PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_000750
• pan locus tag^?: SAUPAN002710000
• symbol: eno
• pan gene symbol^?: eno
• synonym:
• product: surface-displayed alpha-enolase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_000750
• symbol: eno
• product: surface-displayed alpha-enolase
• replicon: chromosome
• strand: +
• coordinates: 789395..790699
• length: 1305
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  
  ATGCCAATTATTACAGATGTTTACGCTCGCGAAGTCTTAGACTCTCGTGGTAACCCAACT
  GTTGAAGTAGAAGTATTAACTGAAAGTGGCGCATTTGGTCGTGCATTAGTACCATCAGGT
  GCTTCAACTGGTGAACACGAAGCTGTTGAATTACGTGATGGAGACAAATCACGTTATTTA
  GGTAAAGGTGTTACTAAAGCAGTTGAAAACGTTAATGAAATCATCGCACCAGAAATTATT
  GAAGGTGAATTTTCAGTATTAGATCAAGTATCTATTGATAAAATGATGATCGCATTAGAC
  GGTACTCCAAACAAAGGTAAATTAGGTGCAAATGCTATTTTAGGTGTATCTATCGCAGTA
  GCACGTGCAGCAGCTGACTTATTAGGTCAACCACTTTACAAATATTTAGGTGGATTTAAT
  GGTAAGCAGTTACCAGTACCAATGATGAACATCGTTAATGGTGGTTCTCACTCAGATGCT
  CCAATTGCATTCCAAGAATTCATGATTTTACCTGTAGGTGCTACAACGTTCAAAGAATCA
  TTACGTTGGGGTACTGAAATTTTCCACAACTTAAAATCAATTTTAAGCAAACGTGGTTTA
  GAAACTGCAGTAGGTGACGAAGGTGGTTTCGCTCCTAAATTTGAAGGTACTGAAGATGCT
  GTTGAAACAATTATCCAAGCAATCGAAGCAGCTGGTTACAAACCAGGTGAAGAAGTATTC
  TTAGGATTTGACTGTGCATCATCAGAATTCTATGAAAATGGTGTATATGACTACAGTAAG
  TTCGAAGGCGAACACGGTGCAAAACGTACAGCTGCAGAACAAGTTGACTACTTAGAACAA
  TTAGTAGACAAATATCCTATCATTACAATTGAAGACGGTATGGACGAAAACGACTGGGAT
  GGTTGGAAACAACTTACAGAACGTATCGGTGACCGTGTACAATTAGTAGGTGACGATTTA
  TTCGTAACAAACACTGAAATTTTAGCAAAAGGTATTGAAAACGGAATTGGTAACTCAATC
  TTAATTAAAGTTAACCAAATCGGTACATTAACTGAAACATTTGATGCAATCGAAATGGCT
  CAAAAAGCTGGTTACACAGCAGTAGTTTCTCACCGTTCAGGTGAAACAGAAGATACAACA
  ATTGCTGATATTGCTGTTGCTACAAACGCTGGTCAAATTAAAACTGGTTCATTATCACGT
  ACTGACCGTATTGCTAAATACAATCAATTATTACGTATCGAAGATGAATTATTTGAAACT
  GCTAAATATGACGGTATCAAATCATTCTATAACTTAGATAAATAA
  60
  120
  180
  240
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  360
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  480
  540
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  660
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  840
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  960
  1020
  1080
  1140
  1200
  1260
  1305
  

⊟Protein

⊟General

• locus tag: JSNZ_000750
• symbol: Eno
• description: surface-displayed alpha-enolase
• length: 434
• theoretical pI: 4.27961
• theoretical MW: 47116.6
• GRAVY: -0.200461

⊟Function

• reaction:
  EC 4.2.1.11?  ExPASy
  Phosphopyruvate hydratase 2-phospho-D-glycerate = phosphoenolpyruvate + H₂O
• TIGRFAM:
  Metabolism Energy metabolism Glycolysis/gluconeogenesis phosphopyruvate hydratase (TIGR01060; EC 4.2.1.11; HMM-score: 690.8)
  and 3 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone o-succinylbenzoate synthase (TIGR01928; EC 4.2.1.113; HMM-score: 17.8)
  Metabolism Energy metabolism Fermentation methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
  Metabolism Energy metabolism Amino acids and amines methylaspartate ammonia-lyase (TIGR01502; EC 4.3.1.2; HMM-score: 16.8)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  TIM_barrel (CL0036) Enolase_C; Enolase, C-terminal TIM barrel domain (PF00113; HMM-score: 439.1)
  and 4 more
  Enolase_N (CL0227) Enolase_N; Enolase, N-terminal domain (PF03952; HMM-score: 206.3)
  TIM_barrel (CL0036) MAAL_C; Methylaspartate ammonia-lyase C-terminus (PF07476; HMM-score: 26)
  MR_MLE_C; Enolase C-terminal domain-like (PF13378; HMM-score: 25)
  TetR_C (CL0174) TetR_C_17; Tetracyclin repressor-like, C-terminal domain (PF17922; HMM-score: 13.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9818
  • Cytoplasmic Membrane Score: 0.0007
  • Cell wall & surface Score: 0.0005
  • Extracellular Score: 0.017
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005297
  • TAT(Tat/SPI): 0.001834
  • LIPO(Sec/SPII): 0.000693
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications