PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
• pan locus tag^?: SAUPAN002289000
• symbol: NWMN_0486
• pan gene symbol^?: mcsB
• synonym:
• product: ATP:guanido phosphotransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
• symbol: NWMN_0486
• product: ATP:guanido phosphotransferase
• replicon: chromosome
• strand: +
• coordinates: 558119..559129
• length: 1011
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330258 NCBI
• RefSeq: YP_001331520 NCBI
• BioCyc:
• MicrobesOnline: 3706033 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGATGACGCATAATATTCATGATAATATCAGCCAATGGATGAAAAGTAATGAAGAAACA
  CCAATTGTTATGTCTTCTAGAATTCGGTTAGCGCGTAATTTAGAAAATCATGTGCATCCA
  CTAATGTATGCTACTGAAAATGATGGATTTAGAGTTATAAATGAGGTACAAGATGCCTTG
  CCAAACTTTGAATTAATGCGTCTTGATCAAATGGATCAACAAAGTAAAATGAAAATGGTT
  GCAAAGCATTTGATTAGTCCTGAACTAATAAAACAACCAGCAGCCGCAGTATTAGTGAAT
  GACGATGAATCTTTAAGTGTCATGATAAATGAAGAGGACCATATTCGTATTCAAGCTATG
  GGAACTGACACGACATTACAGGCTTTATATAATCAAGCTTCATCAATTGATGATGAATTA
  GATCGAAGCCTTGATATAAGTTATGATGAACAACTTGGTTATTTAACTACATGTCCTACC
  AATATAGGTACTGGTATGAGAGCAAGCGTGATGCTACATTTGCCAGGTCTATCTATTATG
  AAAAGAATGACACGGATTGCTCAAACCATTAATCGTTTTGGATATACAATCAGAGGTATT
  TACGGTGAAGGTTCGCAAGTTTATGGACATACTTATCAAGTATCCAACCAACTTACACTT
  GGTAAATCTGAGTTAGAAATCATAGAAACATTAACAGAAGTTGTTAATCAAATCATTCAT
  GAAGAAAAACAAATACGACAAAAGTTAGACACTTATAATCAATTAGAAACACAAGACCGT
  GTTTTTCGCTCGCTAGGTATTTTACAAAACTGTAGAATGATAACTATGGAAGAGGCTTCT
  TATAGATTAAGCGAAGTTAAACTTGGTATAGATTTAAATTACATTGAATTACAAAACTTT
  AAATTTAATGAATTGATGGTAGCTATACAGTCACCATTTTTATTAGATGAAGAAGATGAC
  AAATCTGTAAAAGAAAAACGAGCAGATATACTAAGAGAACATATAAAGTAG
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⊟Protein

⊟General

• locus tag: NWMN_0486 [new locus tag: NWMN_RS02840 ]
• symbol: NWMN_0486
• description: ATP:guanido phosphotransferase
• length: 336
• theoretical pI: 4.87485
• theoretical MW: 38741
• GRAVY: -0.410714

⊟Function

• reaction:
  EC 2.7.14.1?  ExPASy
  Protein arginine kinase ATP + a [protein]-L-arginine = ADP + a [protein]-N(omega)-phospho-L-arginine
• TIGRFAM:
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  GCS (CL0286) ATP-gua_Ptrans; ATP:guanido phosphotransferase, C-terminal catalytic domain (PF00217; HMM-score: 224.2)
  and 1 more
  no clan defined Pas_Saposin; Pas factor saposin fold (PF09016; HMM-score: 11.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002442
  • TAT(Tat/SPI): 0.000181
  • LIPO(Sec/SPII): 0.000286
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220698 NCBI
• RefSeq: YP_001331520 NCBI
• UniProt: A0A0H3KEK5 UniProt

⊟Protein sequence

• MMTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYATENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDESLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDRSLDISYDEQLGYLTTCPTNIGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLTLGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITMEEASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHIK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ctsR > NWMN_0485 > NWMN_0486 > clpC

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Darren J Wozniak, Kiran B Tiwari, Rami Soufan, Radheshyam K Jayaswal
The mcsB gene of the clpC operon is required for stress tolerance and virulence in Staphylococcus aureus.
Microbiology (Reading): 2012, 158(Pt 10);2568-2576
[PubMed:22902728] [WorldCat.org] [DOI] (I p)