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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS04330 [old locus tag: SACOL0842 ]
- pan locus tag?: SAUPAN002710000
- symbol: SACOL_RS04330
- pan gene symbol?: eno
- synonym:
- product: enolase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS04330 [old locus tag: SACOL0842 ]
- symbol: SACOL_RS04330
- product: enolase
- replicon: chromosome
- strand: +
- coordinates: 869714..871018
- length: 1305
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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1261ATGCCAATTATTACAGATGTTTACGCTCGCGAAGTCTTAGACTCTCGTGGTAACCCAACT
GTTGAAGTAGAAGTATTAACTGAAAGTGGCGCATTTGGTCGTGCATTAGTACCATCAGGT
GCTTCAACTGGTGAACACGAAGCTGTTGAATTACGTGATGGAGACAAATCACGTTATTTA
GGTAAAGGTGTTACTAAAGCAGTTGAAAACGTTAATGAAATCATCGCACCAGAAATTATT
GAAGGTGAATTTTCAGTATTAGATCAAGTATCTATTGATAAAATGATGATCGCATTAGAC
GGTACTCCAAACAAAGGTAAATTAGGTGCAAATGCTATTTTAGGTGTATCTATCGCAGTA
GCACGTGCAGCAGCTGACTTATTAGGTCAACCACTTTACAAATATTTAGGTGGATTTAAT
GGTAAGCAGTTACCAGTACCAATGATGAACATCGTTAATGGTGGTTCTCACTCAGATGCT
CCAATTGCATTCCAAGAATTCATGATTTTACCTGTAGGTGCTACAACGTTCAAAGAATCA
TTACGTTGGGGTACTGAAATTTTCCACAACTTAAAATCAATTTTAAGCAAACGTGGTTTA
GAAACTGCAGTAGGTGACGAAGGTGGTTTCGCTCCTAAATTTGAAGGTACTGAAGATGCT
GTTGAAACAATTATCCAAGCAATCGAAGCAGCTGGTTACAAACCAGGTGAAGAAGTATTC
TTAGGATTTGACTGTGCATCATCAGAATTCTATGAAAATGGTGTATATGACTACAGTAAG
TTCGAAGGCGAACACGGTGCAAAACGTACAGCTGCAGAACAAGTTGACTACTTAGAACAA
TTAGTAGACAAATATCCTATCATTACAATTGAAGACGGTATGGACGAAAACGACTGGGAT
GGTTGGAAACAACTTACAGAACGTATCGGTGACCGTGTACAATTAGTAGGTGACGATTTA
TTCGTAACAAACACTGAAATTTTAGCAAAAGGTATTGAAAACGGAATTGGTAACTCAATC
TTAATTAAAGTTAACCAAATCGGTACATTAACTGAAACATTTGATGCAATCGAAATGGCT
CAAAAAGCTGGTTACACAGCAGTAGTTTCTCACCGTTCAGGTGAAACAGAAGATACAACA
ATTGCTGATATTGCTGTTGCTACAAACGCTGGTCAAATTAAAACTGGTTCATTATCACGT
ACTGACCGTATTGCTAAATACAATCAATTATTACGTATCGAAGATGAATTATTTGAAACT
GCTAAATATGACGGTATCAAATCATTCTATAACTTAGATAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS04330 [old locus tag: SACOL0842 ]
- symbol: SACOL_RS04330
- description: enolase
- length: 434
- theoretical pI: 4.27961
- theoretical MW: 47116.6
- GRAVY: -0.200461
⊟Function[edit | edit source]
- reaction: EC 4.2.1.11? ExPASyPhosphopyruvate hydratase 2-phospho-D-glycerate = phosphoenolpyruvate + H2O
- TIGRFAM: Energy metabolism Glycolysis/gluconeogenesis phosphopyruvate hydratase (TIGR01060; EC 4.2.1.11; HMM-score: 690.8)and 3 moreBiosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 17.8)Energy metabolism Fermentation methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)Energy metabolism Amino acids and amines methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
- TheSEED: see SACOL0842
- PFAM: Enolase_TIM (CL0256) Enolase_C; Enolase, C-terminal TIM barrel domain (PF00113; HMM-score: 434.7)and 3 moreEnolase_N (CL0227) Enolase_N; Enolase, N-terminal domain (PF03952; HMM-score: 204.8)Enolase_TIM (CL0256) MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 26.1)MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 25.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: Mg2+
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.005297
- TAT(Tat/SPI): 0.001834
- LIPO(Sec/SPII): 0.000693
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL0842
- protein localization: see SACOL0842
- quantitative data / protein copy number per cell: see SACOL0842
- interaction partners:
SACOL_RS02145 acetyl-CoA acyltransferase [1] (data from MRSA252) SACOL_RS03755 LysR family transcriptional regulator [1] (data from MRSA252) SACOL_RS05165 NAD(+) kinase [1] (data from MRSA252) SACOL_RS11615 30S ribosomal protein S9 [1] (data from MRSA252) SACOL_RS11645 50S ribosomal protein L17 [1] (data from MRSA252) SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252) SACOL_RS11745 50S ribosomal protein L16 [1] (data from MRSA252) SACOL_RS11770 50S ribosomal protein L23 [1] (data from MRSA252) SACOL_RS11775 50S ribosomal protein L4 [1] (data from MRSA252) SACOL_RS12085 hypothetical protein [1] (data from MRSA252) SACOL_RS12225 LysR family transcriptional regulator [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: GapR see SACOL0842
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)