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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0760 [new locus tag: SAUSA300_RS04100 ]
  • pan locus tag?: SAUPAN002710000
  • symbol: eno
  • pan gene symbol?: eno
  • synonym:
  • product: phosphopyruvate hydratase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0760 [new locus tag: SAUSA300_RS04100 ]
  • symbol: eno
  • product: phosphopyruvate hydratase
  • replicon: chromosome
  • strand: +
  • coordinates: 848219..849523
  • length: 1305
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCAATTATTACAGATGTTTACGCTCGCGAAGTCTTAGACTCTCGTGGTAACCCAACT
    GTTGAAGTAGAAGTATTAACTGAAAGTGGCGCATTTGGTCGTGCATTAGTACCATCAGGT
    GCTTCAACTGGTGAACACGAAGCTGTTGAATTACGTGATGGAGACAAATCACGTTATTTA
    GGTAAAGGTGTTACTAAAGCAGTTGAAAACGTTAATGAAATCATCGCACCAGAAATTATT
    GAAGGTGAATTTTCAGTATTAGATCAAGTATCTATTGATAAAATGATGATCGCATTAGAC
    GGTACTCCAAACAAAGGTAAATTAGGTGCAAATGCTATTTTAGGTGTATCTATCGCAGTA
    GCACGTGCAGCAGCTGACTTATTAGGTCAACCACTTTACAAATATTTAGGTGGATTTAAT
    GGTAAGCAGTTACCAGTACCAATGATGAACATCGTTAATGGTGGTTCTCACTCAGATGCT
    CCAATTGCATTCCAAGAATTCATGATTTTACCTGTAGGTGCTACAACGTTCAAAGAATCA
    TTACGTTGGGGTACTGAAATTTTCCACAACTTAAAATCAATTTTAAGCAAACGTGGTTTA
    GAAACTGCAGTAGGTGACGAAGGTGGTTTCGCTCCTAAATTTGAAGGTACTGAAGATGCT
    GTTGAAACAATTATCCAAGCAATCGAAGCAGCTGGTTACAAACCAGGTGAAGAAGTATTC
    TTAGGATTTGACTGTGCATCATCAGAATTCTATGAAAATGGTGTATATGACTACAGTAAG
    TTCGAAGGCGAACACGGTGCAAAACGTACAGCTGCAGAACAAGTTGACTACTTAGAACAA
    TTAGTAGACAAATATCCTATCATTACAATTGAAGACGGTATGGACGAAAACGACTGGGAT
    GGTTGGAAACAACTTACAGAACGTATCGGTGACCGTGTACAATTAGTAGGTGACGATTTA
    TTCGTAACAAACACTGAAATTTTAGCAAAAGGTATTGAAAACGGAATTGGTAACTCAATC
    TTAATTAAAGTTAACCAAATCGGTACATTAACTGAAACATTTGATGCAATCGAAATGGCT
    CAAAAAGCTGGTTACACAGCAGTAGTTTCTCACCGTTCAGGTGAAACAGAAGATACAACA
    ATTGCTGATATTGCTGTTGCTACAAACGCTGGTCAAATTAAAACTGGTTCATTATCACGT
    ACTGACCGTATTGCTAAATACAATCAATTATTACGTATCGAAGATGAATTATTTGAAACT
    GCTAAATATGACGGTATCAAATCATTCTATAACTTAGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1305

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0760 [new locus tag: SAUSA300_RS04100 ]
  • symbol: Eno
  • description: phosphopyruvate hydratase
  • length: 434
  • theoretical pI: 4.27961
  • theoretical MW: 47116.6
  • GRAVY: -0.200461

⊟Function[edit | edit source]

  • reaction:
    EC 4.2.1.11?  ExPASy
    Phosphopyruvate hydratase 2-phospho-D-glycerate = phosphoenolpyruvate + H2O
  • TIGRFAM:
    Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphopyruvate hydratase (TIGR01060; EC 4.2.1.11; HMM-score: 690.8)
    and 3 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 17.8)
    Metabolism Energy metabolism Fermentation methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
    Metabolism Energy metabolism Amino acids and amines methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
  • TheSEED  :
    • Enolase (EC 4.2.1.11)
    Carbohydrates Central carbohydrate metabolism Dehydrogenase complexes  Enolase (EC 4.2.1.11)
    and 2 more
    Carbohydrates Central carbohydrate metabolism Glycolysis and Gluconeogenesis  Enolase (EC 4.2.1.11)
    Carbohydrates One-carbon Metabolism Serine-glyoxylate cycle  Enolase (EC 4.2.1.11)
  • PFAM:
    Enolase_TIM (CL0256) Enolase_C; Enolase, C-terminal TIM barrel domain (PF00113; HMM-score: 434.7)
    and 3 more
    Enolase_N (CL0227) Enolase_N; Enolase, N-terminal domain (PF03952; HMM-score: 204.8)
    Enolase_TIM (CL0256) MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 26.1)
    MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 25.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005297
    • TAT(Tat/SPI): 0.001834
    • LIPO(Sec/SPII): 0.000693
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    SAUSA300_0908(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SAUSA300_2203(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SAUSA300_2197(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    SAUSA300_2177(rplQ)50S ribosomal protein L17  [1] (data from MRSA252)
    SAUSA300_2202(rplW)50S ribosomal protein L23  [1] (data from MRSA252)
    SAUSA300_2187(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SAUSA300_2171(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)
    SAUSA300_0355acetyl-CoA acetyltransferase  [1] (data from MRSA252)
    SAUSA300_0658LysR family transcriptional regulator  [1] (data from MRSA252)
    SAUSA300_2255hypothetical protein  [1] (data from MRSA252)
    SAUSA300_2279LysR family regulatory protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: GapR* (repression) regulon
    GapR*(TF)important in Glycolysis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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