: 561665..562672
- length: 1008
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1123286 NCBI
- RefSeq: NP_373734 NCBI
- BioCyc: see SA_RS02835
- MicrobesOnline: 102760 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGACGCATAATATTCATGATAATATCAGCCAATGGATGAAAAGTAATGAAGAAACACCA
ATTGTTATGTCTTCTAGAATTCGGTTAGCGCGTAATTTAGAAAATCATGTGCATCCACTA
ATGTATGCTACTGAAAATGATGGATTTAGAGTTATAAATGAGGTACAAGATGCCTTGCCA
AATTTTGAATTAATGCGTCTTGATCAAATGGATCAACAAAGTAAAATGAAAATGGTTGCA
AAGCATTTGATTAGTCCTGAACTAATAAAACAACCAGCAGCCGCAGTATTAGTGAATGAT
GATGAATCTTTAAGTGTCATGATAAATGAAGAGGACCATATTCGTATTCAAGCTATGGGA
ACTGACACGACATTACAGGCTTTATATAATCAAGCTTCATCAATTGATGATGAATTAGAT
CGAAGCCTTGATATAAGTTATGATGAACAACTTGGTTATTTAACTACATGTCCTACCAAT
ATAGGTACTGGTATGAGAGCAAGCGTGATGCTACATTTACCAGGTCTATCTATTATGAAA
AGAATGACACGGATTGCTCAAACCATTAATCGTTTTGGATATACAATCAGAGGTATTTAC
GGTGAAGGTTCGCAAGTTTATGGACATACTTATCAAGTATCCAACCAACTTACACTTGGT
AAATCTGAGTTAGAAATCATAGAAACATTAACAGAAGTTGTTAATCAAATCATTCATGAT
GAAAAACAAATACGACAAAAGTTAGACACTTATAATCAATTAGAAACACAAGACCGTGTT
TTTCGCTCGCTAGGTATTTTACAAAACTGTAGAATGATAACTATGGAAGAGGCTTCTTAT
AGATTAAGCGAAGTTAAACTTGGTATAGATTTAAATTACATTGAATTACAAAACTTTAAA
TTTAATGAATTGATGGTAGCTATACAGTCACCATTTTTATTAGATGAAGAAGATGACAAA
TCTGTAAAAGAAAAACGAGCAGATATACTAAGAGAACATATAAAGTAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0482 [new locus tag: SA_RS02835 ]
- symbol: SA0482
- description: ATP:guanido phosphotransferase
- length: 335
- theoretical pI: 4.87113
- theoretical MW: 38595.7
- GRAVY: -0.417612
⊟Function[edit | edit source]
- reaction: EC 2.7.14.1? ExPASyProtein arginine kinase ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine
- TIGRFAM:
- TheSEED :
- Protein-arginine kinase McsB (EC 2.7.14.1)
- PFAM: GCS (CL0286) ATP-gua_Ptrans; ATP:guanido phosphotransferase, C-terminal catalytic domain (PF00217; HMM-score: 223.5)and 1 moreno clan defined Pas_Saposin; Pas factor saposin fold (PF09016; HMM-score: 11.9)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002519
- TAT(Tat/SPI): 0.00016
- LIPO(Sec/SPII): 0.000297
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYATENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDESLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTNIGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLTLGKSELEIIETLTEVVNQIIHDEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITMEEASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHIK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: ctsR > SA0481 > SA0482 > clpC
⊟Regulation[edit | edit source]
- regulator: CtsR (repression) regulon
CtsR (TF) important in Heat shock response; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Darren J Wozniak, Kiran B Tiwari, Rami Soufan, Radheshyam K Jayaswal
The mcsB gene of the clpC operon is required for stress tolerance and virulence in Staphylococcus aureus.
Microbiology (Reading): 2012, 158(Pt 10);2568-2576
[PubMed:22902728] [WorldCat.org] [DOI] (I p)